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Publications (5)6.51 Total impact

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    ABSTRACT: Fibronectin 1 (FN1) is a glycoprotein that is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defenses and metastasis. The aim of this study was to elucidate the FN1 protein expression in renal cell carcinoma (RCC) and to determine its potential prognostic relevance. A total of 270 clear cell RCC tissue specimens were collected from patients undergoing surgery for renal tumors. Biomarker expression was determined by immunohistochemistry and correlated with clinical variables. Survival analysis was carried out for 153 patients with complete follow-up data and pathologically proven clear cell carcinoma of the kidney. The follow-up group had a mean follow-up period of 83.8 months (IQR 26.2-136.2 months). The calculated median 5-year overall and tumor-specific survival rate of all 153 evaluable patients was 66.6 and 71.0%, respectively. A higher disease-related mortality rate was observed among patients with cytoplasmic FN1 expression (41.3 vs. 24.7%, p=0.039, Fisher's exact test). No significant correlation was found between FN1 staining and patient characteristics such as age, gender, tumor differentiation and visceral metastasis. However, there was a trend for FN1 expression and correlation with tumor stage and lymph node metastasis (p=0.085 and p=0.203; respectively). The Kaplan-Meier analysis revealed significant differences in the 5-year tumor-specific survival for patients with and without cytoplasmic FN1 expression (64.8 vs. 77.7%; p=0.035, log-rank test). However, results of the multivariate Cox regression analysis showed that FN1 expression was not an independent marker of either overall or tumor-specific survival. In conclusion, FN1 protein expression in RCC is associated with a higher disease-related mortality rate, indicating a possible role in RCC progression. Therefore, our data on FN1 encourage further investigations to determine the role of FN1 in RCC.
    Oncology letters 04/2012; 3(4):787-790. · 0.24 Impact Factor
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    ABSTRACT: Caveolae play a significant role in disease phenotypes such as cancer, diabetes, bladder dysfunction, and muscular dystrophy. The aim of this study was to elucidate the caveolin-1 (CAV1) protein expression in renal cell cancer (RCC) and to determine its potential prognostic relevance. 289 clear cell RCC tissue specimens were collected from patients undergoing surgery for renal tumors. Both cytoplasmic and membranous CAV1 expression were determined by immunohistochemistry and correlated with clinical variables. Survival analysis was carried out for 169 evaluable patients with a median follow up of 80.5 months (interquartile range (IQR), 24.5-131.7 months). A high CAV1 expression in the tumor cell cytoplasm was significantly associated with male sex (p = 0.04), a positive nodal status (p = 0.04), and poor tumor differentiation (p = 0.04). In contrast, a higher than average (i.e. > median) CAV1 expression in tumor cell membranes was only linked to male sex (p = 0.03). Kaplan-Meier analysis disclosed significant differences in 5-year overall (51.4 vs. 75.2%, p = 0.001) and tumor specific survival (55.3 vs. 80.1%, p = 0.001) for patients with higher and lower than average cytoplasmic CAV1 expression levels, respectively. Applying multivariable Cox regression analysis a high CAV1 protein expression level in the tumor cell cytoplasm could be identified as an independent poor prognostic marker of both overall (p = 0.02) and tumor specific survival (p = 0.03) in clear cell RCC patients. Over expression of caveolin-1 in the tumour cell cytoplasm predicts a poor prognosis of patients with clear cell RCC. CAV1 is likely to be a useful prognostic marker and may play an important role in tumour progression. Therefore, our data encourage further investigations to enlighten the role of CAV1 and its function as diagnostic and prognostic marker in serum and/or urine of RCC patients.
    BMC Urology 12/2011; 11:25. · 1.69 Impact Factor
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    ABSTRACT: Caveolae play a significant role in disease phenotypes, such as cancer, diabetes, bladder dysfunction and muscular dystrophy. The aim of this study was to elucidate the expression of caveolin (CAV)1 in the development of renal cell cancer (RCC) and to determine a possible prognostic relevance for optimal clinical management. 109 RCC and 81 corresponding normal tissue specimens from the same kidney were collected from patients undergoing surgery for renal tumors and subjected to total RNA extraction. Quantification of CAV1 mRNA expression was performed using real-time reverse transcription PCR with three endogenous controls for renal proximal tubular epithelial cells and the ΔΔCt method for calculation of relative quantities. Expression levels were correlated to clinical variables. Tissue-specific mean CAV1 expression was significantly increased in RCC compared with normal renal tissue (p = 0.0003; paired Wilcoxon rank sum test). CAV1 expression was increased 1.9-fold in clear cell RCC compared with papillary RCC (p = 1.48 × 10(-7); unpaired Wilcoxon rank sum test). Patients with advanced disease had higher CAV1 expression when compared with organ-confined disease (p = 0.019; unpaired Wilcoxon rank sum test). Moreover, mean tissue-specific CAV1 expression was increased in patients with distant metastasis at the time of diagnosis compared with patients without metastasis (p = 0.0058; unpaired Wilcoxon rank sum test). To our knowledge, this is the first study to show that CAV1 mRNA expression, using quantitative real-time PCR, is significantly higher in RCC compared with normal renal tissue and increases with tumor stage. CAV1 mRNA expression might serve as a candidate biomarker for objective prognosis indicating RCC aggressiveness. Our data encourage further investigations to determine the role of CAV1 in RCC.
    Biomarkers in Medicine 04/2011; 5(2):219-25. · 3.22 Impact Factor
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    ABSTRACT: In contrast to anterior shoulder instability there seems to be no single key lesion in posterior shoulder instability. Therefore, the purpose of this study was to determine the biomechanical effect of specific posterior capsulolabral lesions. Our hypothesis was that a posterior capsule lesion will have a predominant effect compared to a labrum detachment (Bankart lesion). Stability testing of 16 cadaveric human shoulders was performed. The specimens were distributed to two groups: the labrum lesion group and the capsular lesion group. In the labrum lesion group three different conditions were tested consecutively: posteroinferior Bankart lesion, additive pHAGL lesion, additive posterosuperior Bankart lesion. In the capsular lesion group two conditions were tested: posteroinferior capsule cut including a glenoidal transection of the pIGHL, additive rotator interval and superior capsule lesion (SGHL and CHL cut). All lesions were set arthroscopically. Biomechanical testing was performed in two positions: the sulcus-test position and the jerk-test position each with a passive humerus load of 50 N in the posterior, posteroinferior and inferior direction. A posteroinferior Bankart lesion resulted in a percentage increase of 86% posterior translation in the jerk position and an increase of 31% inferior translation in the sulcus position. An additional pHAGL lesion resulted in a significant increase of posterior and inferior translation given by 31 and 41% in the jerk position. Regarding the capsular lesions, a cut of the posteroinferior capsule and the pIGHL resulted in a significant increased inferior translation of 53% in the sulcus position but did not cause a significant increase of posterior translation in the jerk position. If an additional rotator interval lesion is set the inferior translation is again significantly increased. On the basis of our results traumatic posterior shoulder instability must be suspected to be bidirectional posteroinferior independently if a posterior capsule lesion or a posterior Bankart lesion is evident. Capsular and labral lesions both have a significant biomechanical effect but differ in the predominant direction of instability, which is posterior for the Bankart lesion and inferior for the capsular lesion. An additional pHAGL or rotator interval lesion aggravates the posteroinferior instability and must be respected in the surgical treatment strategy.
    Archives of Orthopaedic and Trauma Surgery 03/2011; 131(3):421-7. · 1.36 Impact Factor
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    ABSTRACT: Fragestellung Die undirektionale posteriore Schulterinstabilität ist primär traumatisch bedingt. Die operative Therapie adressiert entweder den dorsalen Kapsel-Bandapparat oder das knöcherne Glenoid. In der vorliegenden Studie wurden ein arthroskopischer posteriorer Bankart-Repair mit einem offenem Standardverfahren (extraartikulärer Knochenblock plus T-Kapselshift) auf ihre biomechanische Effektivität hin verglichen. Methoden Es wurden 16 humane Schulterpräparate mittels Roboter/KMS (Kraft-Moment-Sensor)-Apparatur auf posteriore und inferiore Translation sowie bezüglich der maximalen Innenrotation untersucht. Zunächst wurden die intakten Schultern im nativen und „ventilierten“ Zustand getestet. Anschließend wurde arthroskopisch ein posteriorer Bankart-Defekt simuliert (6:00 Position bis zum Bizepssehnenansatz). Jeweils acht Schultern wurden dann in arthroskopischer und offener Technik operiert. Der arthroskopische Bankart-Repair erfolgte mit drei Titan-Nahtankern (3,5 mm) in 7:00, 9:00 und 11:00 Position. Die offene Rekonstruktion beinhaltete einen glenoidbasigen T-Shift der dorsalen Kapsel und die extrakapsuläre Anlagerung eines trikortikalen Knochenblocks. Der Knochenblock wurde mit zwei kanülierten 4,5 mm Schrauben fixiert und überragte des Glenoid lateral um 10 mm und das Labrum um ca. 5 mm. Die Translationstestung erfolgte mit einer Provokationskraft von 50 N in posteriore, posterio-inferiore und inferiore Richtung. Getestet wurde in 0° Grad und 60° glenohumeraler Abduktion/90° Horizontaladduktion (Jerk-Position). Die Statistik erfolgte mittels T-Test für unverbundene Stichproben (p<0,05). Ergebnisse Die posteriore Bankart-Läsion führte zu eine signifikanten Steigerung sowohl der posterioren als auch der inferioren Translation (posterior: 13,2 mm in 0°-Abduktion und 10,8 mm in Jerk-Position, inferior: 12,0 mm in 0°-Abduktion). Die offene Technik mit Knochenblockanlagerung führt zu einer signifikanten Reduktion lediglich der posterioren Translation während die inferiore Instabilität persistiert (posterior 5,6 mm in 0°-Abduktion und 4,9 mm in 60°-Abduktion, inferiore Translation 9,4 mm). Der arthroskopische Bankart-Repair reduziert sowohl die inferiore als auch die posteriore Tranlation auf das Niveau des ventilierten Zustandes (posterior: 6,6 mm in 0°-Abduktion und 5,9 mm in 60°-Abduktion). Die maximale Innenrotation wird durch das Verfahren um 15° reduziert (59° versus 74°). Diskussion Beide Verfahren reduzieren die durch den Bankart-Defekt pathologisch erhöhte posteriore Translation auf das Niveau des intakten bzw. ventilierten Gelenkes. Dabei wirkt die offene Knochenblock-Technik trotz des Kapselshiftes primär unidirektional posterior. Der arthroskopische Bankart-Repair ermöglicht zudem die Adressierung einer assoziierten inferioren Instabilität.
    Sport-Orthopädie - Sport-Traumatologie - Sports Orthopaedics and Traumatology. 26(2).