Sheng-Sheng Li

Publications (2)0 Total impact

• Article: [Effects of high magneto-gravitational environment on calcium/calmodulin signal of MG63 osteoblast-like cells].

  Ming-zhi Luo, Sheng-sheng Li, Xiang Gao, Yi Lu, Zhou-qi Yang, Wei Zhang, Peng Shang
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  ABSTRACT: To investigate the effects of high magneto-gravitational environment on Ca(2+);]/calmodulin (CaM) signal of MG63 osteoblast-like cells. A special designed large gradient high magnetic field could produce three different high magneto-gravitational environments including μg (12 T), 1 g (16 T) and 2 g (12 T). The effects of high magneto-gravitational environments on intracellular free Ca(2+);] concentration ([Ca(2+);](i);) and protein expression including calmodulin (CaM), myosin light chain kinases (MLCK) and phosphorylated Ca(2+);]/CaM dependent protein kinase II(pCaMKII) were measured by Fluo-3/AM or Western blot, respectively. When compared with control group, an increase of [Ca(2+);](i); of MG63 was caused by strong magnetic field; Compared to 2 g, μg decreased [Ca(2+);](i); of MG63. The protein expression of CaM and pCaMKIIof MG63 cells was decreased by simulated weightlessness. [Ca(2+);](i); of MG63 cells was increased by strong magnetic field; simulated weightlessness inhibited Ca(2+);/CaM signaling of MG63 cells.
  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 03/2012; 28(3):225-7.
• Article: [Roles of autocrine soluble fibronectin on the maintenance of cell shape of osteosarcoma MG-63 cells].

  Ming-Zhi Luo, Jing-Bao Li, Sheng-Sheng Li, Jian-Ping Cao, Rui Meng, Wei Zhang, Peng Shang
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  ABSTRACT: To investigate the roles of autocrine soluble FN of MG-63 cells in cell shape maintaining. Human osteosarcoma MG-63 cells (1 × 10(4); cells/cm(2);) were routinely cultured in MEM medium+10% fetal bovine serum (FBS) for 24 h and then the medium was replaced by fresh medium(the group without autocrine soluble FN), conditional medium of collected after 24 h of MG-63 cells (the group containing autocrine soluble FN ) and fresh medium with 20 microg/mL FN( the group with plasma FN) after 24 h, and the cell morphology was observed using phase contrast microscope; The concentrations of soluble FN in fresh and conditional medium were detected; Microfilament and insoluble FN changes of MG-63 cells were investigated. The concentration of soluble FN in conditional medium is much higher than that in the fresh medium (P<0.01). Fresh medium will result in the cell shape changing from spindle to round for 0.5 h. The cell began to spread for 1 h, and the cell shape recovered for 2 h; Conditioned medium has no significant effects on cell shape. Addition of FN (20 mg/L) to fresh medium could inhibit the cell shape change induced by fresh medium. Microfilament and insoluble FN were disorganized by fresh medium. These results indicated that autocrine soluble FN of osteosarcoma MG-63 cells may be involved in cell shape maintaining.
  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 12/2010; 26(12):1210-3.