Publications (7)2.08 Total impact
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Article: Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis.
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ABSTRACT: Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.Veterinary Immunology and Immunopathology 11/2011; 145(1-2):120-8. · 2.08 Impact Factor -
Article: Acute phase protein response in cows with staphylococcus aureus subclinical mastitis
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ABSTRACT: Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs) with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30) were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group), second group were cows with one quarter infected with Staph. aureus (SaQ1) and third group were cows with two quarters infected (SaQ2). Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA) and somatic cell count (SCC). Serum samples where analyzed for SAA, haptoglobin (Hp), ceruloplasmin (Cp) and albumin concentration. Also, complete blood count (CBC) was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved.Acta Veterinaria. 01/2010; -
Article: Cultivation of hamster bone marrow haematopoietic stem and progenitor cells
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ABSTRACT: Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia.Acta Veterinaria. 01/2010; -
Article: A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition
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ABSTRACT: The paper presents the results of several health status parameters of sheep bred in the area exposed to depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. The blood samples of sheep were collected randomly in the region of Bujanovac, in 2004. Complete blood count was performed according to standard laboratory procedures. Concentration of red blood cells malondialdehyde (RBC MDA) and activity of erythrocyte superoxid dismutase (SOD) were determined spectrophotometrically, while the functional activity of leukocytes was investigated by NBT reduction and adhesion test. The results of complete red blood cells count indicated normocytic hypochromic anaemia. The total number of leukocytes and the differential leukocytes count were within the physiological range. Although the leukocytes adhesiveness was not changed in DU exposed animals, their increased NBT reduction revealed stimulated leukocytes' oxidative burst. This finding, together with significantly increased RBC MDA concentrations, as well as the activity of RBC antioxidant SOD, point to the existence of oxidative stress. Although the results indicated that animals were under oxidative stress, still there are no conclusive data that it was due to the exposure of DU that entered the environment during military operations.Acta Veterinaria. 01/2009; -
Article: Expression of α-SMA, desmin and vimentin in canine liver with fibrosis
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ABSTRACT: In this work, 36 canine livers with fibrosis were examined. Based on the degree of fibrosis, the livers were classified into three groups: 1- mild portal fibrosis; 2- moderate portal fibrosis with mild periportal and septal fibrosis; 3- severe portal fibrosis with marked periportal and septal fibrosis or cirrhosis. Normal liver sections obtained from five dogs with no evidence of infectious, neoplastic, or cardiovascular diseases were used as controls. The degree of fibrosis was evaluated on liver sections stained according to Masson's trichrome method. It was proved using an immunohistochemical method that HSC and periductal myofibroblasts are immunoreactive to desmin, vimentin and α-SMA. Desmin, vimentin and α-SMA positive cells in the portal triad were significantly more numerous in dogs with fibrosis of the third degree than in the controls or all other examined livers. The density of α- SMA, vimentin and desmin-positive cells in the portal triad was in correlation with the degree of fibrosis, which cannot be said for HSC. These results suggest that portal myofibroblasts have a significant role in the process of liver fibrosis in dogs.Acta Veterinaria. 01/2009; -
Article: Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran
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ABSTRACT: Serum iron concentration and iron saturation of transferrin (Trf) are measures of body iron stores after administration of iron supplements. In clinical and experimental research, the complex determination of Trf was replaced by the simple determination of total iron binding capacity (TIBC). The objective of this work was to define if TIBC could be an adequate measure for Trf in neonatal piglets after i.m. iron administration. Treated piglets received 150 mg of iron-dextran i.m. the first day of life, and were compared to the untreated control group. Prior to iron administration, as well as on days 2, 8 and 12 after iron administration, serum iron and TIBC concentration were analyzed by an automatized chemical analyzer and Trf was determined by densitometry of electrophoretic strips. Our results show that regardless of iron treatment, TIBC is not a measure of Trf concentration in neonatal piglets two days after birth. At day 8 of their life a high correlation coefficient of these two parameters was established in non-treated animals, while in iron-treated piglets the same correlation was established 12 days after iron treatment. Thus, we suggest that in neonatal piglets, TIBC could be used as a measure of Trf concentration only 12 days after i.m. iron treatment.Acta Veterinaria. 01/2006; -
Article: Pirotski kačkavalj
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ABSTRACT: In the Stara planina region, around county Pirot, in the last 100 years the production of kachkaval cheese, known in the wide region as Pirotski kachkaval, has been developed. Stara Planina is well known by its pastures for sheep and cattle, high height above the sea level and specific climate. Pirotski kachkaval was produced on Stara planina in the period of sheep pasture, May - September. It was manufactured traditionally by soaking of mature cheese (baskija) and hand making a wheel of cheese. Ripening and salting of cheese in the climate conditions of Stara Planina, gave recognizable taste of Pirotski kachkaval. The necessity of protection of traditionally manufactured Pirotski kachkaval cheese, brought up the investigations which proved that all characteristics of cheese produced in traditional way, are kept, pointing out that this unique procedure satisfies demands for PDO (Protected Denomination of Origin) mark.Mljekarstvo/Dairy (urednik@hmu.hr); Vol.55 No.3.
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2011
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University of Belgrade
- Faculty of Veterinary Medicine
Belgrade, SE, Serbia
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