[Show abstract][Hide abstract] ABSTRACT: Background
As an important traditional Tibetan (veterinary) medicine, the flowers of Meconopsis punicea (family Papaveraceae) have been used to treat pain, fever, cough, inflammation, liver heat and lung heat of humans and animals by local people for thousands of years. In this paper, we aimed to investigate the antinociceptive and anti-tussive activities of the ethanol extract of M. punicea (EEM).
Firstly, HPLC was used to analyze the main constituents of the ethanol extract of M. punicea. In animal experiments, the acetic acid-induced writhing response test, hot plate test, barbiturate-induced sleeping time and formalin tests were used to evaluate the antinociceptive activity. Then, ammonia-induced coughing and sulfur dioxide-induced coughing tests in mice as well as the phenol red secretion in trachea test were used to investigate the anti-tussive activity of the extract. Finally, an acute toxicity study was carried out.
The results showed that alkaloids and flavonoids were the main compounds in the ethanol extract of M. punicea flowers. The extract at 125, 250 and 500 mg/kg had good antinociceptive and anti-tussive activities in mice with a dose-dependent manner.
These findings suggested that EEM has significant bioactivities, and the active components of M. punicea should be studied further.
BMC Complementary and Alternative Medicine 05/2015; 15(1). DOI:10.1186/s12906-015-0671-y · 1.88 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Usnic acid, a major active compound in lichens, was first isolated in 1884. Since then, usnic acid and its sodium salt (sodium usnic acid) have been used in medicine, perfumery, cosmetics, and other industries due to its extensive biological activities. However, its acaricidal activity has not been studied. In this paper, we investigated the acaricidal activity of usnic acid and sodium usnic acid against Psoroptes cuniculi in vitro. After evaluating the acaricidal activity and toxicity of usnic acid and sodium usnic acid in vitro, the results showed that at doses of 250, 125, and 62.5 mg/ml, usnic acid and sodium usnic acid can kill mites with 91.67, 85.00, and 55.00 % and 100, 100, and 60.00 % mortality after treatment 24 h. The LT50 values were 4.208, 8.249, and 16.950 h and 3.712, 7.339, and 15.773 h for usnic acid and sodium usnic acid, respectively. Sodium usnic acid has a higher acaricidal activity than usnic acid, which may be related to the difference in their structures.
Parasitology Research 04/2014; 113(6). DOI:10.1007/s00436-014-3910-0 · 2.33 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Psoroptes cuniculi can parasitise the ear canal of the rabbit, and cause the afflicted animals to cease feeding and become severely debilitated, sometimes resulting in death. In this study, we examined the oxidative status and inflammatory level of the peripheral blood of rabbits infested with Psoroptes cuniculi and investigated the pathogenesis of this disease.
A total of 24 rabbits were divided into a healthy rabbit group and two infested rabbit groups. After weighing the rabbits, approximately 5 ml of blood was obtained from each animal. Then, the blood serum was extracted and used to assess the levels of antioxidant enzymes and inflammatory factors.
Compared to the healthy rabbits, the activities of catalase and glutathione-S-transferase and the level of malonyldialdehyde were increased, but the activity of superoxide dismutase was reduced in the infested rabbits. At the same time, a variety of inflammatory cells were activated, and the levels of inflammatory factors such as prostaglandin E2, interleukin-6, interleukin-8 and transforming growth factor-beta1 were increased in peripheral blood.
Animal acariasis was associated with immunosuppressive disorders and inflammatory reaction. These results advance our understanding of the pathogenesis of Psoroptes cuniculi infestation in rabbits and can help guide the effectual treatment of this disease in clinics.
[Show abstract][Hide abstract] ABSTRACT: In this study we aimed to survey and investigate the medicinal plants which are used to treat the veterinary diseases in Ruoergai region, Sichuan province, China. Meanwhile, the important medicinal plants were collected and identified for the further study.
Twenty folk veterinary practitioners from 8 township animal husbandry and veterinary stations in Ruoergai region were investigated and interviewed. The important local medicinal materials, including plants, animals and mineral drugs, were collected by scientific methods and identified by the pharmacognosist of Lanzhou University, China.
According to the investigation, only 20 folk veterinary practitioners still used 129 species of traditional medicine to treat the livestock`s diseases. In these medicine, 93 species were native and Ranunculaceae (12, 12.90%), Compositae (11, 11.83%), Papaveraceae (7, 7.53%) were the predominant families. At the same time, herbs (36.56%) are the most widely used part of plant, and respiratory diseases (21.02%) and gastrointestinal diseases (19.89%) were the main animal's diseases in this region.
Ethno-veterinary medicine made an extraordinary contribution to the sound development of animal husbandry in Ruoergai. But the inherit, protect and development should be paid more attentions in the future, and the species which have not been studied should be developed priority to find biological activities and new bioactive compounds further.
Journal of ethnopharmacology 05/2012; 142(2):390-400. DOI:10.1016/j.jep.2012.05.006 · 2.94 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Lonicera japonica Thunb. (Caprifoliaceae), a widely used traditional Chinese medicine, was known as Jin Yin Hua (Chinese: ), Ren Dong and Japanese honeysuckle. It was taken to treat the exopathogenic wind-heat, epidemic febrile diseases, sores, carbuncles and some infectious diseases. At the same time, Lonicera japonica could be used as healthy food, cosmetics, ornamental groundcover, and so on.
The present paper reviewed the ethnopharmacology, the biological activities, toxicology and phytochemistry of Lonicera japonica.
Information on Lonicera japonica was gathered via the Internet (using Google Scholar, Baidu Scholar, Elsevier, ACS, Medline Plus, CNKI and Web of Science) and libraries. Additionally, information also was obtained from some local books and brilliant scholars on ethnopharmacology.
More than 140 chemical compounds have been isolated, and the main compositions are essential oils, organic acids and flavones, etc. Lonicera japonica and its active principles possess wide pharmacological actions, such as anti-inflammatory, antibacterial, antiviral, antioxidative and hepatoprotective activities.
As an important traditional Chinese medicine, further studies on Lonicera japonica can lead to the development of new drugs and therapeutics for various diseases, and how to utilize it better should be paid more attentions.
Journal of ethnopharmacology 08/2011; 138(1):1-21. DOI:10.1016/j.jep.2011.08.016 · 2.94 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Phlomis umbrosa Turcz has been used as the traditional medicine for thousands of years in China. In this paper, the acetic acid-induced writhing test, the hot plate test, the carrageenan-induced paw edema test, the xylene-induced ear swelling test, and the acetic acid-induced Evans blue leakage and leukocyte infiltration test were used to investigate the antinociceptive and anti-inflammatory activities of the aqueous extract of this plant (25, 50 and 100mg/kg i.p.). Good dose-dependent effects were obtained in most of these tests, except in the hot plate test and the acetic acid-induced Evans blue leakage test. TLC and HPLC analyses showed iridoid glucosides were the main compositions of this extract. These findings suggested that the aqueous extract of P. umbrosa has significant antinociceptive and anti-inflammatory activities.