M Puar

Publications (5)9.34 Total impact

• Article: Macrolactams: a novel class of antifungal antibiotics produced by Actinomadura spp. SCC 1776 and SCC 1777.

  V Hegde, M Patel, A Horan, V Gullo, J Marquez, I Gunnarsson, F Gentile, D Loebenberg, A King, M Puar
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  ABSTRACT: Three novel antifungal antibiotics, Sch 38518, Sch 39185 and Sch 38516 were isolated from the fermentation broths of two actinomycetes identified by chemical, morphological and physiological analysis as a new species of Actinomadura. The compounds were isolated from broth by solvent extraction and purified by silica gel chromatography. Physico-chemical properties, mass spectral analysis, IR and UV suggested the compounds were similar. Sch 38518 and Sch 39185 have a molecular formula of C25H48N2O5. 1H NMR, 13C NMR and hydrolysis indicated the aglycones were identical, however the compounds differed in containing isomeric sugar moieties. Sch 38518 contains mycosamine while Sch 39185 contains 3,6-dideoxy-3-amino-L-talopyranose. Sch 38516 has a molecular formula of C24H46N2O5 and is a lower homolog of Sch 39185. The three compounds, Sch 38518 (1), Sch 39185 (2), and Sch 38516 (3) exhibit similar activity against Candida spp. with geometric mean MICs of 1.81, 2.00 and 0.91 micrograms/ml, respectively.
  The Journal of Antibiotics 06/1992; 45(5):624-32. · 1.65 Impact Factor
• Article: Macrolactams: two novel homologous series of compounds produced by Actinomadura sp. SCC 1778.

  R Cooper, I Truumees, R Yarborough, D Loebenberg, J Marquez, A Horan, M Patel, V Gullo, M Puar, B Pramanik
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  ABSTRACT: Eight antifungal compounds were identified from the fermentation of Actinomadura sp. SCC 1778. This culture produces four homologous compounds (C22H42N2O5 approximately C25H48N2O5) containing the sugar, mycosamine, and four homologous compounds (C22H42N2O5 approximately C25H48N2O5) containing the sugar, 3,6-dideoxy-3-amino-L-talopyranose. Five of the compounds identified were novel macrolactams. All these compounds exhibit antifungal activity against Candida spp. with geometric mean MICs ranging from approximately 1.0 micrograms/ml for the higher homologs to 30 micrograms/ml for the lower homologs.
  The Journal of Antibiotics 06/1992; 45(5):633-8. · 1.65 Impact Factor
• Article: Sch 42137, a novel antifungal antibiotic from an Actinoplanes sp. Fermentation, isolation, structure and biological properties.

  R Cooper, I Truumees, I Gunnarsson, D Loebenberg, A Horan, J Marquez, M Patel, V Gullo, M Puar, P Das
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  ABSTRACT: A novel polycyclic xanthone, Sch 42137, related to the albofungin family of compounds was isolated from culture broth. Its structure was determined by detailed spectroscopic studies and comparison of circular dichroic studies to related compounds albofungin and simaomicin. Sch 42137 exhibited MIC values less than 0.125 micrograms/ml against yeasts and dermatophytes. Details are presented herein.
  The Journal of Antibiotics 05/1992; 45(4):444-53. · 1.65 Impact Factor
• Article: A novel antifungal from an Actinomadurae with preferential activity against the mycelial phase ofCandida albicans

  V. Gullo, I. Gunnarsson, V. Hegde, A. Horan, D. Loebenberg, J. Marquez, M. Patel, M. Puar, J. Schwartz
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  ABSTRACT: Sch 40873, a novel antifungal compound isolated from the fermentation broth of anActinomadura spp. was discovered in an assay designed to detect compounds with preferential activity against the invasive mycelial form ofCandida albicans. The geometric mean MIC of Sch 40873 against sevenCandida spp. in Sabouraud dextrose broth (yeast phase) was 58 g/ml and in Eagles minimum essential medium (mycelial phase) was g/ml. Sch 40873 demonstrated slight in vivo topical activity in a hamster vaginal model.
  Journal of Industrial Microbiology and Biotechnology 01/1991; 8(1):65-68. · 2.73 Impact Factor
• Article: Saramycetin, a thiazolyl peptide from a Streptomyces sp.: chemical characterization and molecular weight determination.

  R Cooper, I Truumees, T Barrett, M Patel, J Schwartz, M Puar, P Das, B Pramanik
  The Journal of Antibiotics 08/1990; 43(7):897-900. · 1.65 Impact Factor