[show abstract][hide abstract] ABSTRACT: The effects of the composition of ceolomic fluid and sperm function are not well understood inteleostean fish species. The aim of the present study was to determine the concentration of the majorinorganic ions (Na+, K+, Ca2++, Mg2+, Cl-), organic composition (glucose and total protein) and pH ofovarian fluid in Prussian carp Carassius gibelio (Bloch, 1782), and to determine if the composition ofthese fluids influences sperm motility trait (percentage and duration of motility). In this purpose, spermmotility traits in 3 medium (ovarian fluid, fresh water + ovarian fluid and fresh water alone) were tested.The ovarian fluid was composed of sodium 133.1 ± 3.8 mM L-1, potassium 2.4 ± 0.28 mM L-1, calcium0.56 ± 0.28 mM L-1, magnesium 0.65 ± 0.23 mM L-1, chloride 135.4 ± 3.92 mM L-1, total protein3.3 ± 0.46 gdL-1, glucose 3.57 ± 0.48 mM L-1 and pH 8.22 ± 2.9. The results suggested that spermmovement duration was significantly higher in ovarian fluid than fresh water and fresh water + ovarianfluid during spawning season. The percentage of motile spermatozoa did not differ significantly amongovarian fluid, fresh water + ovarian fluid and fresh water in spawning periods. As a conclusion, theresults of this study recommend the use of ovarian fluid, it is useful as an activation medium toimproving sperm motility parameters of Prussian carp.
[show abstract][hide abstract] ABSTRACT: The objectives of the present study were to determine the relationships between chemicalscompositions of seminal plasma with sperm motility traits in Prussian carp, Carassius gibelio (Bloch,1782). There were significant positive correlations between sperm movment duration and Ca+2 of semen.Also, a significant positive relationship was found between percentage of motile spermatozoa and Ca+2 ofsemen. On the other hand, Na+, Cl- and pH correlated negatively with sperm movment duration.Understanding of such correlations can be useful to evaluation of sperm quality and make media(extender) for dilution of semen and improving sperm motility parameters of Prussian carp.
[show abstract][hide abstract] ABSTRACT: In order to study the effects of storage media and time of storage on the viability ofunfertilized eggs of Prussian carp, Carassius gibelio (Bloch, 1782), the ova of this fish, outside the ovary(ex situ storage), were stored in Dettlaff extender (111.3 NaCl + 3.3 KCl + 2.1 CaCl2 + 23.8 NaHCO3)and PCACF (Prussian carp Artificial Coelomic Fluid) medium at 4°C and 21°C for 20, 30, 60 and 120 min.For mimicking of ovarian fluid and designing the PCACF medium, obtained ova from 15 female, werefiltered and coelomic fluid was separated for chemical composition analysis of ovarian fluid and thePC ACF medium was formulated. This study was done with 16 treatments and 1 control group in 3replicate. The results shown that maintain duration and storage medium had significant effect on eyedembryos rate, hatching success (P<0.05), but their effect on malformation of larvae was not significant(P>0.05). Also, temperature had significant effects on percentage of eyed embryos and hatching rate(P<0.05) but no significant effects on number of malformed larvae (P>0.05). Eyed embryo and hatchingrates decreased with increasing time. Interactions between time of storage and storage medium andtemperature and the resulting effects on eye embryos and hatching rates were significant (P<0.05).Highest eyed embryo and hatching rate between treatments was observed in PCACF, 20 min storage and4 °C (PCACF + 20 min + 4 °C) and lowest eyed embryo and hatching rates was observed in Dettlaff +120 min + 21 °C.
[show abstract][hide abstract] ABSTRACT: The aim of this study was to evaluate the influence of L-ascorbic acid (AA) in three levels (0,100, 1000 and 2000 mg L-1) on eyed egg and hatching rate, growth and viability of larva, and larvaltolerance against high temperature stress of Prussian carp Carassius gibelio (Bloch, 1782). The fertilizedeggs were placed in water containing different levels of AA for 3 h. The percentage of eyed egg andhatching were measured after 2 and 3 days respectively. After larva absorbed their yolk sac, half ofthem were challenged by high temperature (30°C) and the others were reared for 45 days and growthfactors and survival were calculated. The result shown that the highest eyed egg and hatching rate werein 2000 mg L-1 and had significantly differece with other treatments (P<0.05). The significant differencesin larval tolerance against high temperature stress were observed in 1000 and 2000 mg L-1 compared to0 and 100 mg L-1 treatments. No significant differences were observed between growth parameters oftreatment batches (P>0.05). Viability was different between experimental groups, but it was notsignificant between 0 and 100 mg L-1. According to our results when broodstocks of Prussian carp do nothave enough vitamin C in their ovaries, immersion of fertilized eggs in 2000 mg L-1 of AA may bebeneficial.
[show abstract][hide abstract] ABSTRACT: A 10 weeks growth experiment was conducted to determine the effects of dietary vitamin C,E and highly unsaturated fatty acid on growth and survival of goldfish (carassius auratus) with initialweight of 0.69±0.12 gr. They were fed with ten experimental diets having different level of vitamin E(50, 100, 1000 mg kg diet-1), C (100, 1000 mg kg diet-1), HUFA (highly unsaturated fatty acid): thetest treatments were as follow: C100+E1000-HUFA, C1000+HUFA, C100+HUFA, C100+E1000+HUFA,E100+HUFA, E50+HUFA, C1000-HUFA, C100+HUFA,-C-E+HUFA,-C-E-HUFA. Final weight, specificgrowth rate, feed conversion rate and condition factor were evaluated at the end of experimentalperiod. Final weight, specific growth rate were higher in fish fed the diet with C100+E1000+HUFA. Thelowest final weight and specific growth rate were observed in-C –E-HUFA.Whereas food conversion rate(FCR) and condition factor (K) were not significantly different between groups. These results indicatethat the diet with C100+E1000+ HUFA can effect some factors growth in goldfish, Carassius auratus.