Publications (2)6.9 Total impact
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Article: Silver-mediated exo-selective tandem desilylative bromination/oxycyclization of silyl-protected alkynes: synthesis of 2-bromomethylene-tetrahydrofuran.
Chemistry - An Asian Journal 04/2011; 6(8):1943-7. · 4.50 Impact Factor -
Article: Hs.137007 is a novel epigenetic marker hypermethylated and up-regulated in breast cancer.
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ABSTRACT: This study was conducted to mine novel breast-specific unigenes and analyze their epigenetic regulation in breast cancer. Differential digital display and methylation analysis identified the Hs.137007 gene containing a Kelch domain as a candidate novel epigenetic marker. In 50 pairs of breast cancer tissues and nearby normal tissues the methylation level of the 14 CpG sites at the promoter region (-778 to -485) of the gene was higher in cancer tissues (72-93%) than in normal tissues (31-83%), with a high correlation rate (p<0.05). End-point RT-PCR and real-time RT-PCR revealed that Hs.137007 was up-regulated in cancer tissues. A clear relationship between high methylation levels and up-regulated expression was also observed in the cultured breast cell lines. The MCF7 (90-100%) and MDAMB468 (100%) cancer cell lines that showed higher methylation than the BT549 (20-90%) and 184B5 (10-100%) at the 14 CpGs also showed elevated gene expression. Taken together, these results indicate that the Hs.137007 gene is a novel gene specifically expressed in the breast that can be utilized as an epigenetic marker of breast cancer.International Journal of Oncology 05/2010; 36(5):1105-11. · 2.40 Impact Factor
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Institutions
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2011
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Seoul National University
- College of Pharmacy
Seoul, Seoul, South Korea
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