S Dekio

Shimane University, Matsu, Shimane Prefecture, Japan

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Publications (71)121.44 Total impact

  • Schizophrenia Research 02/2000; 41(2):383-6. · 4.59 Impact Factor
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    ABSTRACT: To investigate whether mast cells (MCs) and chymase, the major protease of murine MCs, were involved in a chronic fibroproliferative disorder of the paws associated with type II collagen (CII)-induced arthritis. Eighteen DBA/1J mice were divided into 3 groups and were used to study fibroproliferative changes in paws elicited by immunization. Arthritis was induced by immunization with CII, which was intradermally injected as an emulsion made with adjuvant. A booster shot was done 3 weeks after the initial shot. A group with no treatment and that received adjuvant alone served as control. Twelve weeks after the booster shot, inflammation of the paws was evaluated for pathological and biochemical indices. Chymase activity was determined with a chromogenic peptide substrate. In CII-immunized group, collagen bundles accumulated around the destructed joints. In accordance with the pathological findings, MC density in the affected paws was increased (154.8+/-13.3/mm2; p<0.05 vs. control) and chymase activity was also increased (29.5+/-2.8 mU/mg protein; p<0.01 vs. control). The present results demonstrate increases in MCs and chymase in fibroproliferative paws of collagen-induced arthritic mice.
    Inflammation Research 07/1999; 48(6):318-24. · 1.96 Impact Factor
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    ABSTRACT: A 72-year-old Japanese man with eccrine poroma on the arm is described. To the best of our knowledge, he is the fifth patient with this tumor on the arm reported from Japan. The histopathological type of the tumor of our patient was unique because it was acanthotic; those of the previous patients were all of the intradermal type.
    The Journal of Dermatology 02/1999; 26(1):67-9. · 1.77 Impact Factor
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    ABSTRACT: In the present study, we have found that the cell lysate from cultured human normal keratinocytes from foreskin (HFKs) hydrolyzed alpha-N-benzoyl-DL-arginine beta-naphthylamide (BANA), and the BANA hydrolysis occurred most under conditions of 37 degrees C and pH 6.0. This activity was strongly inhibited by leupeptin, which is an inhibitor to cathepsin B. These results suggested that the cell lysate from cultured HFKs contained cathepsin B-like enzyme activity. This is the first report to demonstrate that cathepsin B-like enzyme activity was expressed in the cell lysate from human normal keratinocytes.
    Human Cell 01/1999; 11(4):239-42. · 1.41 Impact Factor
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    ABSTRACT: Inflammation, granulation, and collagen accumulation, which are observed in the wound healing process, occasionally lead to hypertrophic scarring. Several in vitro reports have suggested that skin mast cells (MCs) and their major protease, chymase, participate in the healing process as well as in fibrotic skin diseases. The present study examined the potential involvement of MCs and MC chymase in the healing of burns in mouse dorsal skin. The size of the burn wounds, density of the capillaries, collagen accumulation, MC number, and chymase activity were measured before and 1, 3, 7, and 14 days after burning. The healing process corresponded strongly with MC density and chymase activity in both acute and subacute phases. The maximum decrease in MC number and chymase activity occurred on day 3 when tissue loss due to necrosis was maximal. From day 7 to 14, the burn wounds retracted rapidly accompanied by increases in capillaries and collagen fibers, in correspondence with fast increments in MC numbers and chymase activity at the wound edges. The present results combined with previous in vitro results strongly support the contention that skin MC chymase plays a role in the normal wound healing process, and presumably in dermal fibrotic disorders.
    Archives for Dermatological Research 11/1998; 290(10):553-60. · 2.71 Impact Factor
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    ABSTRACT: The effect of lead acetate (Pb) on the formation of capillary-like structures (tube formation) by cultured human umbilical vascular endothelial cells (HUVECs) was examined. HUVECs were seeded on a gelled basement membrane matrix (Matrigel). Treatment of HUVECs with 0.3-30.0 microM Pb for 24 hours inhibited the tube formation dose-dependently. The length of tube formation decreased time-dependently with 3.0-10.0 microM Pb. To elucidate the main target factor of Pb for this inhibition, the effects of Pb on the activity of protein kinase C (PKC) and Matrigel were examined. The addition of beta-phorbol 12-myristate 13-acetate (PMA, 50 nM), an activator of PKC, and isoquinolinesulfonamide derivative (H-7, 30 microM), an inhibitor of PKC, showed an increase and decrease in the tube formation, respectively. However, the results of simultaneous addition of Pb and either PMA or H-7 to HUVECs indicated that PMA and H-7 acted not synergistically but additively. When PKC activities in HUVECs were measured by a colorimetric assay after treatments with 3.0-10.0 microM Pb for 24 hours, there was no significant change in PKC activity in the cells. The Pb-inhibition of tube formation was suggested to be independent of PKC activity. Pretreatment of Matrigel with 3.0-10.0 microM Pb for different periods decreased the tube formation dose- and time-dependently. These findings suggest that Pb can inhibit the tube formation by HUVECs dose- and time-dependently and that the inhibitory effect of Pb could be dependent on the degeneration of Matrigel, not on PKC activity.
    Human Cell 12/1997; 10(4):283-91. · 1.41 Impact Factor
  • The Journal of Dermatology 11/1997; 24(10):682-3. · 1.77 Impact Factor
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    ABSTRACT: We report a 68-year-old Japanese woman with angiosarcoma (AS) on the abdominal wall. To the best of our knowledge, she is the third such patient reported from Japan.
    The Journal of Dermatology 06/1997; 24(5):342-4. · 1.77 Impact Factor
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    ABSTRACT: A 39-year-old Japanese woman suffering from plaque stage of mycosis fungoides (MF) developed Kaposi's varicelliform eruption (KVE) on her face. KVE appeared 1 month after commencing skin electron beam irradiation (SEBI), when the total irradiation had reached 46 Gy. Natural killer (NK) cell activity in the peripheral blood was abnormally low, but returned to normal after 1 year. However, lymphocyte reactivity to mitogens was persistently low. These facts suggested that the KVE in our patient developed because of abnormally low NK cell reactivity probably induced by radiation therapy.
    Clinical and Experimental Dermatology 02/1997; 22(1):41-3. · 1.33 Impact Factor
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    ABSTRACT: In a male Japanese patient, prurigo nodularis (PN) appeared in association with gastric cancer. The cutaneous pruriginous lesions dramatically improved soon after total gastrectomy without any treatment for the skin lesions. Peripheral eosinophilia seen before the operation also rapidly disappeared. These data suggest that some cytokines involved in gastric cancer might have played an important role in the development of PN in our patient.
    The Journal of Dermatology 11/1996; 23(10):703-7. · 1.77 Impact Factor
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    ABSTRACT: The monoclonal nonspecific suppressor factor (MNSF), a lymphokine produced by a murine T cell hybridoma, shows a pleiotropic antigen-nonspecific suppressive function. Most recently, a cDNA encoding a subunit of MNSF (MNSF beta) has been isolated and characterized. Recombinant form of MNSF beta (rMNSF beta) inhibits lymphokine functions, as does native MNSF. In this study, we investigated whether rMNSF beta also affects macrophage function in terms of LPS-induced TNF-alpha production by a mouse macrophage cell line, J774. rMNSF beta suppressed the TNF-alpha production in a dose-dependent manner. This suppressive effect was remarkably reduced when rMNSF beta was added after 6 h of LPS stimulation. In addition, enhancement of TNF-alpha production by IFN-gamma was also suppressed by rMNSF beta. The suppressive effect was partly neutralized by the addition of the serine/threonine phosphatase inhibitor, okadaic acid. This finding suggests that serine/threonine protein phosphatases type 1 and/or 2A may be implicated in the mechanism of action of MNSF.
    Immunobiology 08/1996; 195(2):187-98. · 2.81 Impact Factor
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    ABSTRACT: A tick which bit the glans penis of a 40-year-old Japanese man was surgically excised together with the skin where the bite occurred. It was identified as Amblyomma testudinarium through the taxonomical investigation of its morphological characteristics. To our best knowledge, our patient is the sixth tick bite from this species of tick recorded in the Chugoku District of Japan.
    The Journal of Dermatology 03/1996; 23(2):136-8. · 1.77 Impact Factor
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    ABSTRACT: The monoclonal nonspecific suppressor factor (MNSF), a lymphokine produced by a murine T cell hybridoma, shows a pleiotropic antigen-nonspecific suppressive function. Most recently, a cDNA encoding a subunit of MNSF (MNSFβ) has been isolated and characterized. Recombinant form of MNSFβ (rMNSFβ) inhibits lymphokine functions, as does native MNSF. In this study, we investigated whether rMNSFβ also affects macrophage function in terms of LPS-induced TNF-α production by a mouse macrophage cell line, J774. rMNSFβ suppressed the TNF-α production in a dose-dependent manner. This suppressive effect was remarkably reduced when rMNSFβ was added after 6 h of LPS stimulation. In addition, enhancement of TNF-α production by IFN-γ was also suppressed by rMNSFβ. The suppressive effect was partly neutralized by the addition of the serine/threonine phosphatase inhibitor, okadaic acid. This finding suggests that serine/threonine protein phosphatases type 1 and/or 2A may be implicated in the mechanism of action of MNSF.
    Immunobiology 01/1996; 195(2):187-198. · 2.81 Impact Factor
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    ABSTRACT: Clarithromycin (CAM) was assessed for in vitro antimicrobial activity against various mycobacterial species. Except for Mycobacterium tuberculosis, CAM displayed MICs for test mycobacteria comparable to those of sparfloxacin (SPFX) but lower than those of rifampicin (RMP) and ofloxacin (OFLX). CAM at 0.25 mug/ml (1 MIC) exhibited bactericidal action against M. intracellulare growing in 7H9 medium and at 1 or 10 mug/ml (4 and 40 MIC, respectively) CAM displayed antimicrobial activity against the organism phagocytosed in murine macrophages, while RMP failed to manifest such an effect. When CAM was given s.c. or by gavage to mice infected i.v. with M. intracellulare at doses of 0.2 to 2 mg/mouse once daily, six times per week, it exhibited significant therapeutic efficacy in terms of decreased incidence of gross lung lesions and reduced bacterial loads in the lungs and spleen.
    International Journal of Antimicrobial Agents 09/1994; 4(3):175-81. · 4.42 Impact Factor
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    ABSTRACT: A 79-year-old Japanese man with a trichilemmal carcinoma (TC) on the right thigh is described. To the best of our knowledge, he is the fifth patient with a TC on the thigh reported from Japan.
    The Journal of Dermatology 08/1994; 21(7):494-6. · 1.77 Impact Factor
  • S Dekio, J Jidoi
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    ABSTRACT: A 70-year-old Japanese housewife, a resident of Shimane Prefecture, developed an erythematous, annular skin lesion on her right forearm. Mycological examinations revealed that it was tinea corporis caused by Trichophyton verrucosum (T. verrucosum). This fungus was then shown to have been transmitted to her from young dairy cattle introduced from the Hokkaido District to the ranch of her son. To the best of our knowledge, she is the first reported patient with tinea due to T. verrucosum who was infected in the San'in District.
    The Journal of Dermatology 06/1994; 21(5):347-51. · 1.77 Impact Factor
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    ABSTRACT: S-carboxymethylated (SCM) fibrous proteins (FPs) from the scalp hairs of the three different hereditary hair abnormalities (trichorrhexis invaginata in Netherton's syndrome, pili trianguli et canaliculi in uncombable hair syndrome, and fine hair in anhidrotic ectodermal dysplasia) were analyzed by two-dimensional polyacrylamide gel electrophoresis. Comparison of the SCM FP compositions of the three hair abnormalities with that of the normal revealed that the SCM FP compositions of these three abnormal hairs were all electrophoretically different from each other and that of the normal.
    The Journal of Dermatology 06/1994; 21(5):312-8. · 1.77 Impact Factor
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    ABSTRACT: We describe here a 35-year-old Japanese woman with mycosis fungoides (MF) who developed numerous, rapidly-growing, seborrheic keratoses on the front of her neck and chest. To the best of our knowledge, this is the first reported case of MF with Leser-Trélat sign from Japan.
    The Journal of Dermatology 04/1994; 21(3):189-93. · 1.77 Impact Factor
  • S Dekio, J Jidoi, Y Yamada
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    ABSTRACT: A 77-year-old Japanese woman, a resident of Shimane Prefecture, with black dot ringworm caused by a Trichophyton glabrum infection on the scalp is described. To the best of our knowledge, she is the first case of BDR reported from the San'in District.
    The Journal of Dermatology 03/1994; 21(2):128-32. · 1.77 Impact Factor
  • S Dekio, J Jidoi
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    ABSTRACT: A 52-year-old Japanese female patient with ringworm-like cutaneous candidiasis on the right axillary area is described. It appeared to have developed in relationship to previous local steroid therapy in this area for a long time.
    The Journal of Dermatology 01/1994; 20(12):783-5. · 1.77 Impact Factor