Wibool Piyawattanametha

King Mongkut's Institute of Technology Ladkrabang, Krung Thep, Bangkok, Thailand

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Publications (74)108.67 Total impact

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    ABSTRACT: Protein p(16INK4a) (p16) is a well-known biomarker for diagnosis of human papillomavirus (HPV) related cancers. In this work, we identify novel p16 binding peptides by using phage display selection method. A random heptamer phage display library was screened on purified recombinant p16 protein-coated plates to elute only the bound phages from p16 surfaces. Binding affinity of the bound phages was compared with each other by enzyme-linked immunosorbent assay (ELISA), fluorescence imaging technique, and bioinformatic computations. Binding specificity and binding selectivity of the best candidate phage-displayed p16 binding peptide were evaluated by peptide blocking experiment in competition with p16 monoclonal antibody and fluorescence imaging technique, respectively. Five candidate phage-displayed peptides were isolated from the phage display selection method. All candidate p16 binding phages show better binding affinity than wild-type phage in ELISA test, but only three of them can discriminate p16-overexpressing cancer cell, CaSki, from normal uterine fibroblast cell, HUF, with relative fluorescence intensities from 2.6 to 4.2-fold greater than those of wild-type phage. Bioinformatic results indicate that peptide 'Ser-His-Ser-Leu-Leu-Ser-Ser' binds to p16 molecule with the best binding score and does not interfere with the common protein functions of p16. Peptide blocking experiment shows that the phage-displayed peptide 'Ser-His-Ser-Leu-Leu-Ser-Ser' can conceal p16 from monoclonal antibody interaction. This phage clone also selectively interacts with the p16 positive cell lines, and thus, it can be applied for p16-overexpressing cell detection. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd.
    Journal of Peptide Science 03/2015; DOI:10.1002/psc.2726 · 1.86 Impact Factor
  • Zhen Qiu, Wibool Piyawattanametha
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    ABSTRACT: Fiber-optical microendoscopy has recently been an essential medical diagnostic tool for patients in investigating tissues in vivo due to affordable cost, high quality imaging performance, compact size, high-speed imaging, and flexible movement. Microelectromechanical systems (MEMS) scanner technology has been playing a key role in shaping the miniaturization and enabling high-speed imaging of fiber-optical microendoscopy for over 20 years. In this article, both review of MEMS based fiber-optical microendoscopy for optical coherence tomography, confocal, and two-photon imaging will be discussed. These advanced optical endoscopic imaging modalities provide cellular and molecular features with deep tissue penetration enabling guided resections and early cancer assessment.
    Displays 01/2015; DOI:10.1016/j.displa.2014.12.001 · 1.21 Impact Factor
  • Zhen Qiu, Wibool Piyawattanametha
    IEEE Journal of Selected Topics in Quantum Electronics 01/2015; DOI:10.1109/JSTQE.2015.2389530 · 3.47 Impact Factor
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    ABSTRACT: Increasing levels of antibiotic resistance by Staphyloccocus aureus have posed a need to search for non-antibiotic alternatives. This study aimed to assess the inhibitory effects of crude and fractionated cell-free supernatants (CFS) of locally isolated lactic acid bacteria (LAB) against a clinical strain of S. aureus. A total of 42 LAB strains were isolated and identified from fresh vegetables, fresh fruits and fermented products prior to evaluation of inhibitory activities. CFS of LAB strains exhibiting a stronger inhibitive effect against S. aureus were fractionated into crude protein, polysaccharide and lipid fractions. Crude protein fractions showed greater inhibition against S. aureus compared to polysaccharide and lipid fractions, with a more prevalent effect from Lactobacillus plantarum 8513 and L. plantarum BT8513. Crude protein, polysaccharide and lipid fractions were also characterised with glycine, mannose and oleic acid being detected as the major component of each fraction, respectively. Scanning electron microscopy revealed roughed and wrinkled membrane morphology of S. aureus upon treatment with crude protein fractions of LAB, suggesting an inhibitory effect via the destruction of cellular membrane. This research illustrated the potential application of fractionated extracts from LAB to inhibit S. aureus for use in the food and health industry.
    Beneficial Microbes 09/2014; DOI:10.3920/BM2014.0021 · 1.50 Impact Factor
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    Annals of Microbiology 01/2014; DOI:10.1007/s13213-014-0949-1 · 1.04 Impact Factor
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    ABSTRACT: Our work demonstrates a MEMS based handheld dual-axis confocal microscope for cervical cancer screening. Imaging demonstration is performed with plant and animal tissue biopsies. The data is collected and displayed in real time with 2-5 Hz frame rates.
    SPIE Photonics West; 10/2013
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    ABSTRACT: A new chapter in the history of medical diagnosis happened when the first X-ray technology was invented in the late 1800s. Since then, many non-invasive and minimally invasive imaging techniques have been invented for clinical diagnosis to research in cellular biology, drug discovery, and disease monitoring. These imaging modalities have leveraged the benefits of significant advances in computer, electronics, and information technology and, more recently, targeted molecular imaging. The development of targeted contrast agents such as fluorescent and nanoparticle probes has made it possible to selectively view specific biological events and processes in both in vivo and ex vivo systems with great sensitivity and selectivity. Thus, these contrast agents or targeted molecular probes have become a mainstay in modern medicinal and biological research. Many promising results have showed potentials to translate to clinical applications. In this review, we describe a discussion of employing imaging probes and optical microendoscopic imaging techniques for cancer diagnosis to enable favorable treatment outcome.
    Advanced drug delivery reviews 10/2013; DOI:10.1016/j.addr.2013.09.012 · 11.96 Impact Factor
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    ABSTRACT: A MEMS based handheld multi-spectral confocal microscope has been developed as a noninvasive imaging instrument for cervical cancer screening. Tissue imaging depth of up to 400 μm was demonstrated. The maximum speed of image collection is up to 10 Hz with field of view (FOV) around 200×158 pixel2 size. Our handheld prototype is able to visualize cancer cells with cellular resolution (transverse resolution = 5 μm and axial resolution = 6 μm). Biological imaging demonstration was carried out on cell culture and tissues of human cervix.
    Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on; 01/2013
  • W. Piyawattanametha
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    ABSTRACT: Progress toward early diagnosis of cancer would have important clinical benefits in reducing cancer mortality; thus, there is an important need to image cellular features of cancer in vivo and in real-time. In this paper, we describe a review of microelectromechanical systems (MEMS) scanner based endoscopic optical coherence tomography (OCT), two-photon (2P), and confocal imaging. These modalities can provide submicron- and micro-scale resolution to reveal both cells and molecular features of early cancer diagnosis.
    Optical MEMS and Nanophotonics (OMN), 2013 International Conference on; 01/2013
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    ABSTRACT: In this paper, two different designs of two-dimensional MEMs scanner were tested to find their characteristic responses in dynamic mode by applying a fixed voltage at different electrical biasing waveforms: sinusoidal, triangle, square and saw-tooth waveforms. The collected data from the experiment has proven that the significance of MEMS scanner design and driving input signals.
    IEEE International Conference on Electron Devices and Solid State Circuit (EDSSC); 12/2012
  • Numfon Khemthongcharoen, Athisake Ruangpracha, Wibool Piyawattanametha
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    ABSTRACT: This work demonstrates the application of phage display technology for molecular diagnosis utility. We propose a novel phage-displayed peptide which specifically bind to p16INK4a, a cervical cancer biomarker. Whole phage particles were developed as a molecular tracer for ex vivo cells imaging technique. Increase in specific phages binding to p16INK4a overexpressed cells is improved when the cells were initially permeabilized in order to make phage penetrable pores on the target cell membranes. We also proved that fluorescence signal could be obviously enhanced due to tremendous interaction sites for fluorescence dye labeling available on capsid proteins around phage particles. Evaluation of p16INK4a binding phages to discriminate between p16INK4a overexpressed cervical cancer cells versus normal fibroblast cells demonstrated higher fluorescence intensity of 2.5 fold over native phages.
    IEEE 6th International Conference on Nano/Molecular Medicine and Engineering (NANOMED), 2012; 11/2012
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    ABSTRACT: We present a 9.6-mm fiber-coupled probe for femtosecond laser microsurgery and nonlinear imaging. Towards enabling clinical use, we successfully reduced the volume of our earlier 18-mm surgery probe by 5 times, while improving optical performance.
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    ABSTRACT: Near-infrared confocal microendoscopy is a promising technique for deep in vivo imaging of tissues and can generate high-resolution cross-sectional images at the micron-scale. We demonstrate the use of a dual-axis confocal (DAC) near-infrared fluorescence microendoscope with a 5.5-mm outer diameter for obtaining clinical images of human colorectal mucosa. High-speed two-dimensional en face scanning was achieved through a microelectromechanical systems (MEMS) scanner while a micromotor was used for adjusting the axial focus. In vivo images of human patients are collected at 5 frames/sec with a field of view of 362×212 μm(2) and a maximum imaging depth of 140 μm. During routine endoscopy, indocyanine green (ICG) was topically applied a nonspecific optical contrasting agent to regions of the human colon. The DAC microendoscope was then used to obtain microanatomic images of the mucosa by detecting near-infrared fluorescence from ICG. These results suggest that DAC microendoscopy may have utility for visualizing the anatomical and, perhaps, functional changes associated with colorectal pathology for the early detection of colorectal cancer.
    Journal of Biomedical Optics 02/2012; 17(2):021102. DOI:10.1117/1.JBO.17.2.021102 · 2.75 Impact Factor
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    ABSTRACT: We demonstrated a handheld multispectral fluorescence confocal microscope for cervical cancer diagnostic using dual-axis confocal microscope architecture and a microelectromechanical systems scanner. The real time images are acquired with frame rate up to 15 Hz.
    Optical MEMS and Nanophotonics (OMN), 2012 International Conference on; 01/2012
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    ABSTRACT: Our work demonstrated a MEMS based handheld dual-axis confocal microscope for cervical cancer screening. Imaging demonstration is performed with plants and animal tissue biopsies. The data is collected and displayed in real time with 2-5 Hz.
    Electrical Engineering/Electronics, Computer, Telecommunications and Information Technology (ECTI-CON), 2012 9th International Conference on; 01/2012
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    ABSTRACT: We describe a 2-D MEMS scanner for a handheld multispectral confocal microscope for early detection of cervical cancer. The MEMS scanner has an inner gimbal design with torsional springs separated from the reflectors to reduce light loss while maintaining chip size to 3.25 × 3.25 mm2. The devices are large-scale batch fabricated using a double layer SOI process. The scanner has electrostatic optical deflection angles of 3.25° for the inner axis at 75 V and ±1.6° for the outer axis at 60 V. The device has resonance frequencies of 2.84 kHz and 452 Hz for the inner and outer axis torsional modes respectively.
    Optical MEMS and Nanophotonics (OMN), 2012 International Conference on; 01/2012
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    Wibool Piyawattanametha, Zhen Qiu
    Microelectromechanical Systems and Devices, 12/2011; , ISBN: 978-953-51-0306-6
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    ABSTRACT: The problem of limited field of view when visualizing biomedical specimens with high-magnification microscopes can be solved by using image mosaicing or image stitching technique to merge multiple microscopic images acquired from the specimens to create a seamless stitched image. The image mosaicing technique is commonly used in automatically map construction as a path planning system for vision-based robot navigation. By using this technique as the application of digital pathology, it is helpful for pathologists to view different parts of the specimens at high resolution on computer display. In this paper, we present an automatic image mosaicing algorithm which is implemented by using motion estimation technique in image registration step i.e. estimating the two-dimensional translation between each pair of image sequence by using optical flow and phase correlation methods. The aligned images are then fused together using multi-resolution image blending. These algorithms were accomplished to demonstrate the synthetic image sequences in microscopic resolution.
    IEEE International Conference on Robotics and Biomimetics (ROBIO), 2011; 12/2011
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    ABSTRACT: We present the optical design of a 9.6-mm diameter fiber-coupled probe for combined femtosecond laser microsurgery and nonlinear optical imaging. Towards enabling clinical use, we successfully reduced the dimensions of our earlier 18-mm microsurgery probe by half, while improving optical performance. We use analytical and computational models to optimize the miniaturized lens system for off-axis scanning aberrations. The optimization reveals that the optical system can be aberration-corrected using simple aspheric relay lenses to achieve diffraction-limited imaging resolution over a large field of view. Before moving forward with custom lenses, we have constructed the 9.6-mm probe using off-the-shelf spherical relay lenses and a 0.55 NA aspheric objective lens. In addition to reducing the diameter by nearly 50% and the total volume by 5 times, we also demonstrate improved lateral and axial resolutions of 1.27 µm and 13.5 µm, respectively, compared to 1.64 µm and 16.4 µm in our previous work. Using this probe, we can successfully image various tissue samples, such as rat tail tendon that required 2-3 × lower laser power than the current state-of-the-art. With further development, image-guided, femtosecond laser microsurgical probes such as this one can enable physicians to achieve the highest level of surgical precision anywhere inside the body.
    Optics Express 05/2011; 19(11):10536-52. DOI:10.1364/OE.19.010536 · 3.53 Impact Factor
  • W. Piyawattanametha, M.J. Schnitzer
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    ABSTRACT: We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm<sup>3</sup>) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a photonic bandgap fiber for laser excitation and large core fiber for fluorescence collection. We demonstrated cortical blood flow imaging in live mice with transverse (Δx) and axial resolutions (Δz) of 1.6 μm and 13.5 μm, respectively.
    Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on; 03/2011

Publication Stats

1k Citations
108.67 Total Impact Points

Institutions

  • 2015
    • King Mongkut's Institute of Technology Ladkrabang
      Krung Thep, Bangkok, Thailand
  • 2010–2014
    • Chulalongkorn University
      • Faculty of Medicine
      Krung Thep, Bangkok, Thailand
  • 2009–2013
    • National Electronics and Computer Technology Center
      Bang Kadi, Pathum Thani, Thailand
  • 2006–2012
    • Stanford University
      • • Department of Pediatrics
      • • Department of Electrical Engineering
      Stanford, CA, United States
  • 2008
    • University of Texas at Austin
      • Department of Mechanical Engineering
      Austin, TX, United States
  • 2007
    • Massachusetts Institute of Technology
      • Department of Electrical Engineering and Computer Science
      Cambridge, Massachusetts, United States
  • 2000–2006
    • University of California, Los Angeles
      • Department of Electrical Engineering
      Los Angeles, California, United States
  • 2004
    • HRL Laboratories, LLC
      Malibu, California, United States