S Seeber

Universitätsklinikum Erlangen, Erlangen, Bavaria, Germany

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Publications (354)1185.62 Total impact

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    ABSTRACT: Background: we have already reported cumulative probability (prob) of brain relapse as first site of failure within our randomized trial that investigated TRIMODALITY + prophylactic cranial irradiation (PCI) versus SURG + RTx alone in operable IIIA NSCLC (Pöttgen, JCO 2007). Here we report LTS for these arms, look for exploratory subgroups and investigate competing risks. Methods: Pts with histopathologically (mediastinoscopy) proven operable IIIA (1-2 LN involved, no clinical N2, no bulky/extranodal disease, central T3N0-1, WHO 0,1) NSCLC were stratified (TN-group, center) and randomized. Arm B received three CTx cycles cis (60 mg/m2 d 1+7 or 8) and eto (150 mg/m2 d 3,4,5) qd 22. This was followed by cc CTx/RTx including cis 50 mg/m2 d 2 + 9 and eto 100 mg/m2 d 4-6 cc with 45 Gy (1.5 Gy bid). 3-5 weeks after end of RTx SURG was performed if possible and PCI given thereafter. Arm A had local treatment alone (SURG + RTx) and no PCI. Results: Pts accrual 1/95 to 10/01; eligible 106/112 randomized; Pts characteristics: gender m 90; f 16; age median 57 (37-71); PS 0-1; histo: adeno (ADC) 35; squamous cell (SCC) 50; large cell (LCC) 16; ADSCC 5 TN-groups: T3N0-1 6 T1-2N2 83 T3N2 17. Med S of pts still alive on f-up: 130 mo. LT exploratory OS analysis (2-y-OS %; 5-y-OS %, 10-y-OS %); B: 54.6 20.0 12.5; A: 44.2 23.5 10.0 (ns log rank, Wilcoxon); PFS: B: 36.4 14.6 12.7 A: 29.4 17.3 10.4 (p = 0.30/0.14 log rank/Wilcoxon) Disease-specific survival: B: 40.5 25.0 25.0 A: 32.5 23.1 18.5 (p = 0.16/0.059 log rank/Wilcoxon), Cumulative 10-y prob %: non-disease-related events (tox, comorbidity, second cancer): B: 56.3 A: 67.7 (ns); brain relapse as first site of failure: B: 16.6 A: 30.4 (p = 0.059/0.020 log rank/Wilcoxon). Conclusions: LTS after Tx in stage IIIA is significantly hampered by cumulative competing risks (toxicities, comorbidities, second cancers) regardless of Tx intensity. Initial pts selection based on comorbidity profiles may be most important in improving LTS with aggressive protocols based on TRIMODALITY (+/- PCI).
    Journal of Clinical Oncology 01/2011; 29(Suppl.):abstr 7042. · 18.43 Impact Factor
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    ABSTRACT: The strychnine-sensitive glycine receptor (GlyR) is a ligand-gated chloride channel and a member of the superfamily of cysteine loop (Cys-loop) neurotransmitter receptors, which also comprises the nicotinic acetylcholine receptor (nAChR). Within the extracellular domain (ECD), the eponymous Cys-loop harbors two conserved cysteines, assumed to be linked by a superfamily-specific disulfide bond. The GlyR ECD carries three additional cysteine residues, two are predicted to form a second, GlyR-specific bond. The configuration of none of the cysteines of GlyR, however, had been determined directly. Based on a crystal structure of the nAChRalpha1 ECD, we generated a model of the human GlyRalpha1 where close proximity of the respective cysteines was consistent with the formation of both the Cys-loop and the GlyR-specific disulfide bonds. To identify native disulfide bonds, the GlyRalpha1 ECD was heterologously expressed and refolded under oxidative conditions. By matrix-assisted laser desorption ionization time-of-flight mass spectrometry, we detected tryptic fragments of the ECD indicative of disulfide bond formation for both pairs of cysteines, as proposed by modeling. The identity of tryptic fragments was confirmed using chemical modification of cysteine and lysine residues. As evident from circular dichroism spectroscopy, mutagenesis of single cysteines did not impair refolding of the ECD in vitro, whereas it led to partial or complete intracellular retention and consequently to a loss of function of full-length GlyR subunits in human embryonic kidney 293 cells. Our results indicate that the GlyR ECD forms both a Cys-loop and a GlyR-specific disulfide bond. In addition, cysteine residues appear to be important for protein maturation in vivo.
    Journal of Biological Chemistry 10/2009; 284(52):36128-36. DOI:10.1074/jbc.M109.043448 · 4.57 Impact Factor
  • O Kloke · N Niederle · B Opalka · I Hawig · S Seeber · R Becher ·
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    ABSTRACT: To evaluate the long-term impact of the reduction of Philadelphia chromosome (Ph)-positive metaphases by treatment of chronic myelogenous leukaemia (CML) with interferon (IFN) alpha, we examined the outcome of 62 patients who had been enrolled between 1984 and 1990 into 2 IFN trials at our institution. As best cytogenetic response, 9 patients had achieved a complete remission and an additional 9 patients a partial remission. The remaining 44 patients had obtained either a minimal (n=29) or no cytogenetic response (n=15). Of the total of 62 patients, 9 were still on schedule and responsive to IFN in January 1995, including 7 patients in ongoing complete cytogenetic remission. The overall 5-year survival rate after a median follow-up from diagnosis of 51 months (range 3-102 months) was 62% and the median survival was reached at month 87. The effect of cytogenetic remission on survival was examined by "landmark" studies showing a significant survival advantage for patients with karyotype responses. In conclusion, in the patients studied, cytogenetic improvement was found to translate into improved survival expectancy. Long-term control by IFN alpha of CML, however, was restricted to a small minority of patients, predominantly to those attaining a complete suppression of the leukaemic cell clone as judged by cytogenetic criteria.
    European Journal Of Haematology 01/2009; 56(1-2):78-81. DOI:10.1111/j.1600-0609.1996.tb00299.x · 2.07 Impact Factor

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    ABSTRACT: Anthracyline-based chemotherapy is the treatment of choice for patients with aggressive B-cell non-Hodgkin's lymphoma (NHL). However, anthracyclines have been associated with long-term cardiac toxicity. We conducted a study using a sequential combination chemotherapy with a reduced cumulative dose of anthracyclines in younger patients with good-prognosis aggressive NHL. Chemotherapy consisted of one cycle of vincristine, ifosfamide, etoposide, and dexamethasone, followed by three cycles of epirubicin, cyclophosphamide, vincristine, and dexamethasone, and a fifth cycle containing carboplatin, etoposide, and dexamethasone. 86 patients were treated, 65 without and 21 with additional rituximab. Consolidating involved-field irradiation was applied in patients with stage I/II, bulky disease, or localized residual lymphoma. Complete and partial remissions were achieved in 67 and 27% of patients, respectively, and the 3-year event-free and overall survival estimates were 75 and 87%. The survival estimates were substantially better in patients who received rituximab. Main toxicity was grade 3/4 leukocytopenia in 89% patients with neutropenic fever in 30%. Two patients died of septic shock. The treatment appears to be effective in this group of patients. The hematological toxicities, particularly after the first and fifth cycle, require the use of G-CSF and/or a dose reduction in selected patients.
    Journal of Cancer Research and Clinical Oncology 09/2008; 135(3):459-66. DOI:10.1007/s00432-008-0467-2 · 3.08 Impact Factor
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    ABSTRACT: In this study parameters relevant for glaucoma in DBA/2J (D2J) mice were compared with those in age-matched DBA/2J-Rj (D2Rj) mice, to challenge the postulated role of D2J mice as a model for secondary high-tension glaucoma. Genotyping for three known short nucleotide polymorphisms (SNPs) in the Tyrp1 gene and the Gpnmb gene by MALDI-TOF-MS and immunohistochemical staining for Gpnmb was performed in D2J and D2Rj mice. Twelve C57Bl/6 (B6), 8 D2Rj, and 11 D2J mice between 1 and 4 months of age were screened qualitatively and quantitatively for morphologic differences within the anterior eye segment. The IOP progression of 25 D2Rj and 18 D2J mice were investigated between 4 to 10.5 months after birth. At the end of this study, in 10 randomly selected individuals of each D2J and D2Rj cohort, correlation of IOP progression and optic nerve damage were determined in each eye. D2J and D2Rj strains were homozygous for both Tyrp 1 amino acid substitutions, so far only described in D2J mice. The Gpnmb(R150X) point mutation present in D2J mice was not detected in D2Rj. Accordingly, immunoreactivity (IR) for Gpnmb was present only in D2Rj and B6 eyes, but not in D2J. Compared with B6, both DBA/2 mice (D2) showed a significantly narrowed chamber angle caused by an anteriorly displaced ciliary body. IOP measurements showed an average IOP of approximately 14 mm Hg between age 4 and 7 months in D2Rj, which decreased to approximately 11 mm Hg in the period from 8 to 10.5 months. In D2J the average IOP showed a steady increase in the observed period from 4 to 10.5 months (from 8.65 to 15.58 mm Hg). Individuals with IOP peaks up to 30 mm Hg were detected in D2Rj, but none of these mice showed signs of an optic neuropathy after 10.5 months. In contrast, 30% of the investigated D2J mice at the age of 10.5 months showed a severe optic neuropathy. Individual data analyses, however, showed no significant correlation between elevated IOP and glaucomatous changes within the D2J population. Individual correlations of IOP course with axon loss in the single eyes confirmed that in D2J mice, hypertension is not the only causative factor in glaucomatous optic neuropathy. For further investigations on the pathogenesis of glaucoma in D2J mice, the D2Rj strain without a Gpnmb(R150X) mutation and without glaucomatous changes, but with individual IOP elevation, can be used as an interstrain control for D2J.
    Investigative Ophthalmology &amp Visual Science 03/2008; 49(2):613-21. DOI:10.1167/iovs.07-0745 · 3.40 Impact Factor
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    S. Seeber · A. Welt ·
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    ABSTRACT: Eine vielfältige klinische und zellbiologisch-experimentelle Forschungslandschaft trägt dazu bei, dass gerade beim metastasierten Mammakarzinom die zunehmenden therapeutischen Alternativen einer medikamentösen Behandlung nicht nur einem steten Wandel unterworfen sind, sondern glücklicherweise auch zu einer schrittweisen Verbesserung der Langzeitergebnisse führen [1]. Wie bei der einzelnen Patientin zu verfahren ist, wird durch die onkologischen Therapeuten oft recht unterschiedlich beurteilt. Auch aus Sicht der Autoren sind bei den zahlreichen unterschiedlichen Situationen eines Langzeitverlaufs die Behandlungsmöglichkeiten heterogen. Diese können durch die gegenwärtige Studienmedizin nur teilweise abgebildet werden [2]. Deshalb sind Vorgaben durch vereinfachende Leitlinien von Fachgesellschaften auch nicht immer befriedigend.
    best practice onkologie 01/2008; 3(3). DOI:10.1007/s11654-008-0047-6

  • EJC Supplements 09/2007; 5(4):390-390. DOI:10.1016/S1359-6349(07)71436-2 · 9.39 Impact Factor
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    ABSTRACT: Spreading transmissible spongiform encephalopathies (TSE) have been widely attributed to transmission by ingestion of mammalian central nervous system (CNS) tissue. Reliable exclusion of this epidemiological important route of transmission relies on an effective surveillance of food contamination. Here, myelin proteolipid protein (PLP) is identified as a specific and largely heat-resistant marker for detection of food contaminations by CNS tissue. PLP is a component of oligodendritic glial sheaths of neuronal processes that is specifically expressed in the CNS. A highly selective polyclonal antibody was developed directed against an epitope present in the full-length PLP protein, but absent from the developmentally regulated splice variant DM-20. In combination with a hydrophobic extraction of PLP from tissue samples, the antibody reliably detected PLP from spinal cord, cerebellum, and cortex of different mammalian species. Consistent with earlier reports on PLP expression, no cross-reactivity was observed with peripheral nerve or extraneural tissue, except for a very faint signal obtained with heart. When applied to an artificial CNS contamination present in sausages, the antibody reliably detected a low concentration (1%) of the contaminant. Application of heat, as used during conventional sausage manufacturing, led to a predominant alteration of arginine residues in the PLP protein and a partial loss of immunoreactivity. In contrast, a stretch of hydrophilic amino acids(112-122) proved to be heat-resistant, preserving the immunogenicity of this PLP epitope during heating. Taken together, the excellent CNS specificity of PLP immunodetection and the presence of a heat-resistant epitope have permitted the development of a highly sensitive immunoassay for CNS contamination in routine food control.
    Journal of Agricultural and Food Chemistry 09/2007; 55(17):7114-23. DOI:10.1021/jf0707278 · 2.91 Impact Factor
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    ABSTRACT: Heat treatment of dairy products leads to structural changes of proteins, which can severely decrease the nutritional value [Mauron, J. J. Nutr. Sci. Vitaminol. (Tokyo) 1990, 36 (Suppl. 1), S57-69]. In this study, model solutions of the two main whey proteins, alpha-lactalbumin and beta-lactoglobulin, respectively, were incubated with lactose, and modifications were monitored by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Lactulosyl residues were the most abundant modifications of alpha-lactalbumin and beta-lactoglobulin. Up to four of these adducts were identified on the proteins. Enzymatical digest with endoproteinase AspN prior to mass spectrometric analysis allowed the detection of further modifications and their localization in the amino acid sequence. Most prominent modifications were lactulosyllysine, Nepsilon-carboxymethyllysine, oxidation of lysine to aminoadipic semialdehyde, oxidation of methionine to methionine sulfoxide, cyclization of N-terminal glutamic acid to a pyrrolidone, and oxidation of cysteine or tryptophan. The presence of methionine oxidation was deduced from a control protein that had been oxidized by hydrogen peroxide. These studies establish MALDI-TOF-MS as a reliable tool to monitor chemical modifications of nutritional proteins during food processing.
    Journal of Agricultural and Food Chemistry 08/2007; 55(15):6096-103. DOI:10.1021/jf0705567 · 2.91 Impact Factor
  • S Müller · A Welt · S Seeber · R Kimmig · S Kasimir-Bauer ·

    Senologie - Zeitschrift für Mammadiagnostik und -therapie 06/2007; 4(02). DOI:10.1055/s-2007-982988
  • Scholz M · Buder T · Seeber S · Lütjen-Drecoll E ·

    ARVO`s 2007 Annual Meeting; 05/2007
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    ABSTRACT: Although gene transfer with retroviral vectors has shown distinct clinical success in defined settings, efficient genetic manipulation of hematopoietic progenitor cells remains a challenge. To address this issue we have evaluated different transduction protocols and retroviral constructs in the non-obese diabetes (NOD)/severe combined immunodeficiency disease (SCID) xenograft model. An extended transduction protocol requiring 144 h of in vitro manipulation was compared to a more conventional protocol requiring 96 h only. While pretransplantation analysis of cells transduced with a retroviral vector, expressing the enhanced green fluorescent protein (EGFP) marker gene, demonstrated significantly higher overall transduction rates for the extended protocol (33.6 +/- 2.3 vs. 22.1 +/- 3.8%), EGFP expression in CD34+ cells before transplantation (4.0 +/- 0.9 vs. 11.6 +/- 2.5%), engraftment of human cells in NOD/SCID bone marrow 4 weeks after transplantation (4.5 +/- 1.7 vs. 36.5 +/- 9.4%) and EGFP expression in these cells (0 +/- 0 vs. 11.3 +/- 2.8%) were significantly impaired. When the 96 h protocol was used in combination with the spleen focus forming virus (SFFV)/murine embryonic stem cell (MESV) hybrid vector SFbeta11-EGFP, high transduction rates for CD45+ (41.0 +/- 5.3%) and CD34+ (38.5 +/- 3.7%) cells prior to transplantation, as well as efficient human cell engraftment in NOD/SCID mice 4 weeks after transplantation (32.4 +/- 3.5%), was detected. Transgene expression was observed in B-lymphoid (15.9 +/- 2.0%), myeloid (36.5 +/- 3.5%) and CD34+ cells (10.1 +/- 1.5%). Our data show that CD34+ cells maintained in cytokines for multiple days may differentiate and loose their capacity to contribute to the haematological reconstitution of NOD/SCID mice. In addition, the SFFV/MESV hybrid vector SFbeta11-EGFP allows efficient transduction of and gene expression in haematopoietic progenitor cells.
    Journal of Cancer Research and Clinical Oncology 04/2007; 133(3):199-209. DOI:10.1007/s00432-006-0158-9 · 3.08 Impact Factor
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    ABSTRACT: The following study was conducted to explore patients' information needs and preferences with a special focus on doctor-patient communication. A 62-item questionnaire developed by a multidisciplinary team and validated in a phase-I study was given to breast cancer patients via the Internet (homepage) or in a hard copy version. A total of 617 patients responded, 552 on line and 65 via the hard copy questionnaire. The median age of the on-line group was 47 (21-85) and 55 (40-92) in the hard copy group. Sixty-five per cent of the patients were treated with the intention of achieving a cure and 35% of the patients had metastatic disease. The median length of the consultation communicating the information 'You have breast cancer' was 15 min (0-300). The most effective and patient-relevant source of information about the disease and the treatment options was consultation with the physician (84%). When asked to suggest areas for improvement, patients' most common answers were: more complementary therapies should be offered by the physician (54%); physicians should take more time to explain things (51%); and cooperation between the physicians involved in the patient's care should be improved (39%). The questions most relevant to patients were: 'Am I getting the right therapy?' (89%); 'How many patients with my condition does my doctor treat?' (46%) and 'Can I be enrolled into a trial?' (46%). An independent second opinion centre was desired by 94% of the respondents but only 20% knew of any such resource. This study underlines the need to give patients with breast cancer the full details on treatment options and cancer management. The results provide a suitable basis for a broader interdisciplinary discussion of the patient-physician relationship and should be useful in generating hypotheses for subsequent prospective studies.
    Annals of Oncology 04/2007; 18(3):479-84. DOI:10.1093/annonc/mdl456 · 7.04 Impact Factor
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    ABSTRACT: Drug resistance constitutes a considerable problem in the therapy of acute myeloid leukemia (AML). In order to identify genes which might be related to drug resistance, we retrospectively studied gene expression patterns in blast populations of 14 patients with de novo AML, focusing on known or potential resistance mechanisms against cytosine arabinoside and anthracyclines. Following induction and postremission chemotherapy, 7 patients achieved a complete remission (CR) for more than 1 year, while 7 patients showed blast persistence (BP) after induction and salvage chemotherapy. Gene expression analysis was performed using RNA extracted from archived guanidine extracts and Affymetrix HGU133A gene chips. We utilized the Gene Ontology category Biological Process to select genes implicated in DNA metabolism, nucleoside and nucleotide metabolism and transport, reactive oxygen species metabolism, apoptosis and response to drugs and identified 32 differentially expressed genes. From this functional perspective, we found differences between the CR and BP groups with regard to nucleotide metabolism (PBEF1, G6PD; p = 0.048), apoptosis (TNFAIP3, TNFAIP8, MPO, BCL2A1, BAX, SON, BNIP3L; p = 0.039) and reactive oxygen species metabolism (SOD2, KIAA0179; p = 0.048). However, the attempt to construct a predictive model of chemoresistance failed. BP samples had a 2-fold higher expression of CD34 than CR samples. Thus, our findings are in line with reports describing differences in apoptosis resistance between CD34+ and CD34- blast populations. Taken together, our results suggest that drug resistance in AML is a heterogenous phenomenon that might be better defined by means of disturbed biological processes than by focusing on the alteration of the expression of distinct genes.
    Acta Haematologica 02/2007; 117(1):8-15. DOI:10.1159/000096854 · 1.12 Impact Factor
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    ABSTRACT: High-dose chemotherapy (HD-CT) with autologous stem cell transplantation is considered to be the treatment of choice for relapsed high-grade non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL) patients, but the optimal treatment has not yet been defined. We evaluated a salvage treatment regimen consisting of conventional cycles with ifosfamide, etoposide, cytarabine, and dexamethasone (IVAD) followed by two cycles of HD-CT consisting of cyclophosphamide, melphalan, and etoposide (CMV) with autologous stem cell support in patients with relapsed or refractory NHL (n = 59) and HL (n = 16). Response to IVAD was complete remission (CR) in 16 patients (21%), partial remission (PR) in 39 patients (52%), stable disease (SD) in 18 patients (24%), and progressive disease (PD) in two patients (2.7%). Of 70 patients treated with HD-CT, 41 patients (59%) showed a CR, 20 patients a PR (29%), eight patients a SD (11%), and one patient a PD (1.4%). The 5-yr overall survival for the entire group of patients was 29%, and for patients with NHL and HL 25%, and 38%, respectively. The respective event-free survival probabilities at 5 yr were 22%, 16%, and 31%. Seven treatment-related deaths due to septicemia (three), cardiac arrhythmia (one), pneumonia (one), pneumonitis (one), and toxic epidermal necrolysis (one) were observed. In multivariate analysis, an International Prognostic Index of > or = 2 and resistant disease to first-line chemotherapy were poor independent prognostic factors for the subgroup of patients with NHL. In conclusion, these results indicate that IVAD/CMV is feasible as a salvage therapy for lymphoma patients. This treatment is currently evaluated with the addition of rituximab.
    European Journal Of Haematology 02/2007; 78(2):93-101. DOI:10.1111/j.1600-0609.2006.00796.x · 2.07 Impact Factor
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    Melanie Ulrich · Silke Seeber · Cord-Michael Becker · Ralf Enz ·
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    ABSTRACT: Macromolecular signalling complexes that link neurotransmitter receptors to functionally and structurally associated proteins play an important role in the regulation of neurotransmission. Thus the identification of proteins binding to neurotransmitter receptors describes molecular mechanisms of synaptic signal transduction. To identify interacting proteins of GABA(C) (where GABA is gamma-aminobutyric acid) receptors in the retina, we used antibodies specific for GABA(C) receptor rho1-3 subunits. Analysis of immunoprecipitated proteins by MALDI-TOF MS (matrix-assisted laser-desorption ionization-time-of-flight MS) identified the liver regeneration-related protein 2 that is identical with amino acids 253-813 of the Tax1BP1 (Tax1-binding protein 1). A C-terminal region of Tax1BP1 bound to an intracellular domain of the rho1 subunit, but not to other subunits of GABA(C), GABA(A) or glycine receptors. Confocal laser-scanning microscopy demonstrated co-localization of Tax1BP1 and rho1 in clusters at the cell membrane of transfected cells. Furthermore, Tax1BP1 and GABA(C) receptors were co-expressed in both synaptic layers of the retina, indicating that Tax1BP1 is a component of GABA(C) receptor-containing signal complexes.
    Biochemical Journal 02/2007; 401(2):429-36. DOI:10.1042/BJ20061036 · 4.40 Impact Factor
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    ABSTRACT: Die Möglichkeiten einer systemischen Therapie maligner Erkrankungen - außerhalb der zytostatischen Chemotherapie - sind in den letzten Jahren deutlich erweitert worden. Dazu haben insbesondere neue Erkenntnisse zur molekularen Pathogenese von Tumorerkrankungen beigetragen. Von besonderem Interesse sind Strategien, die in Prozesse der tumorassoziierten Neoangiogenese oder der neoplastischen Wachstumsregulation eingreifen.
    Uroonkologie, 12/2006: pages 55-96;
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    ABSTRACT: Purpose: To define the maximum-tolerated dose (MTD) and to evaluate the dose-limiting toxicities (DLT) of irinotecan, leucovorin and 5-fluorouracil (IF-regimen) in combination with escalated doses of biweekly cisplatin in patients with non-resectable locally advanced or metastatic gastric cancer. Patients and Methods: 29 patients with advanced gastric cancer with no prior chemotherapy were treated at 4 dose-levels (DLs). Irinotecan at a dose of 80 mg/m2 was administered on a weekly-times-six schedule in combination with 500 mg/m2 of leucovorin and 2000 mg/m2 of 5-fluorouracil (24 h infusion) (IF-regimen). The dose of biweekly cisplatin was escalated from 20 mg/m2 to 50 mg/m2. One cycle consisted of 7 weeks. Results: Twenty-seven out of 29 patients were evaluable for toxicity. 68 cycles of IF-cisplatin were administered. The MTD was not reached and the dose of cisplatin was escalated to 50 mg/ m2 every two weeks according to the PFL-protocol. However, diarrhea and neutropenia were dose-limiting toxicities after multiple cycles and were observed with NCI-CTC grade 3 and 4 in 22% and 30% of all patients, respectively. Other hematological and non-hematological toxicities were moderate and did not exceed grade 2. Nineteen patients had measurable disease and were available for efficacy. The overall response rate was 73.7% (95% confidence interval: 53.5-93.9%) for patients with measurable disease including 3 patients with complete remission. Conclusions: The recommended dose of cisplatin in combination with the IF-regimen is 50 mg/ m2 every two weeks. The triple combination of irinotecan, (leucovorin) infusional 5-fluorouracil and cisplatin appears to have therapeutic efficacy with manageable toxicity in advanced gastric cancer.
    TumorDiagnostik &amp Therapie 12/2006; 27(6):255-258. DOI:10.1055/s-2006-927158
  • S. Schietzel · F. Burrows · J. Fletcher · S. Seeber · S. Bauer ·

    EJC Supplements 11/2006; 4(12):168-168. DOI:10.1016/S1359-6349(06)70560-2 · 9.39 Impact Factor

Publication Stats

5k Citations
1,185.62 Total Impact Points


  • 2008-2009
    • Universitätsklinikum Erlangen
      Erlangen, Bavaria, Germany
    • Kliniken Essen-Mitte Knappschafts-Krankenhaus
      Essen, North Rhine-Westphalia, Germany
  • 1982-2008
    • University of Duisburg-Essen
      • Department of Internal and Integrative Medicine
      Essen, North Rhine-Westphalia, Germany
  • 2001-2007
    • Friedrich-Alexander-University of Erlangen-Nürnberg
      • Biochemistry
      Erlangen, Bavaria, Germany
    • Roswell Park Cancer Institute
      • Grace Cancer Drug Center
      Buffalo, New York, United States
  • 2006
    • Kinki University
      Ōsaka, Ōsaka, Japan
  • 1974-2006
    • University Hospital Essen
      • • Institute of Immunology
      • • Klinik für Urologie
      • • Clinic for Internal Medicine (Tumor Research)
      Essen, North Rhine-Westphalia, Germany
  • 1986-2002
    • German Cancer Research Center
      Heidelburg, Baden-Württemberg, Germany
  • 1999
    • Universitätsklinikum des Saarlandes
      Homburg, Saarland, Germany
  • 1997-1998
    • LungenClinic Grosshansdorf
      Hamburg, Hamburg, Germany
  • 1996
    • Katholisches Klinikum Essen
      Essen, North Rhine-Westphalia, Germany
  • 1995
    • medac GmbH
      Wedel, Schleswig-Holstein, Germany
  • 1993
    • Friedrich Schiller University Jena
      • Clinic of Internal Medicine II
      Jena, Thuringia, Germany
  • 1992-1993
    • Hannover Medical School
      Hanover, Lower Saxony, Germany
  • 1988-1990
    • Städtisches Krankenhaus Kiel
      Kiel, Schleswig-Holstein, Germany
  • 1984
    • Universität Regensburg
      Ratisbon, Bavaria, Germany
  • 1981
    • University Medical Center Hamburg - Eppendorf
      Hamburg, Hamburg, Germany
  • 1972-1979
    • Baylor College of Medicine
      • • Department of Pharmacology
      • • Department of Pediatrics
      Houston, Texas, United States