Andrey D Kukhlevskiy

Russian Academy of Sciences, Moskva, Moscow, Russia

Are you Andrey D Kukhlevskiy?

Claim your profile

Publications (4)7.78 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A facultatively anaerobic, Gram-stain negative, rod-shaped and yellow pigmented bacterium, designated strain IDSW-73(T), was isolated from a seawater sample and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain formed a distinct phyletic line in the family Flavobacteriaceae and is most closely related to the members of the genus Leeuwenhoekiella, with 16S rRNA gene sequence similarity of 91.4-92.6 %. Strain IDSW-73(T) was found to be able to grow with 0-12 % NaCl and at 4-33 °C; and was able to hydrolyse gelatin, starch and Tweens 20, 40 and 80. The DNA G+C content was determined to be 42.2 mol%. The predominant cellular fatty acids were identified as branched-chain saturated and unsaturated and straight-chain unsaturated fatty acids such as iso-C15:0, iso-C15:1, iso-C17:1 ω9c, C15:1 ω6c, iso-C15:0 3-OH, iso-C17:0 3-OH and summed feature 3 (as defined by MIDI), comprising iso-C15:0 2-OH and/or C16:1 ω7c. The polar lipids found were phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The major respiratory quinone was identified as MK-6. The significant molecular distinctiveness between the novel isolate and its nearest neighbours were strongly supported by notable differences in physiological and biochemical tests. Therefore, strain IDSW-73(T) is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Flavimarina pacifica gen. nov., sp. nov. is proposed. The type strain is IDSW-73(T) (=KCTC 32466(T) = KMM 6759(T)). Emended descriptions of the recognized species of the genus Leeuwenhoekiella are also proposed.
    Antonie van Leeuwenhoek 06/2014; · 2.07 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A strictly aerobic, Gram-stain-negative, rod-shaped and red-orange pigmented bacterium, designated strain KMM 6395(T), was isolated from the green alga Cladophora stimpsoni and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Hyphomonadaceae of the class Alphaproteobacteria being most closely related to the type strain of the single species of the genus Litorimonas, Litorimonas taeanensis G5(T), with 16S rRNA gene sequence similarity of 96.8 %. Strain KMM 6395(T) grew with 1-5 % NaCl and at 4-35 °C, hydrolysed starch and Tween 80. The DNA G+C content was 48.7 mol%. The prevalent fatty acids were C18:1 ω7c, C19:1 ω8c and C18:1 ω7c 10-methyl. The polar lipid profile was characterized by the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and an unidentified glycolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour, L. taeanensis G5(T), were strongly supported by the differences in physiological and biochemical tests. Therefore, strain KMM 6395(T) represents a novel species of the genus Litorimonas, for which the name Litorimonas cladophorae sp. nov. is proposed. The type strain is KMM 6395(T) (=KCTC 23968(T) = LMG 26985(T)). The emended descriptions of the genus Litorimonas and L. taeaensis are also provided.
    Antonie van Leeuwenhoek 03/2013; · 2.07 Impact Factor
  • Olga I Nedashkovskaya, Andrey D Kukhlevskiy, Natalia V Zhukova
    [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 6Alg 8(T), was isolated from the common Pacific green alga Ulva fenestrata. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 97.6 % to Polaribacter dokdonensis DSW-5(T) and 92.8-96.1 % to other recognized Polaribacter species. The prevalent fatty acids of strain 6Alg 8(T) were iso-C(15:0), iso-C(15:1), iso-C(15:0) 2-OH, C(15:0) and C(15:1)ω6. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The DNA G+C content of the type strain is 31.6 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Polaribacter, for which the name Polaribacter reichenbachii sp. nov. is proposed. The type strain is 6Alg 8(T) (= KCTC 23969(T) = KMM 6386(T) = LMG 26443(T)).
    Current Microbiology 09/2012; · 1.52 Impact Factor
  • Olga I Nedashkovskaya, Andrey D Kukhlevskiy, Natalia V Zhukova
    [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-negative, facultatively anaerobic, slightly halophilic bacterium that was motile by gliding, designated KMM 6390(T), was isolated from the coastal green alga Ulva fenestrata collected from the Sea of Japan. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6390(T) was a member of the genus Winogradskyella, with Winogradskyella echinorum KMM 6211(T) as its closest relative (97.8% 16S rRNA gene sequence similarity). 16S rRNA gene sequence similarities between the isolate and other members of the genus Winogradskyella were 95.4-96.7%. DNA-DNA relatedness between the isolate and W. echinorum KMM 6211(T) was 27%. Strain KMM 6390(T) was able to grow with 0.5-4.0% NaCl and at 4-37 °C. The isolate decomposed casein, gelatin, starch and DNA and produced acid from galactose, D-glucose, lactose and maltose. The major fatty acids were iso-C(15:0), iso-C(15:1), anteiso-C(15:0) and C(15:0). The polar lipid profile comprised phosphatidylethanolamine and two unknown aminolipids. The DNA G+C content was 34.2 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic investigations, strain KMM 6390(T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella ulvae sp. nov. is proposed; the type strain is KMM 6390(T) (=KCTC 23626(T)=LMG 26444(T)). Emended descriptions of the genus Winogradskyella and of W. thalassocola, W. echinorum, W. exilis and W. eximia are also provided.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 62(Pt 7):1450-6. · 2.11 Impact Factor