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ABSTRACT: Marine bacterium Catenovulum agarivorans YM01(T) can produce highly thermostable agarases. The draft genome of YM01(T) is about 5.36 Mb and harbors approximately 4,913 genes, including 15 agarase (2 α-agarase and 13 β-agarase)-encoding genes, which will provide references to functional characterization of various agarases from marine bacteria.
Journal of bacteriology 10/2012; 194(19):5484. · 3.94 Impact Factor
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ABSTRACT: A novel bacterial strain, designated MY15T, was isolated from a saliva sample taken from a student during a teaching experiment in China. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was most closely related to Myroides marinus JS-08T, Myroides odoratimimus LMG 4029T and Myroides profundi D25T with 96.5 %, 96.3 % and 96.1 % gene sequence similarities, respectively, demonstrating that the novel strain belonged to the genus Myroides. Strain MY15T formed pale yellow colonies that turned to brown on Luria-Bertani (LB) agar and that gave off a characteristic fruity odour. Cells were Gram-staining-negative, rod-shaped and non-motile. The new isolate contained menaquinone 6 (MK-6) as the major respiratory quinone and C15:0 iso (51.2 %), C17:0 iso 3-OH (12.9 %) and C13:0 iso (10.5 %) as the dominant fatty acids. The G+C content of the DNA was 34.3 mol%. On the basis of this study, based on a polyphasic taxonomic approach, strain MY15T (=DSM 23313T=LMG 25566T) represents a novel species of the genus Myroides, for which the name Myroides phaeus sp. nov. is proposed. Emended descriptions of the genus Myroides and of the species M. profundi and M. marinus are also given.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2011; 62(Pt 4):770-5. · 2.11 Impact Factor
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ABSTRACT: A novel Gram-negative, non-motile bacterium, designated ZXM137(T), was isolated from seawater collected from a coastal region of Qingdao, China, during a massive green algae (Enteromorpha prolifera) bloom. Strain ZXM137(T) was strictly aerobic and did not accumulate poly-β-hydroxybutyrate. Growth occurred with 0.5-11.0% (w/v) NaCl, at pH 6-9 (optimum of pH 7) and at 4-45 °C (optimum at 28 °C). It contained Q-10 as the predominant ubiquinone and the major polar lipids were phosphatidylglycerol, phospholipids, and an unidentified aminolipid and lipid. The major cellular fatty acids of strain ZXM137(T) were C(18:1)ω7c, C(18:1)ω6c and 11-methyl C(18:1)ω7c. The DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZXM137(T) formed a distinct evolutionary lineage within the Roseobacter group in the class Alphaproteobacteria. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain ZXM137(T) represents a novel species in a new genus, for which the name Huaishuia halophila gen. nov., sp. nov. is proposed; the type strain is ZXM137(T) (=CGMCC 1.8891(T)=LMG 24854(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2011; 62(Pt 1):223-8. · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, non-motile, non-sporulating bacterial strain, designated LYX05(T), was isolated from coastal sediment of Qingdao, China, on the coast of the Yellow Sea. Strain LYX05(T) was aerobic and heterotrophic. The strain grew optimally at 37 °C and pH 7.5 and in the presence of 2% (w/v) NaCl. Colonies were 1-2 mm in diameter, circular, reddish orange and shiny with entire edges on marine agar medium. Cells were rods (0.3-0.5 µm wide and 0.8-1.6 µm long). The dominant fatty acids were iso-C(15:0) (40.82%) and C(16:0) (10.45%). The DNA G+C content was 42.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LYX05(T) was phylogenetically related to the members of the genus Algoriphagus and the closest relative was Algoriphagus hitonicola 7-UAH(T) (95.8% 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain LYX05(T) was considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus faecimaris sp. nov. is proposed. The type strain is LYX05(T) (=JCM 16561(T) =DSM 23095(T) =LMG 25474(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2011; 61(Pt 12):2856-60. · 2.11 Impact Factor
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ABSTRACT: A novel Gram-negative, strictly aerobic, agar-hydrolysing bacterium, designated YM01(T), was isolated from seawater samples collected from the Yellow Sea (coastal region of Qingdao, PR China). Cells were rod-shaped, peritrichously flagellated and formed long chains end-to-end. The isolate had an absolute requirement for Na(+) ions, but not seawater, for growth and grew optimally at about 28 °C, in 2% NaCl and at pH 8.0-9.0. The isolate could not be cultured in marine broth 2216, but grew well on marine agar 2216. YM01(T) was able to hydrolyse cellulose, starch, aesculin and Tween 80, but not egg yolk, gelatin, urea or casein. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing only 88.4-91.0% sequence similarity to its closest neighbours, including members of the genera Glaciecola (88.4-91.0%), Alteromonas (88.7-89.6%), Aestuariibacter (89.3-90.4%), Salinimonas (89.0%), Bowmanella (90.1-90.3%) and Agarivorans (88.5-89.9%). Phylogenetic analyses demonstrated that strain YM01(T) formed a distinct clade closely related to species of the family Alteromonadaceae within the group of Alteromonas-like gammaproteobacteria. It contained menaquinone MK-7 as the predominant isoprenoid quinone and C(16:0) (38.3%), C(16:1)ω7c and/or iso-C(15:0) 2-OH (29.0%), C(18:1)ω7c (9.3%) and C(10:0) 3-OH (8.2%) as major cellular fatty acids. Phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid were the major phospholipid constituents. The DNA G+C content was 44.8 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain YM01(T) is considered to represent a novel species in a new genus in the Gammaproteobacteria, for which the name Catenovulum agarivorans gen. nov., sp. nov. is proposed; the type strain of Catenovulum agarivorans is YM01(T) (=CGMCC 1.10245(T) =DSM 23111(T) =JCM 16580(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2011; 61(Pt 12):2866-73. · 2.11 Impact Factor
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ABSTRACT: A marine antagonistic bacterium, JG1, was isolated from rearing water of healthy turbot (Scophthalmus maximus) in Qingdao, China. Strain JG1 was Gram-negative, straight rod and motile by polar flagella. The colony, when cultured for 24 h under room temperature, produced a yellow pigment. On the basis of morphological, physiological and biochemical characteristics, along with 16S rDNA sequence analysis, JG1 was identified as Pseudoalteromonas flavipulchra. JG1 had good inhibitory effects on several bacterial pathogens of aquaculture in the genus Vibrio (V. anguillarum, V. alginolyticus, V. campbellii, V. harveyi, V. mimicus, V. parahaemolyticus and V. tubiashii) and Aeromonas (A. hydrophila and A. salmonicida). No mortality occurred 14 days after zebra fish and 7 days after mantis shrimp were intraperitoneally injected with JG1 at 106 CFU per animal, and 7 days after scallop and clam were immersed in JG1 at 107 CFU mL−1. A good antagonistic effect on several bacterial pathogens and nontoxicity to the above-mentioned animals make JG1 a potential probiotic in aquaculture. In addition, a fast detection technique based on polymerase chain reaction amplification of the gyrB gene was established to allow us to determine the fate of JG1 when it is applied in aquaculture in the future.
Aquaculture Research 11/2010; 41(12):1867 - 1874. · 1.20 Impact Factor
