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Publications (4)9.99 Total impact

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    ABSTRACT: The aim of this work is to investigate the biocompatibility and staining properties of DSS: 3,3'-Di-(4-sulfobutyl)-1,1,1',1'-tetramethyl-di-1H-benz[e]indocarbocyanine (DSS). Dye concentrations of 0.5, 0.25, and 0.1% were evaluated (290 and 295 mOsm). Toxicity was assessed using a colorimetric test measuring the inhibition of ARPE 19 cell, human primary RPE cell, and human Müller cell proliferation. Exposure time was 30, 60, 120, and 300 s. Indocyanine green (ICG) (0.5, 0.25, and 0.1%) served as a control. Cells were also illuminated with plain white light (750 mW/cm(2)) for 10 min to assess phototoxic effects. Besides staining of porcine and human lens capsule, internal limiting membrane (ILM)-staining was assessed by applying 0.25 and 0.5% DSS over the macula in two human post-mortem eyes. DSS of 0.25 and 0.1% showed no toxic effect on primary RPE cells and MIO-M1cells, and 0.5, 0.25, and 0.1% for ARPE-19 cells. In MIO-M1cells, 0.5% dye showed a significant reduction of mitochondrial dehydrogenase activity only following an exposure time of 300 s. Following illumination, ICG showed a significantly more pronounced effect on cell viability in primary RPE cells and MIO-M1cells compared to DSS. The absorption maximum is found at 591 nm; the even more bathochromic fluorescence proceeds with a common Stokes' shift where maxima at 620 and 660 nm with a quantum yield of 32% were found. The fluorescence is sufficiently hypsochromic and the fluorescence quantum yield high enough for an easy visual detection. The contrast and staining properties at the ILM were excellent and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted. Our results indicate that this new cyanine dye DSS may represent an alternative for ILM staining due to its matched absorption concerning visibility and fluorescence qualities as well as its good biocompatibility.
    Albrecht von Graæes Archiv für Ophthalmologie 02/2012; 250(6):829-38. · 1.93 Impact Factor
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    ABSTRACT: Purpose To investigate the biocompatibility and staining properties of a new cyanine dye (DSS: 3,3´-Di-(4-sulfobutyl)-1,1,1´,1´-tetramethyl-di-1H-benz[e]indocarbocyanine).Methods Dye concentrations of 0.5%, 0.25% and 0.1% were evaluated (osmolarity between 290 and 295 mOsm). Toxicity was assessed using a colorimetric test measuring the inhibition of ARPE 19 cell, human primary RPE cell and human Müller cell proliferation. Exposure time was 30, 60, 120 and 300 seconds. Indocyanine green (ICG) (0.5%, 0.25% and 0.1%) served as a control. Besides staining of porcine and human lens capsule, internal limiting membrane (ILM)-staining was assessed by applying 0.25% and 0.5% DSS over the macula in two human post-mortem eyes.Results The dye DSS did not reveal any toxicity on ARPE-19, primary human RPE cell and human Müller cells proliferation in all concentrations and exposure times investigated. The absorption maximum is found at 591 nm, the even more bathochromic fluorescence proceeds with a common Stokes’ shift where maxima at 620 and 660 nm with a quantum yield of 32% were found. The fluorescence is sufficiently hypsochromic and the fluorescence quantum yield high enough for an easy visual detection. The contrast and staining properties at the ILM was excellent because of matched optical properties and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted.Conclusion Our results indicate that this new cyanine dye DSS may represent an alternative for ILM staining due to its matched absorption concerning visibility and fluorescence qualities as well as its good biocompatibility. The dye is superior compared with ICG where there is no matching of the UV/Vis spectra.
    Acta ophthalmologica 09/2011; 89(s248). · 2.44 Impact Factor
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    ABSTRACT: Cyanine dyes were prepared as optical contrast media for supporting the surgery of the lamina limitans interna (LLI) of the retina and other structures of the human eye. Their absorption spectra were adapted both to the spectral sensitivity of the human eye and to standard illumination. The contrast could be further amplified by the application of the strong fluorescence of the dyes used. The binding of the dyes to various surfaces was studied. No toxic effects could be detected for the applied dyes.
    Journal of Medicinal Chemistry 06/2011; 54(11):3903-25. · 5.61 Impact Factor
  • Ana Varja