Publications (16)45.79 Total impact
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Article: Chronic aspirin via dose-dependent and selective inhibition of cardiac proteasome possibly contributed a potential risk to the ischemic heart.
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ABSTRACT: Impaired cardiac proteasome has been reported in ischemic heart and heart failure. Recent data highlighted aspirin as an inhibitor of the ubiquitin-proteasome system, however, it's unclear whether it affects cardiac proteasome functions. Myocardial infarction (MI), sham or normal male SD rats were injected intraperitoneally with high (300mg/kg), low (5mg/kg) aspirin or saline (control) once a day for seven weeks. Parallel experiments were performed in the hypoxia/reoxygenated human ventricular myocytes. Dose-related increases in heart and ventricular weight, and impaired cardiac functions, were found more exacerbated in the aspirin-treated MI rat hearts than the saline-treated MI counterparts. The activity of 26S, 20S and 19S declined by about 30%, or the 20S proteaome subunit β5, β2 and β1 decreased by 40%, 20% and 30%, respectively, in the MI rats compared with the non-MI rats (P<0.05). Compared with the saline-treated MI rats, 26S and 20S in high or low dose aspirin-treated MI rats further decreased by 30% and 20%,β5 by 30% and 12%, and β1 by 40% and 30%, respectively, and the lost activity was correlated with the compromised cardiac functions or the decreased cell viability. The dose-related and selective inhibition of 26S and 20S proteasome, or the 20S proteasome subunit β5 and β1 by aspirin was comparable to their protein expressions in the MI rats and in the cultured cells. The impaired cardiac proteasome, enhanced by chronic aspirin treatment, attenuated the removal of oxidative and ubiquitinated proteins, and chronic aspirin treatment via selective and dose-dependent inhibition of cardiac proteasome possibly constituted a potential risk to ischemic heart.Experimental gerontology 04/2013; · 3.34 Impact Factor -
Article: The rs10947803 SNP of KCNK17 Is Associated with Cerebral Hemorrhage but not Ischemic Stroke in a Chinese Population.
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ABSTRACT: BACKGROUND: KCNK17 (potassium channel, subfamily K, member17) was first discovered to associate with the pathogenesis of ischemic stroke in the first genome-wide association study. The rs10947803 SNP in KCNK17 is significantly associated with ischemic stroke in Caucasian populations. The aim of the present study was to investigate the association with strokes in the Chinese population. METHODS AND RESULTS: A total of 1364 stroke patients and 1293 controls were examined using a case-control methodology. The rs10947803 SNP in KCNK17 was genotyped by a TaqMan real-time PCR assay. The rs10947803 SNP (A allele) of KCNK17 was significantly associated with cerebral hemorrhage (for AA+AC versus CC, unadjusted odds ratio [OR] =1.70; 95% confidence interval [CI], 1.08 to 2.69; P=0.020). After adjustment for age and sex, the association remained significant for AA+AC versus CC, OR =1.65; 95% CI, 1.04 to 2.62; P=0.033. In addition, the rs10947803 SNP in KCNK17 was not associated with ischemic stroke (for AA+AC versus CC, unadjusted OR =0.92; 95% CI, 0.81 to 1.05; P=0.212, after age- and sex-adjustment, OR =0.87; 95% CI, 0.72 to 1.05; P=0.143). CONCLUSIONS: The rs10947803 SNP (A allele) in KCNK17 increases the risk of cerebral hemorrhage but not ischemic stroke in the Chinese population.Neuroscience Letters 02/2013; · 2.11 Impact Factor -
Article: The application of the grey disaster model to forecast epidemic peaks of typhoid and paratyphoid Fever in china.
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ABSTRACT: The objectives of this study were to forecast epidemic peaks of typhoid and paratyphoid fever in China using the grey disaster model, to evaluate its feasibility of predicting the epidemic tendency of notifiable diseases. According to epidemiological features, the GM(1,1) model and DGM model were used to build the grey disaster model based on the incidence data of typhoid and paratyphoid fever collected from the China Health Statistical Yearbook. Model fitting accuracy test was used to evaluate the performance of these two models. Then, the next catastrophe date was predicted by the better model. The simulation results showed that DGM model was better than GM(1,1) model in our data set. Using the DGM model, we predicted the next epidemic peak time will occur between 2023 to 2025. The grey disaster model can predict the typhoid and paratyphoid fever epidemic time precisely, which may provide valuable information for disease prevention and control.PLoS ONE 01/2013; 8(4):e60601. · 4.09 Impact Factor -
Article: Prevalence of sexual activity and associated factors in hypertensive males and females in China: A cross-sectional study.
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ABSTRACT: BACKGROUND: Hypertension is an important factor contributing to sexual dysfunction. The number of people with hypertension is increasing in China, but research into sexual life, which has implications for quality of life, is limited. We aimed to compare sexual activity and the influence of daily behaviors and sexual domain of hypertensive males and females in south China. METHODS: A cross-sectional study was conducted at the health care center of a university-affiliated hospital from 2007 to 2008. We enrolled 502 subjects with hypertension (225 males, 48.79+/-7.39 years old; 277 females, 48.26+/-6.93 years old) and 173 with normotension (82 males, 45.69+/-6.58 years old; 91 females, 46.14+/-7.03 years old), all sexually active. All subjects completed a self-administered questionnaire on sexual activity before a routine physical check-up. Data were collected on sociodemographic and clinical characteristics, use of cigarettes and intake of beverages (including alcohol). RESULTS: Hypertensive and normotensive subjects differed in frequency of orgasms and of sexual satisfaction, as well as duration of sexual activity. For hypertensive men, low frequency of sexual activity, orgasms and satisfaction were associated with unemployed or retired status than physical labor work (odds ratio [OR] 0.28 [95% confidence interval (95% CI) 0.12-0.69], 0.32 [0.12-0.86], 0.33 [0.19-0.88], respectively; p<0.05), and long sexual duration was associated with never drinking alcohol than heavy drinking (OR 4.49 [1.28-6.41]). For hypertensive women, low frequency and duration of sexual activity and low satisfaction were associated with never drinking tea than heavy tea drinking (OR 0.42 [0.18-0.96], 0.49 [0.24-0.98], 0.29 [0.14-0.64], respectively; p<0.05). Medication use and electrocardiography results were not associated with sexual activity for hypertensive patients. CONCLUSIONS: For hypertensive people in China, lifestyle factors are associated with sexual dysfunction, which differs by the sex of the person. Further research needs to examine serum hormone levels to validate the result.BMC Public Health 05/2012; 12(1):364. · 2.00 Impact Factor -
Article: To the editor--correction of transitional zone index.
Heart rhythm: the official journal of the Heart Rhythm Society 12/2011; 8(12):e13-5; author reply e15. · 4.56 Impact Factor -
Article: Effects of urotensin II on functional activity of late endothelial progenitor cells.
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ABSTRACT: Urotensin II (UII) is a potent vasoactive cyclic peptide which has multiple effects on the cardiovascular system. However, the effects of UII on late endothelial progenitor cells (EPCs) are still unclear. The aim of the present study is to investigate whether UII influences the functional activity of late EPCs. Late EPCs were isolated from human umbilical cord blood by Ficoll density gradient centrifugation and treated with UII (10(-10), 10(-9), 10(-8), 10(-7) and 10(-6)M), or vehicle control. Expression of urotensin II receptor (UT) in late EPCs was confirmed by indirect immunofluorescence staining. Late EPCs proliferation, migration and in vitro vasculogenesis activity were assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, transwell chamber assay, and matrigel tube formation assay. Late EPCs adhesive assay was performed by replating cells on fibronectin-coated dishes, and then adherent cells were counted. Incubation with UII increased the migratory, adhesive and in vitro vasculogenesis capacity and inhibited the proliferative activity of late EPCs. Furthermore, these UII-mediated effects on late EPCs were attenuated by pretreatment with the UT antagonist urantide. These findings indicate that UII may exert multiple effects on functional activity of late EPCs through UT.Peptides 11/2011; 33(1):87-91. · 2.43 Impact Factor -
Article: Nicotine improves the functional activity of late endothelial progenitor cells via nicotinic acetylcholine receptors.
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ABSTRACT: The aim of this study is to investigate whether nicotinic acetylcholine receptors (nAChRs) are involved in the modulation of functional activity of late endothelial progenitor cells (EPCs) induced by nicotine. Total mononuclear cells (MNCs) were isolated from human umbilical cord blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture plates. Late EPCs were positive for 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein (DiI-acLDL) uptake and fluorescein-isothiocyanate-conjugated Ulex europaeus agglutinin lectin (UEA-1) binding. Expression of von Willbrand factor (vWF), kinase insert domain receptor (KDR), and α7 nAChR was detected by indirect immunofluorescence staining. Late EPCs of 3-5 passages were treated for 32 h with either vehicle or nicotine with or without pre-incubation of nAChR antagonism, mecamylamine, or α-bungarotoxin. The viability, migration, and in vitro vasculogenesis activity of late EPCs were assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, modified Boyden chamber assay, and in vitro angiogenesis assay, respectively. Late EPCs adhesion assay was performed by replating cells on fibronectin-coated plates, and then adherent cells were counted. Incubation with 10 nmol/L nicotine enhanced viable, migratory, adhesive, and in vitro vasculogenesis capacity of late EPCs. The effect of nicotine on late EPCs can be attenuated by mecamylamine or α-bungarotoxin. In conclusion, nicotine improves the functional activity of late EPCs via nAChRs.Biochemistry and Cell Biology 08/2011; 89(4):405-10. · 2.67 Impact Factor -
Article: Green tea may be benefit to the therapy of atrial fibrillation.
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ABSTRACT: Atrial fibrillation (AF) is the most common cardiac arrhythmia in clinical practice. Systemic inflammatory state, oxidative stress injury, and atrial fibrosis are identified as the main mechanisms for AF. Considering the multifactorial mechanisms of AF, a novel therapeutic agent with multi-bioactivities should be presented. Regular consumption of green tea has been associated with a reduced risk of coronary heart disease and against a large number of pathologic conditions. Recent results indicate that green tea extract, especially (-)-epigallocatechin-3-gallate, could effectively decrease inflammatory factors secretion, antagonize oxidation, and inhibit matrix metalloproteinase activities. Inhibition of inflammation, modulation of oxidative stress, and targeting tissue fibrosis represent new approaches in tackling AF; therefore, green tea may be an innovative therapeutic candidate to prevent the occurrence, maintenance, and recurrence of AF.Journal of Cellular Biochemistry 03/2011; 112(7):1709-12. · 2.87 Impact Factor -
Article: Effects of osteopontin on functional activity of late endothelial progenitor cells.
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ABSTRACT: The aim of this study is to investigate the effect of osteopontin (OPN) on functional activity of late endothelial progenitor cells (EPCs). Total mononuclear cells (MNCs) were isolated from human umbilical cord blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture plates. Late EPCs were positive for both 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein (DiI-acLDL) and fluorescein-isothiocyanate-conjugated Ulex europaeus agglutinin lectin (UEA-1). Expression of von Willbrand factor (vWF) and kinase insert domain receptor (KDR) were detected by indirect immunofluorescence staining. Late EPCs of 3-5 passages were treated for 24 h with OPN (to make a series of final concentration: 0.005 µg/ml, 0.01 µg/ml, 0.05 µg/ml, 0.5 µg/ml, 2.5 µg/ml), or vehicle control. The proliferation, migration, and in vitro vasculogenesis activity of late EPCs were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, modified Boyden chamber assay and an in vitro angiogenesis assay, respectively. Late EPCs adhesion assay was performed by replating cells on fibronectin-coated plates, and then adherent cells were counted. Incubation with OPN dose-dependently inhibited the proliferative, adhesive, and in vitro vasculogenesis capacity and increased migratory activity of late EPCs.Journal of Cellular Biochemistry 02/2011; 112(7):1730-6. · 2.87 Impact Factor -
Article: Cardiovascular effects of sexual activity.
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ABSTRACT: Sexuality is a major way of intimacy in human being and it is very important in gender relationship, contributing to over all health. However, since association between sexual activity and sudden death determined by forensic autopsies related to cardiac or cerebral causes has been reported, some people with heart disease often abstain from sexual activity that could affect the quality of life. It is therefore important to learn the physical demand of sexual activity and the risk it may trigger. For decades, the cardiologists have conducted observational studies and clinical trials on healthy volunteers or patients. The most common indices responding to cardiovascular risks of sexuality were variances of blood pressure (BP) and heart rate (HR), monitored by ambulatory blood pressure and dynamic electrocardiogram recording. BP and HR increase during the coitus just briefly and quickly recover to baseline level. Peak coital BP occurred at onset of plateau phase and quickly decreased, instead of emerged at orgasm as most people supposed. The metabolic equivalent of energy expenditure during the orgasm was relatively modest when compared with other physical exertion such as cycling. Epidemiological studies have suggested that sexual activity has favorable effect on health in the long term. This review summarizes and discusses the advances in the researches dealing with cardiovascular effects of sexual activity to better inform the cardiac patients.The Indian journal of medical research 12/2009; 130(6):681-8. · 1.84 Impact Factor -
Article: The 1425G/A SNP in PRKCH is associated with ischemic stroke and cerebral hemorrhage in a Chinese population.
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ABSTRACT: PRKCH (the gene encoding protein kinase C eta) has a role in the pathogenesis of ischemic stroke. The 1425G/A SNP in PRKCH (rs2230500) is significantly associated with ischemic stroke in Japanese. The aim of the present study is to investigate the associations in ischemic stroke and other types of stroke in the Chinese population. A total of 1209 patients with stroke and 1174 controls were examined using a case-control methodology. The 1425G/A SNP in PRKCH was genotyped by allele-specific real-time PCR assay. The 1425G/A SNP in PRKCH was significantly associated with both ischemic stroke (odds ratio [OR]=1.31; 95% confidence interval [CI], 1.08 to 1.60; P=0.0058) and cerebral hemorrhage (OR=1.94; 95% CI, 1.21 to 3.10; P=0.0054) under a dominant model. Even after age- and sex-adjustment, the significant associations remained (in ischemic stroke, for AA+AG versus GG, OR=1.37, 95% CI, 1.12 to 1.67, P=0.0019; in cerebral hemorrhage, for AA+AG versus GG, OR=1.96, 95% CI, 1.21 to 3.19, P=0.0064). The 1425G/A SNP in PRKCH increases the risk of both ischemic stroke and cerebral hemorrhage in the Chinese population.Stroke 07/2009; 40(9):2973-6. · 5.73 Impact Factor -
Article: The functional variant rs1048990 in PSMA6 is associated with susceptibility to myocardial infarction in a Chinese population.
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ABSTRACT: A recent case-control study reported that a functional single nucleotide polymorphism (SNP) in the proteasome subunit alpha type 6 gene (PSMA6) (rs1048990, C/G) was associated with susceptibility to myocardial infarction (MI) in the Japanese population. Replication studies have been performed in European and other Japanese study samples, but the results were not conclusive. The purpose of the present study was to determine whether this locus confers significant susceptibility to MI in a Chinese population. We conducted a case-control association study on a cohort of 1884 MI patients and 2643 unrelated controls from the Chinese population. Genotyping of the rs1048990 SNP was performed by the Allele-specific Real Time PCR method. We found that rs1048990 was significantly associated with MI (adjusted for age and sex, odds ratio 1.22, p=0.000005, allele frequency model; odds ratio 1.44, p=0.0000025; recessive model; odds ratio 1.56, p=0.00000048, additive model). A meta-analysis yielded a combined OR for MI of 1.15 (95% CI: 1.11-1.21) with an allele frequency model, 1.37 (95% CI: 1.23-1.51) with a recessive model and 1.44 (95% CI: 1.29-1.60) with an additive model. There was no relationship between rs1048990 and age, sex or other conventional cardiovascular risk factors. Our results indicate that the PSMA6 variant rs1048990 is a risk factor of myocardial infarction in the Chinese population.Atherosclerosis 03/2009; 206(1):199-203. · 3.79 Impact Factor -
Article: The effect of delayed preconditioning on connexin 43 in ischemic myocardium.
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ABSTRACT: The objective of this study is to investigate the effects of preconditioning on the restoration and distribution of connexin 43 (Cx43) in ischemic myocardium in dogs. In this study, 40 dogs were randomly divided into 5 groups of 8 as follows: control, 0hI-R (ischemia followed by 0 h reperfusion), 6hI-R (ischemia followed by 6 h reperfusion), 24hI-R (ischemia followed by 24 h reperfusion), and 48hI-R (ischemia followed by 48 h reperfusion). Four dogs in each group were preconditioned with brief episodes of ischemia prior to the respective treatments and were referred as the PC groups, while the other 4 were not preconditioned and were referred as the nonPC groups. The myocardial ischemia was induced by ligation of the left anterior descending coronary artery. The expression and distribution of Cx43 within the ischemic myocardium were measured by Western blot analysis and studied using laser confocal microscopy using a double-label immunohistochemistry technique. Compared with the control group, there was a significant reduction in Cx43 content within ischemic myocardium of all test groups both with and without PC (P < 0.01, P < 0.05). Within the 0hI-R, 6hI-R, and 24hI-R groups, an insignificant difference was found in the expression and distribution of Cx43 within the ischemic region between the PC and the nonPC groups. However, in the 48hI-R group, the area and intensity of Cx43 staining within the ischemic region of the PC dogs were significantly larger and more intense than those of the nonPC dogs (P < 0.01), and the ratio of Cx43 pixel density in intercalated disk areas to that in side-to-side junction areas in the PC dogs was significantly greater than that in nonPC dogs (P < 0.01). Our results suggest that preconditioning has a significant effect on the restoration and distribution of Cx43 in the ischemic myocardium in dogs after 48 h. Hence, preconditioning may be a plausible cause for the observed reductions in cardiac arrhythmias.Biochemistry and Cell Biology 04/2007; 85(2):175-81. · 2.67 Impact Factor -
Article: Physiological testosterone stimulates tissue plasminogen activator and tissue factor pathway inhibitor and inhibits plasminogen activator inhibitor type 1 release in endothelial cells.
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ABSTRACT: There is a striking gender difference in atherosclerotic vascular disease. For decades, testosterone was considered detrimental to the cardiovascular system. Recent studies, however, have presented some alternative results. The aim of this study was to evaluate the effect of testosterone, using physiological and supraphysiological concentrations, on antigen and mRNA levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1), and tissue factor pathway inhibitor (TFPI) released by human umbilical vein endothelial cells and to investigate the cellular mechanism. Cells within 2-3 passages were cultured in 25 cm(2) flasks or plated onto 96-well plates with a density of about 1 x 10(5) cells/mL as recommended. The cells were incubated in the presence or absence of testosterone (3, 30, 3 x 10(3), 3 x 10(4) nmol/L) for 48 h. Levels of tPA, PAI-1, and TFPI antigen were assayed with ELISA kits. Reverse transcriptase PCR was carried out to detect tPA, PAI-1, and TFPI mRNA levels. Cells were incubated in androgen-receptor antagonist (flutamide 10 micromol/L) or aromatase inhibitor (aminoglutethimide 50 micromol/L) for 3 h, and then the experiments were repeated. Testosterone at a physiologic concentration (30 nmol/L) increased the antigen levels of tPA and TFPI significantly (P < 0.05). However, tPA and TFPI levels were markedly reduced (P < 0.05) at a larger dose (3 x 10(4) nmol/L). On the other hand, PAI-1 antigen levels decreased significantly at the testosterone concentrations ranging from 3 to 3 x 10(4) nmol/L (P < 0.05). The change in the levels of tPA and TFPI were reflected in the corresponding change in mRNA levels. Flutamide attenuated the effect of testosterone at physiological concentration (30 nmol/L). The results demonstrated that testosterone at physiological concentrations may have a beneficial influence on the haemostatic system through enhancement of anticoagulant activity, resulting from stimulation of TFPI and tPA expression and inhibition of PAI-1 secretion by the endothelium.Biochemistry and Cell Biology 04/2007; 85(2):246-51. · 2.67 Impact Factor -
Article: Inhibition of the ubiquitin-proteasome system: a new avenue for atherosclerosis.
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ABSTRACT: The ubiquitin-proteasome system (UPS) is thought to be functionally active in atherosclerosis (AS) lesions. Aspirin was found to be a potent inhibitor of the UPS in some tumour studies; however, its effect on AS remains to be demonstrated in vivo. New Zealand rabbits were placed on a normal diet (N) or on a normal diet with aspirin (NI) or on an atherogenic diet without (H) or with aspirin (HI) for 12 weeks. Proteasome activity, concentrations of plasma lipids and levels of peroxidation were determined. Ubiquitin/ubiquitin-conjugates (Ub), IkappaBalpha, phosphorylated IkappaB (pIkappaBalpha) and p65 were investigated by Western blotting or immunochemistry. Concentrations of plasma lipids and peroxidation levels were higher in H or HI vs. N or NI. Histological analysis showed that atheroma was increased in H. Ub and IkappaBalpha were mainly localised in subendothelium and media vascular smooth muscle cells. Western blots revealed that Ub, IkappaBalpha, and pIkappaBalpha were increased, whereas p65 was lower in HI vs. H. The activity of the 20S proteasome was functionally active in H vs. N, NI or HI, while the 26S proteasome was not affected in any of the groups. Aspirin can attenuate the pathogenesis of atheroma formation, the degradation of IkappaBalpha and pIkappaBalpha, and lower the expression of p65, indicating that its therapeutic effects on AS may be via inhibition of the UPS.Clinical Chemistry and Laboratory Medicine 02/2006; 44(10):1218-25. · 2.15 Impact Factor -
Article: [Study on N-nitroso compound in food and its relevant risk factors for esophageal cancer].
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ABSTRACT: To study multiple risk factors of N-nitroso compounds (NOC) in high- and low-risk areas for esophageal cancer in southern China. The samples of 24-hr diets and 12-hr overnight urine were collected from 120 male healthy subjects (35-64 years old) selected by a 3-stage random cluster sample procedure in each of the high-risk area (Nanao County) and low-risk area (Lufeng County) for esophageal cancer. The urinary samples were respectively collected from undosed subjects, subjects ingested 500 mg L-proline (together with 200 mg ascorbic acid ) and subjects ingested 500 mg proline. The levels of total NOC (TNOC), N-nitrosamino acids (NAAs), volatile N-nitroso compounds and reductive ascorbic acid (VC) in the samples were measured. By unconditional logistic stepwise regression model, we analyzed the association between the multiple factors of NOC and esophageal cancer mortality. The factors included the intake and excretion levels of various kinds of NOC, the ability of NAAs endogenous formation and its inhibition by VC, and nutrition status of VC in the body. The results of unconditional logistic stepwise regression showed that risk factors entered the model were diet TNOC content (OR 9.613, 95% CI 1.921-48.115) and urinary NAAs level after ingested VC (OR 1.137, 95 % CI 1.001-1.298). The higher level of diet TNOC and the lower inhibition ability of NOC endogenous formation by VC were important risk factors on NOC etiology of esophageal cancer in southern China.Wei sheng yan jiu = Journal of hygiene research 06/2005; 34(3):350-2.
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2011–2013
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Shantou University
- Department of Cardiology
Shantou, Guangdong Sheng, China
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