Are you Shu-qin Zhang?

Claim your profile

Publications (2)0 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the role of pulmonary intravascular macrophages (PIMs) in the pathogenesis of acute lung injury (ALI) due to infection. Porcine pulmonary blood vessels were flushed by modified Morton method, and PIMs were isolated and cultured. The adhered PIMs were collected with adhesion method and incubated in RPMI 1640 medium. They were challenged with lipopolysaccharide (LPS, 10 mg/L). The activity of interleukin-1β (IL-1β), and contents of IL 6 and IL 8 in the culture supernatant were measured by method of thymocyte proliferation and enzyme linked immunoadsorbent assay (ELISA). The released contents of IL-1β, IL-6 and IL-8 from PIMs were increased significantly compared with those before LPS challenge , and they peaked at 2 hours [IL-1β activity: (10 400 ± 2 389) scintillant count/min], 4 hours [IL-6 content: (0.80 ± 0.36) μg/L], and 6 hours [IL-8 content: (4.94 ± 1.19) μg/L ] after LPS challenge , and the differences were significant compared with hose before LPS challenge [IL-1β activity: (213 ± 85) scintillant count/min, IL-6 content: (0.27 ± 0.12) μg/L, IL-8 content: (1.84 ± 0.53) μg/L, all P <0.01]. Among the cytokines released from PIMs after LPS challenge , the increase in IL-1β occurred earlier in comparison with that of IL-6 and IL-8, suggesting that the former might play an important role at the early stage of ALI; on the other hand, though the increase in IL-6 and IL-8 contents occurred later than that of IL- 1β but it lasted for a longer duration, suggesting that they might be associated with the advancement of ALI. The Results also suggested that interaction of these cytokines played a more important role in the pathogenesis of ALI.
    Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 03/2011; 23(3):176-8.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the roles of pulmonary intravascular macrophages (PIMs) in the pathogenesis of infective acute lung injury (ALI). Porcine pulmonary blood vessels were flushed by modified Morton's method, PIMs were isolated with adhesion method and incubated in RPMI 1640 medium. They were stimulated with lipopolysaccharide (LPS, 10 mg/L). The contents of interleukin-6 (IL-6), IL-8 and tumor necrosis factor-alpha (TNF-alpha) in the culture supernatants were respectively measured by enzyme linked immunoadsorbent assay (EILSA). The release of TNF-alpha, IL-6 and IL-8 by PIMs was increased significantly as compared with the levels before stimulation by LPS, peaking at 1, 4, and 6 hours after LPS stimulation, respectively. The differences were significant (all P<0.01). Among the cytokines released by PIMs after LPS challenge, the increase in TNF-alpha content occurs earlier in comparison with that of IL-6 and IL-8, suggesting that the former may play an important role at the early stage of ALI. On the other hand, the increase in IL-6 and IL-8 contents is later than that of TNF-alpha and lasts for a longer time, suggesting that they may be associated with the development of ALI. The results also suggest that interaction of these cytokines is more important in the pathogenesis of ALI.
    Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 04/2006; 18(3):136-8.