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Akira Matsuno,
Akiko Mizutani,
Hiroko Okinaga,
Koji Takano,
So Yamada,
Shoko M Yamada,
Hiroshi Nakaguchi,
Katsumi Hoya,
Mineko Murakami, Masato Takeuchi,
Mutsumi Sugaya,
Johbu Itoh,
Susumu Takekoshi,
R Yoshiyuki Osamura
[show abstract]
[hide abstract]
ABSTRACT: In the original manuscript, the word "fluorescein" was erroneously used indistinctly for "fluorescence" and "fluorescent". Furthermore, "cyan fluorescent protein" was misspelled. These errors have been amended in an amended version of the manuscript, which is available from the Molecules website. The authors and publisher apologize for the inconvenience.
Molecules 01/2012; 17(10):11667-8. · 2.39 Impact Factor
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Akira Matsuno,
Akiko Mizutani,
Hiroko Okinaga,
Koji Takano,
So Yamada,
Shoko M Yamada,
Hiroshi Nakaguchi,
Katsumi Hoya,
Mineko Murakami, Masato Takeuchi,
Mutsumi Sugaya,
Johbu Itoh,
Susumu Takekoshi,
R Yoshiyuki Osamura
[show abstract]
[hide abstract]
ABSTRACT: Combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy (EM-ISH & IHC) has sufficient ultrastructural resolution to provide two-dimensional images of subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (Quantum dots; Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of the subcellular localization of pituitary hormone and its mRNA. Both EM-ISH & IHC and ISH & IHC using Qdots and CLSM are useful for understanding the relationship between protein and mRNA simultaneously in two or three dimensions. CLSM observation of rab3B and SNARE proteins such as SNAP-25 and syntaxin revealed that both rab3B and SNARE system proteins play an important role and work together as the exocytotic machinery in anterior pituitary cells. Another important issue is the intracellular transport and secretion of pituitary hormone. An experimental pituitary cell line, the GH3 cell, in which growth hormone (GH) is linked to enhanced yellow fluorescein protein (EYFP), has been developed. This stable GH3 cell secretes GH linked to EYFP upon being stimulated by Ca(2+) influx or Ca(2+) release from storage. This GH3 cell is useful for real-time visualization of the intracellular transport and secretion of GH. These three methods enable us to visualize consecutively the processes of transcription, translation, transport, and secretion of pituitary hormone.
Medical Molecular Morphology 06/2011; 44(2):63-70. · 1.39 Impact Factor
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Akira Matsuno,
Akiko Mizutani,
Hiroko Okinaga,
Koji Takano,
So Yamada,
Shoko M Yamada,
Hiroshi Nakaguchi,
Katsumi Hoya,
Mineko Murakami, Masato Takeuchi,
Mutsumi Sugaya,
Johbu Itoh,
Susumu Takekoshi,
R Yoshiyuki Osamura
[show abstract]
[hide abstract]
ABSTRACT: In situ hybridization (ISH) at the electron microscopic (EM) level is essential for elucidating the intracellular distribution and role of mRNA in protein synthesis. EM-ISH is considered to be an important tool for clarifying the intracellular localization of mRNA and the exact site of pituitary hormone synthesis on the rough endoplasmic reticulum. A combined ISH and immunohistochemistry (IHC) under EM (EM-ISH&IHC) approach has sufficient ultrastructural resolution, and provides two-dimensional images of the subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (quantum dots, Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of the subcellular localization of pituitary hormone and its mRNA. Both EM-ISH&IHC and ISH & IHC using Qdots and CLSM are useful for understanding the relationships between protein and mRNA simultaneously in two or three dimensions. CLSM observation of rab3B and SNARE proteins such as SNAP-25 and syntaxin has revealed that both rab3B and SNARE system proteins play important roles and work together as the exocytotic machinery in anterior pituitary cells. Another important issue is the intracellular transport and secretion of pituitary hormone. We have developed an experimental pituitary cell line, GH3 cell, which has growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes GH linked to EYFP upon stimulation by Ca²+ influx or Ca²+ release from storage. This GH3 cell line is useful for the real-time visualization of the intracellular transport and secretion of GH. These three methods from conventional immunohistochemistry and fluorescein imaging allow us to consecutively visualize the process of transcription, translation, transport and secretion of anterior pituitary hormone.
Molecules 01/2011; 16(5):3618-35. · 2.39 Impact Factor
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Tomoaki Toyone,
Tomoyuki Ozawa,
Koya Kamikawa,
Atsuya Watanabe,
Keisuke Matsuki,
Takeshi Yamashita,
Ryutaro Shiboi, Masato Takeuchi,
Yuichi Wada,
Kunimasa Inada,
Yasuchika Aoki,
Gen Inoue,
Seiji Ohtori,
Tadashi Tanaka
[show abstract]
[hide abstract]
ABSTRACT: Case-control study.
To assess the long-term prevalence of vertebral fractures after lumbar spinal fusion with instrumentation.
The incidence of the adjacent and the nonadjacent, remote level subsequent vertebral fractures after lumbar spinal fusion is not well described in the literature.
The study is a retrospective analysis of 100 consecutive patients of 55 years of age or older with spinal fusion for degenerative diseases between L1 and S1, and instrumentation for less than 4 segments. Patients with prevalent vertebral fractures defined at the time of surgery, or patients with secondary causes of osteoporosis were excluded. Mean follow-up period was 10.2 years (range, 7-14 years). Acute vertebral fractures were determined by magnetic resonance imaging and lateral spine radiographs.
Acute vertebral fractures were determined in 20 vertebrae in 14 (24%) of the 59 female patients, whereas 1 male patient (2%) had 1 vertebral fracture during the follow-up period. Eighteen of the 21 fractures occurred within 2 years of the spinal instrumentation surgery. Regarding time to fracture occurrence after surgery, adjacent level fractures occurred within 8 months, and remote level fractures occurred between 8 and 22 months after surgery.
Postmenopausal female patients who underwent lumbar spinal instrumentation surgery were susceptible to develop subsequent vertebral fractures within 2 years after surgery. The greater the number of spinal segments between the fracture and the instrumentation was, the longer the time after surgery.
Spine 10/2010; 35(21):1915-8. · 2.08 Impact Factor
