Yanfang Ju

Publications (3)9.46 Total impact

• Article: Mutation of the Nrf2 gene in non-small cell lung cancer.

  Yi Hu, Yanfang Ju, Dongmei Lin, Zhikuan Wang, Yurong Huang, Sujie Zhang, Chao Wu, Shunchang Jiao
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  ABSTRACT: Nrf2 (NFE2L2) is a transcription factor belonging to the Cap'N'Collar subfamily of basic-leucine zipper (bZIP) family of transcription factors, which plays a significant role in adaptive responses to oxidative stress. To investigate the relationship of between the mutation of Nrf2 gene and non-small cell lung cancer (NSCLC), in this study, we sequenced the Nrf2 gene from a total of 103 patients with NSCLC and corresponding blood samples. It is found that there is a discordance of Nrf2 mutations between NSCLC and corresponding blood samples. These differences may indicate that the variants in the Nrf2 gene are associated with an increased risk for lung cancer. In addition, the factor of smoking status is observed to play important roles in triggering the occurrence of the disorder.
  Molecular Biology Reports 10/2011; 39(4):4743-7. · 2.93 Impact Factor
• Article: Antigenically dominant proteins within the human liver mitochondrial proteome identified by monoclonal antibodies.

  YanFang Ju, JinJu Yang, Rong Liu, XiaoLan Liu, XueMei Du, Li Liu, ZhiCheng Chen, Jun Chi, ShuEr Liu, Yuan Gao, JianEn Gao, ShunChang Jiao, FuChu He, QiHong Sun
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  ABSTRACT: Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and calcium signaling. It could also increase our understanding about the mechanisms that promote mitochondrial disease. To identify proteins that are antigenically dominant in human liver mitochondria, we generated >240 hybridoma cell lines from native mitochondrial proteins after cell fusion, screening, and cloning. Antibodies that recognized mitochondrial proteins were identified by screening human liver cDNA expression libraries. In this study, we identified 6 major antigens that were recognized by at least 2 different monoclonal antibodies (mAbs). The proteins that were antigenically dominant were: acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase), aldehyde dehydrogenase 1 family member A1, carbamoyl phosphate synthetase 1, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex), enoyl coenzyme A hydratase 1, and hydroxysteroid (11-beta) dehydrogenase 1. We also determined the subcellular localizations of these enzymes within the mitochondria using immunohistocytochemistry. We believe that these well-characterized antibodies will provide a valuable resource for the Human Liver Proteome Project (HLPP), and will make studies aimed at investigating liver mitochondrial function far easier to perform in future. Our results provide strong evidence that, (i) depletion of dominant proteins from liver mitochondrial samples is possible and, (ii) the approaches adopted in this study can be used to explore or validate protein-protein interactions in this important organelle.
  Science China. Life sciences 01/2011; 54(1):16-24. · 2.02 Impact Factor
• Article: Proteomics-based generation and characterization of monoclonal antibodies against human liver mitochondrial proteins.

  Jianen Gao, Yuan Gao, Yanfang Ju, Jinju Yang, Qiong Wu, Jinchao Zhang, Xuemei Du, Zhaoqing Wang, Yingbo Song, Hui Li, [......], Han Xu, Xiaolan Liu, Jinlan Wang, Yangjun Zhang, Yun Cai, Yufang Cui, Xiaohong Qian, Fuchu He, Ming Li, Qi-Hong Sun
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  ABSTRACT: Monoclonal antibodies (mAbs) have the potential to be a very powerful tool in proteomics research to determine protein expression, quantification, localization and modification, as well as protein-protein interactions, especially when combined with microarray technology. Thus, a large amount of well-characterized and highly qualified antibodies are needed in proteomics. Purified antigen, which is not always available, has proven to be one of the rate-limiting steps in mAb large-scale generation. Here we describe our strategies to establish a murine hybridoma cell bank for human liver mitochondria using unknown native proteins as the immunogens. The antibody-recognized mitochondrial proteins were identified by MS following immunoprecipitation (IP), and by screening of human liver cDNA expression library. We found that the established antibodies reacted specifically with a number of important enzymes in mitochondria. The subcellular localization of these antigens in mitochondria was further confirmed by immunohistocytochemistry. A panel of antibodies was also tested for their ability to capture and deplete the targeting proteins and complexes from the total mitochondrial proteins. We believe these well-characterized antibodies would be useful in various applications for Human Liver Proteome Project (HLPP) when the scale of this hybridoma cell bank is enlarged significantly in the near future.
  PROTEOMICS 02/2006; 6(2):427-37. · 4.51 Impact Factor