Yuncong Chen

Nanjing University, Nan-ching, Jiangsu Sheng, China

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Publications (14)97.3 Total impact

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    ABSTRACT: The homeostasis of mitochondrial pH (pHm) is crucial for cell physiology. Developing small molecular fluorescent sensor for the ratiometric detection of pHm fluctuation is highly demanded yet challenging. A ratiometric pH sensor, Mito-pH, was constructed by integrating a pH sensitive FITC fluorophore with a pH insensitive hemicyanine group. The hemicyanine group also acts as the mitochondria targeting group due to its lipophilic cationic nature. Besides its positive mitochondria-targetability, this sensor provides two ratiometric pH sensing modes, the dual excitation/dual emission mode (Dex/Dem) and dual excitation (Dex) mode, and its linear and reversible ratiometric response range from pH 6.15 to 8.38 makes this sensor suitable for the practical tracking of pHm fluctuation in live cells. With this sensor, the stimulated pHm fluctuation has been successfully tracked in a ratiometric manner via both fluorescence imaging and flow cytometry.
    Chemical Science 03/2015; DOI:10.1039/C4SC04021J · 8.60 Impact Factor
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    ABSTRACT: Zn(2+) plays essential roles in various physiological processes in living systems, and the investigation of Zn(2+) related physiology and pathology has attracted considerable interest. Because photoluminescence (PL) imaging possesses distinct advantages, such as high sensitivity and non-invasiveness, and excellent temporal and spatial resolution, it has become a powerful tool for the real time monitoring of Zn(2+) distribution, uptake, and trafficking. Over the last two decades, great efforts have been devoted to PL Zn(2+) imaging in living systems, which proved the Zn(2+) fluctuations in physiological processes and the temporal-spatial distribution of labile Zn(2+) as well as the localization of labile Zn(2+) pools. Advances in PL techniques, such as fluorescence microscopy, confocal fluorescence microscopy, two photon fluorescence microscopy, lifetime based techniques and luminescence optical imaging systems, have made remarkable contributions in tackling major challenges in Zn(2+) PL imaging. With the rational design and proper use of fluorescent sensors, Zn(2+) imaging in various cell lines, organelles, tissues, organs and living animals has been realized, which was shown to be crucial in elucidating the biological and physiological roles of labile Zn(2+).
    Chemical Society Reviews 03/2015; DOI:10.1039/c5cs00005j · 30.43 Impact Factor
  • 01/2014; 44(4):631. DOI:10.1360/032013-319
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    ABSTRACT: A visible light excitable ratiometric Zn(2+) sensor was developed by integrating a Zn(2+) chelator as the ICT donor of the fluorophore sulfamoylbenzoxadiazole, which displays the Zn(2+)-induced hypsochromic emission shift (40 nm) and favors the in vivo ratiometric Zn(2+) imaging in zebrafish larvae.
    Chemical Communications 12/2013; 50(10). DOI:10.1039/c3cc46262e · 6.72 Impact Factor
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    ABSTRACT: ICT fluorophore benzoxadiazole with its electron-donating group modified as a Cu(2+) chelator was conjugated with coumarin to construct a new ratiometric sensor with reversible intracellular Cu(2+) imaging ability.
    Chemical Communications 07/2013; 49(69). DOI:10.1039/c3cc42959h · 6.72 Impact Factor
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    ABSTRACT: Quick: An exogenously induced quick increase of the H(2) S concentration (80 s) in MCF-7 cells can be visualized by ratiometric imaging using a new probe (CouMC) that can target mitochondria. CouMC was constructed by combining merocyanine and coumarin fluorophores. The selective nucleophilic addition of HS(-) to the merocyanine derivative at neutral pH is crucial for the rapid H(2) S detection.
    Angewandte Chemie International Edition 02/2013; 52(6). DOI:10.1002/anie.201207701 · 11.34 Impact Factor
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    ABSTRACT: A mitochondria-targeted fluorescent sensor (Mito-ST), constructed by integrating a sulfamoylbenzoxadiazole fluorophore with a phosphonium group, displays the specific Zn(2+)-induced hypsochromic shifts of both excitation (69 nm) and emission (35 nm) maxima. Its ratiometric Zn(2+) imaging ability via dual excitation mode has been applied in MCF-7 cells to clarify the different behaviours of mitochondrial Zn(2+) release stimulated by H(2)O(2) and SNOC.
    Chemical Communications 07/2012; 48(67):8365-7. DOI:10.1039/c2cc33648k · 6.72 Impact Factor
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    ABSTRACT: A hybrid coumarin-hemicyanine dye, Cou-BT, was developed as a new ratiometric and colorimetric sensor for cyanide with a sensing mechanism via nucleophilic addition of cyanide anion to the benzothiolium group. Cou-BT shows high sensitivity and selectivity for cyanide detection over other common anion species in aqueous acetonitrile solution. The calculated pseudo-first-order rate constant for cyanide anion addition was (2.13 ± 0.08) × 10(-2) s(-1) at 298 K, and the detection limit was estimated to be 0.64 μM. The DFT and TDDFT calculation results suggest that the ratiometric and colorimetric sensing behavior of Cou-BT upon its reaction with cyanide was due to the interrupted π-conjugation and blocked ICT progress.
    Organic & Biomolecular Chemistry 05/2012; 10(26):5073-6. DOI:10.1039/c2ob25516b · 3.49 Impact Factor
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    ABSTRACT: A novel sensitive and specific Hg(2+) chemodosimeter, derived from 1',3'-dithiane-substituted 2,1,3-benzoxadiazole, displays "turn-on" fluorescent and colorimetric responses via an Hg(2+)-triggered aldehyde recovery reaction. Its potential to monitor Hg(2+) in living organisms has been demonstrated using zebrafish larvae.
    Chemical Communications 04/2012; 48(42):5094-6. DOI:10.1039/c2cc31217d · 6.72 Impact Factor
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    ABSTRACT: The different DNA cleavage behaviors of 1,3- and 1,4-xylylene spaced bisCu(BPA)Cl2 complexes (BPA, bis(pyridyl-2-methyl)amine) display the positioning effect of xylylene spacers, implying the essential roles of spacers in the related cleavage (Y. Zhao et al., Chem.–Eur. J., 2006, 12, 6621–6629). Herein, a p-xylyl derivative (2,1,3-benzoxadiazole-4,7-dimethylene (BDDM)) was utilized as a bulky spacer to construct a new p-xylyl spaced bisCu(BPA)Cl2 complex 1 to explore the spacer's steric effect on the related DNA cleavage. Agarose gel electrophoresis demonstrated that complex 1 is able to convert the supercoiled plasmidpUC19DNA into the nicked DNA and linear DNA in the presence of excessive reductant. This DNA cleavage behavior is different from that of simple p-xylylene spaced Cu2(pTPXA)Cl4, which mediates only the nicked DNA and random DNA fragments. The DNA binding ability of complex 1 is higher than that of Cu2(pTPXA)Cl4, while its DNA nicking ability is lower than that of Cu(BPA)Cl2, as well as the dinuclear Cu2(pTPXA)Cl4, which displays a high DNA nicking efficiency due to the internuclear synergy in activating O2 to damage DNA. The linear DNA formation induced by complex 1 is realized via a non-random double-stranded scission process. The temporal profiles of the nicked DNA and linear DNA in the presence of 8 μM complex 1 imply that the two sequential neighboring breaks in the complementary strands lead to the formation of linear DNA. With the DNA cleavage behavior in the presence of different reactive oxygen species (ROS) quenchers, the DNA-bound hydroxyl radical was proposed as the main ROS involved in the cleavage. Structural optimization of this complex disclosed its larger intramolecular Cu–Cu distance than Cu2(pTPXA)Cl4, which may disfavor the internuclear synergic O2 activation. It was also proposed that the bulky BDDM spacer favors the two spaced copper centers function solely to damage in situ the two adjacent deoxyriboses from complementary strands to form the linear DNA, and the specific DNA binding mode of complex 1 due to the BDDM spacer disfavors the DNA-bound ROS to abstract a proton from deoxyribose and results in a lower DNA cleavage efficiency than mononuclear Cu(BPA)Cl2. The non-random linear DNA mediating ability and the decreased DNA cleavage efficiency caused by the bulky internuclear spacer shown in the current study provides a new alternative to regulate the DNA cleavage behavior of polycopper artificial nucleases via spacer modification.
    New Journal of Chemistry 02/2012; 36(3):644-649. DOI:10.1039/C1NJ20793H · 3.16 Impact Factor
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    ABSTRACT: A new visible light excitable Hg2+ sensor, NBD-TAEE, was prepared by bridging a 4-amino-7-nitro-benzoxadiazole (ANBD) fluorophore with thiaazacrown ethervia an ethylene spacer. Besides the specific Hg2+-induced absorption shift from 466 to 513 nm, this sensor exhibits the specific Hg2+-induced emission enhancement (29-fold) and fluorescent pH-independence from pH 6 to 13. Hg2+ addition even leads to the emission red shift from 532 to 570 nm, although NBD-TAEE is not a ratiometric fluorescent sensor due to its emission band being overlaid by the Hg2+-induced band. The instant and reversible “turn-on” Hg2+ response, fine cell permeability and cell imaging ability display its potential application in both environmental and Hg2+-polluted biological samples. Its analogue without the ethylene spacer, NBD-TAE, possesses the higher quantum yield and very poor Hg2+ binding ability. Their different emission behavior and sensing ability suggest that the spacer is quite essential for sensor construction.
    New Journal of Chemistry 03/2011; 35(3):607-613. DOI:10.1039/C0NJ00773K · 3.16 Impact Factor
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    ABSTRACT: The integration of bis(pyridin-2-ylmethyl)amine (BPA) with 8-sulfonamidoquinoline (SQ) resulted in a new fluorescent Zn2+ sensor of 1:1 binding stoichiometry. The synergic Zn2+ coordination of BPA and SQ motifs provides the sensor the advantage over TSQ and its analogues in discriminating mobile Zn2+ from the bound Zn2+ of unoccupied coordination sites in living systems. Its pH-independent Zn2+-enhanced emission in physiological condition and cell permeability make it an effective intracellular Zn2+ imaging agent. This sensor profits also from its confirmed Golgi-preferential affinity, and its pH-independent Zn2+ response in physiological pH range provides it the advantages over other xanthenone-based sensors.
    Inorganic Chemistry Communications 01/2011; 14(1):304-307. DOI:10.1016/j.inoche.2010.11.021 · 2.06 Impact Factor
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    ABSTRACT: Terpyridine/benzofurazan conjugation results in a new hybrid fluorophore of the colorimetric sensing ability for Fe(2+) and fluorimetric sensing ability for XII group cations. The improved emission properties and cell imaging ability imply it is a suitable platform to construct a fluorescent sensor for metal imaging in biological systems.
    Dalton Transactions 11/2010; 40(10):2173-6. DOI:10.1039/c0dt01053g · 4.10 Impact Factor
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    ABSTRACT: The DNA binding ability and binding mode of platinum complexes are crucial factors that govern their cytotoxic activity. In this work, circular dichroism spectroscopy, gel electrophoresis and MALDI-TOF MSspectrometry combined with enzymatic degradation have been used to elucidate the role of bridging ligands in DNA-binding ability and cross-linking patterns of two dinuclear antitumour active platinum(II) complexes, {[cis-Pt(NH3)2Cl]2L1}(NO3)2 (1, L1= 4,4′-methylenedianiline) and {[cis-Pt(NH3)2Cl]2L2}(NO3)2 (2, L2 = α,α′-diamino-p-xylene). Although both complexes have two cis-diammine-Pt(II) moieties (1,1/c,c), complex 1 exhibits much higher DNA-binding ability than complex 2. The former readily forms both 1,3- and 1,4-intrastrand cross-links with DNAoligonucleotides, while the latter preferentially forms 1,4- rather than 1,3-intrastrand cross-links. Cytotoxicity studies against a human non-small-cell lung cancer cell line (A549) demonstrate that complex 1 has higher activity than 2. These results show that the linker properties play a critical role in controlling the DNA-binding and cross-linking abilities and in modulating the cytotoxicity of dinuclear platinum complexes.
    Dalton Transactions 01/2009; DOI:10.1039/b913236h · 4.10 Impact Factor