Ying-Yi Chen

Publications (2)0.3 Total impact

• Article: Dr. Chen Ying-Yi's experience in treating premature ovarian failure.

  Hai-Mo Qian, Fan Xiao-Yan, Ying-Yi Chen
  Journal of Traditional Chinese Medicine 09/2010; 30(3):217-21. · 0.30 Impact Factor
• Article: 人工培養之蛹蟲草其水抽物降血糖能力之研究

  陳瑛宜, Ying-Yi Chen
  [show abstract] [hide abstract]
  ABSTRACT: 自古以來藥食同源的養生概念深植於東方人的民心，從中草藥尋找有潛力的藥物，是發展生物科技產業的重要的方向。本研究的主要目地乃探討蛹蟲草水抽物是否有降血糖作用，並運用 Streptozotocin (STZ)誘發的第一型糖尿病鼠為病態模式，探討蛹虫草水抽物在糖尿病治療的功效。 在正常大鼠口服蛹蟲草水抽物（10 mg/kg）中發現，血糖值可下降11 %，服藥前後血糖下降有顯著差異 (p＜0.05)，同時胰島素的分泌有明顯的增加。檢測四種胰島素訊號蛋白：Insulin receptor substrate-1 (IRS-1)、glucose transporter-4 (Glut-4)、phosphatidylinositol 3-kinase (PI3-K)、Active human protein kinases （Akt-1），發現訊號有顯著增加的為IRS-1（**P＜0.01）、Glut-4（*P＜0.05）。比較正常與STZ糖尿病動物，分別在口服蛹蟲草水抽物後有顯著的降血糖作用，且糖尿病鼠的降糖百分比顯著優於正常大鼠（* p<0.05）。但對於正常大鼠在葡萄糖耐量試驗(IVGTT)下，並不會因為有口服蛹蟲草水抽物而有改善。進一步，運用STZ糖尿病鼠在胰島素挑戰試驗(ICT)下比較糖尿病鼠口服蟲草與生理食鹽水之各時間點的血糖差異，兩組在60分鐘時有顯著差異（* p<0.05）。 綜合上述，我們發現適量的蛹蟲草水抽物具有降血糖功效，在正常大鼠乃透過胰島素的釋放，經由 IRS-1、與 Glut-4的訊息活化而調降血糖，而在STZ糖尿病鼠具有非胰島素依賴之降糖作用亦能加強胰島素的降糖活性。因此，我們建議蛹蟲草水抽物有運用於糖尿病治療的潛力，特別是作為第一型糖尿病胰島素治療的輔助療法。 致 謝 I 中文摘要 II ABSTRACT III 目錄 IV 表次 I 圖次 II 緒論 1 第一章、文獻探討 4 第一節：糖尿病成因 4 第二節：糖尿病分類 4 第三節: 糖尿病的併發症 5 第四節: 蟲草命名與來源 7 第五節：蛹蟲草培育過程 10 第六節：蛹蟲草機能性成分 17 第七節：蛹蟲草之藥理活性 21 第八節：研究構想 22 第九節：實驗設計 25 第二章：研究材料方法 26 第一節：藥材萃取過程 26 第二節：檢測蟲草素 26 第三節：動物模型 28 第四節：實驗動物步驟 29 第五節：採血及血糖檢測的方法 31 第六節：血清胰島素的測定(ASSAY OF PLASMA INSULIN) 31 第七節：胰島素敏感度之評估（HOMA值） 31 第八節：蛋白質西方轉漬法分析（PROTEIN WESTERN BLOTTING ASSAY） 32 第九節：葡萄糖耐受試驗 INTRAVENOUS GLUCOSE TOLERANCE TEST (IVGTT) 32 第十節：胰島素挑戰試驗 INSULIN CHALLENGE TEST (ICT) 33 第十一節：統計分析(STATISTICAL ANALYSIS) 33 第三章、研究結果 34 第一節：口服蛹蟲草水抽物後，對正常大白鼠降血糖之影響 34 第二節：蛹蟲草水抽物對正常大鼠血中胰島素濃度之影響 36 第三節：蛹蟲草水抽物對正常大鼠胰島素阻抗之影響 38 第四節：蛹蟲草水抽物對大鼠體內胰島素訊號之影響 40 第五節：正常與糖尿病型動物口服蛹蟲草水抽物之血糖差異 42 第六節：蛹蟲草水抽物對於正常大鼠在葡萄糖耐量試驗下血糖之影響 44 第七節：在胰島素挑戰試驗下蛹蟲草水抽物對糖尿病動物血糖之影響 46 第四章、討論 48 第五章、結論 51 參考文獻 52 Balon, T. 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