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Publications (8)7.06 Total impact

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    ABSTRACT: Twelve half-sib groups and 36 full-sib groups of small abalone Haliotis diversicolor were obtained by the unbalanced nest design using artificial fertilization of 3 females by each male. Heritabilities of growth-related traits were estimated from postlarva to market size at days 10, 40, 120, 220, 320, and 420. The estimated heritabilities based on sire components for shell length and shell width were 0.15-0.37 and 0.18-0.42, respectively. Heritability estimate for shell length and shell width based on dam component were larger than those based on sire component. The results in the current study indicate that genetic improvement through selective breeding conducted on small abalone might be available and would be an efficient method to obtain a positive response to selection.
    Journal of Shellfish Research 11/2010; · 0.87 Impact Factor
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    ABSTRACT: A total of 241 samples of small abalone (Nation's diversicolor) from 8 full-sib families of market size were used in this experiment. Shell length (X-1), shell width (X-2), shell height (X-3), apex height (X-4), body weight (Y-1), muscle weight (Y-2), and shell weight (Y-3) were measured, and the correlation coefficient matrix was calculated. The shell shape traits were used as independent variables, then body weight and muscle weight were used as dependent variable for path analysis. Path coefficients, determination coefficients, and correlation index were calculated. The results showed that correlation coefficients between each shell shape trait and body weight, muscle weight, and shell weight were all significant (P < 0.01). For the 4 shell morphological traits, body weight (Y-1) had the highest correlation coefficient with shell length (X-1), and muscle weight (Y-2) had the highest correlation coefficient with shell width (X-2). The results of high correlation index would be useful for selecting important growth-related traits in genetic breeding program of small abalone.
    Journal of Shellfish Research 11/2010; · 0.87 Impact Factor
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    ABSTRACT: Interspecific hybrid families of female Haliotis diversicolor X male H. discus discus were produced and analyzed using amplified fragment length polymorphism (AFLP) technology to reveal the genetic makeup of F1 progenies. The survival rates of the hybrid F1 were very low, ranging from 0-0.13%. Twenty hybrid F1 from 3 families along with 3 different female parents and their common male parent were analyzed with 3 AFLP primer combinations. In total, 266 markers were detected. Genetic relationships among the progenies and the parents were evaluated by generating a similarity and genetic distance matrix. The genetic divergence between Haliotis diversicolor and Haliotis discus was at a high level, with genetic distance ranging from 1.471-1.492. The AFLP band patterns of hybrid F1 progeny were similar to those of the female parents, but were quite different from that of the male parent. The mean genetic distance between hybrid F1 and their female parents were 0.024-0.039, slightly less than that among the female parents, which indicates that the hybrid F1 shared high genetic similarity with their female parents, Haliotis diversicolor. However, 0-0.8% of total AFLP bands of each individual were found to be parental bands, and 0-3.3% were found to be nonparental bands. The possible reason for the presence of paternal-specific and nonparental bands is discussed.
    Journal of Shellfish Research 11/2010; · 0.87 Impact Factor
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    ABSTRACT: Amplified fragment length polymorphism (AFLP) analysis of the population genetic structure and genetic diversity of Haliotis discus hannai (D), Haliotis gigantea (G), and their reciprocal hybrids D female x G male (DG) and G female x D male (GD) was carried out in this study. A total of 479 unambiguous and highly repeatable AFLP markers, 311 of which (64.93%) were polymorphic, were obtained using 7 primer combinations. The reciprocal hybrids inherited bands from both parents, indicating that the hybrids were truly heterogeneous. The Shannon diversity index for D, G, and their reciprocal hybrid populations DG and GD was 0.169 +/- 0.188, 0.211 +/- 0.227, 0.236 +/- 0.267, and 0.231 +/- 0.242, respectively. Analysis of molecular variance revealed that 29.58% of the variance was among populations, whereas 71.42% of variance was within populations. Genetic distance was maximum (0.681) between D and G, and was minimum (0.482) between GD and G. The 4 populations were clustered into 2 major clades using the unweighted pair group method with arithmetic mean. All genetic parameters indicated that there was plentiful genetic diversity in the reciprocal hybrids of D X G. Results of this study suggest that these AFLP markers can be used in the future to enhance current breeding practices in abalone culture because of the large numbers of polymorphic markers.
    Journal of Shellfish Research 11/2010; · 0.87 Impact Factor
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    ABSTRACT: Interspecific hybrids between Haliotis discus hannai Ino and Haliotis gigantea Gmelin were produced in this study. The hybridity of the interspecific hybrids was confirmed by using the methods of amplified fragment-length polymorphism (AFLP) and microsatellite [simple sequence repeats (SSR)] markers. Five AFLP primer combinations were used to develop the AFLP profiles of H. discus hannai, H. gigantea and their reciprocal hybrids. AFLP analysis revealed that genetic variations of H. discus hannai and H. gigantea were relatively diverse and each species holds species-specific bands. The AFLP profiles of reciprocal hybrids showed that all of the hybrids inherited bands specific to H. discus hannai and H. gigantea. Of a total of 20 microsatellite loci, which were selected from H. discus hannai microsatellite markers evaluated, eight loci were polymorphic in H. gigantea samples, with an average of 3.375 alleles per locus. Preliminary screening showed that, two of these eight microsatellite loci (Awb002 and Awb022) could be used as species-specific markers to distinguish the hybrids and their parental species. Simple sequence repeats analysis showed that the reciprocal hybrids inherited one allele from each parent for both of the two SSR loci investigated. These data strongly suggest that the induced interspecific hybrid is a true hybrid between H. discus hannai and H. gigantea. Science and Technology Project of Fujian Province [2008N0042]; Hi-Tech Research and Development (863) Program of China [2006AA10A407]
    Aquaculture Research 01/2010; · 1.42 Impact Factor
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    ABSTRACT: The enzyme ornithine decarboxylase (ODC) is the first limiting enzyme in polyamine biosynthesis and plays an important role in biological processes. Although recognized as a potential biomarker in vertebrates, it has not been well studied in aquatic animals. Identification of ODC is a prerequisite for its application as a selective breeding marker in small abalone, Haliotis diversicolor. In this article, we focused on the in vitro characterization of the ODC gene from small abalone. The expressed product showed decarboxylase activity for l-ornithine and was inhibited by DL-alpha-difluoromethylornithine hydrochloride hydrate. ODC was expressed in a wide range of tissues at varying levels, with the lowest in the hepatopancreas and the highest in the antennae. ODC is potentially useful as a molecular marker for small abalone breeding and can also facilitate further investigations on its biological functions in mollusks. National High Technology Research and Development Program of China [2006AA10A407]; Modern Agro-industry Technology Research System of China [nycytx-47]; Innovative Platform for Science and Technology, Fujian Province of China [2008N2004]
    Journal of the World Aquaculture Society 01/2010; · 0.75 Impact Factor
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    ABSTRACT: To determine whether genetic improvement can be attained through a selective breeding programme, divergent selection for shell length was applied to two stocks of Haliotis diversicolor. Stock A was descended from the cross between males from a Japanese wild population and females from a Taiwan aquacultured population and Stock B was from the Taiwan cultured population, which had been successively cultured in mainland China for about 10 generations. The 10% largest and 10% smallest abalones for each of these two stocks were selected as parents for the large-selected and small-selected lines respectively. Equal numbers of abalone were randomly chosen from the two stocks to serve as parents for the control lines before the selection. The selected and control lines were reared under the same conditions at early juvenile, later juvenile and grow-out stages. Stock A showed a significantly higher response to selection and realized heritability than Stock B (P < 0.01). The large-selected line of Stock A and Stock B grew 12.79% and 4.58% faster than their control lines on shell length respectively. The average realized heritability for shell length was 0.441 +/- 0.064 for Stock A and 0.113 +/- 0.013 for Stock B. Responses to selection were different at different ages in each stock and the body weights of the selected lines were significantly different from the control lines in both stocks at the grow-out stage. Asymmetric responses to selection in the two directions were also observed in both stocks. Differences in response to selection and realized heritability between the two stocks are presumably due to genetic variability. Hi-Tech Research and Development (863) Program of China [2006AA10A407]
    Aquaculture Research 01/2009; · 1.42 Impact Factor
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    ABSTRACT: In this study, effects of sperm concentration and gamete age on fertilization success of Haliotis discus hannai (D) X H. gigantea (G) were investigated. Results showed that the fertilization rates of heterologous crosses H. discus hannai female x H. gigantea male (DG) and H. gigantec female X H. discus hannai male (GD) were consistently lower than those of homospecific groups H. discus hannai female x H. discus hannai male (DD) and H. gigame female X H. gigantea male (GG). In sperm concentration experiments, the sperm concentrations that yielded maximum fertilization rates with the least abnormality in subsequent development were 4.66 X 10(7) sperm/mL for the DG cross and 2.6 x 10(7) sperm/mL for the reciprocal cross GD. In gamete age experiments, the optimal fertilization rates were achieved in heterologous crosses when freshly spawned ova were fertilized with sperm that were released within 0.5 h. Furthermore, in heterospecific crosses, when ova were fertilized 10 min after being spawned, fertilization rates declined significantly with increasing ova age. It is suggested to use fresh gametes and higher sperm concentrations for hybridization between H. discus hannai and H. gigantea.