Publications (2)2.69 Total impact
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Article: Effect of Canola Protein Extraction Parameters on Protein-Based Plastic Properties
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ABSTRACT: Canola meal protein isolates (CMPI) were prepared using defatted canola meal flour by varying the pH values used in alkaline solubilization and acid precipitation steps. Protein-based plastics were prepared using CMPI and tested for mechanical and water resistance properties. Statistical models showed that tensile strength and toughness of plastics were affected by both solubilization and precipitation pH of protein isolates, whereas elongation and water absorption were impacted only by solubilization pH. Mechanical properties showed strong positive correlation with each other while water absorption showed strong negative correlation with mechanical properties. The response surfaces for the mechanical properties were notably different from that of protein yield demonstrating the trade-off between optimizing protein yields and resultant mechanical properties of protein-based plastics.Journal of Biobased Materials and Bioenergy 11/2011; 5(4):500-506. · 1.04 Impact Factor -
Article: Effects of protein separation conditions on the functional and thermal properties of canola protein isolates.
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ABSTRACT: Canola meal protein isolates were prepared from defatted canola meal flour using alkaline solubilization and acid precipitation. A central composite design was used to model 2nd-order response surfaces for the protein yield and the functional properties of protein isolates. The solubilization pH and precipitation pH were used as design factors. The models showed that the protein yield and functional properties of isolates, such as water absorption and fat absorption, were sensitive to both solubilization pH and precipitation pH, whereas the emulsification was sensitive to only solubilization pH. Gel electrophoresis analysis of protein fractions gave evidence to the compositional changes between proteins isolated under different conditions. Differences in glass transition temperatures suggest that proteins tend to be more denatured when solubilized at highly alkaline conditions. These conformational and compositional changes due to different protein separation conditions have contributed to the changes in functional properties of protein isolates. PRACTICAL APPLICATION: Protein isolation conditions may be determined primarily through optimization of total protein yield. Improvements in protein functional properties may be achieved with a relatively small sacrifice in yield by altering isolation conditions.Journal of Food Science 04/2011; 76(3):E266-73. · 1.66 Impact Factor
