Moaine Elbaidouri

Publications (2)10.78 Total impact

• Article: Comparative Genomic Paleontology Across Plant Kingdom Reveals The Dynamics Of TE-driven Genome Evolution.

  Moaine Elbaidouri, Olivier Panaud
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  ABSTRACT: Long terminal repeat-retrotransposons (LTR-RTs) are the most abundant class of transposable elements in plants. They strongly impact the structure, function and evolution of their host genome and, in particular, their role in genome size variation has been clearly established. However, the dynamics of the process through which LTR-RTs have differentially shaped plant genomes is still poorly understood because of a lack of comparative studies. Using a new robust and automated family classification procedure, we exhaustively characterized the LTR-RTs in 8 plant genomes for which a high quality sequence is available (i.e. Arabidopsis thaliana, A. lyrata, grapevine, soybean, rice, Brachypodium dystachion, sorghum and maize). This allowed us to perform a comparative genome-wide study of the retrotranspositional landscape in these 8 plant lineages from both monocots and dicots. We show that retrotransposition has recurrently occurred in all plant genomes investigated, regardless their size and through bursts, rather than a continuous process. Moreover, in each genome, only one or few LTR-RT families have been active in a recent past and the difference in genome size among the species studied could thus mostly be accounted for by the extent of the latest transpositional burst(s). Following these bursts, LTR-RTs are efficiently eliminated from their host genomes through recombination and deletion, but we show that the removal rate is not lineage-specific. These new findings leads us to propose a new model of TE-driven genome evolution in plants.
  Genome Biology and Evolution 02/2013; · 4.62 Impact Factor
• Article: Transpositional landscape of the rice genome revealed by paired-end mapping of high-throughput re-sequencing data.

  François Sabot, Nathalie Picault, Moaine El-Baidouri, Christel Llauro, Cristian Chaparro, Benoit Piegu, Anne Roulin, Emmanuel Guiderdoni, Mélissa Delabastide, Richard McCombie, Olivier Panaud
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  ABSTRACT: Transposable elements (TEs) are mobile entities that densely populate most eukaryotic genomes and contribute to both their structural and functional dynamics. However, most TE-related sequences in both plant and animal genomes correspond to inactive, degenerated elements, due to the combined effect of silencing pathways and elimination through deletions. One of the major difficulties in fully characterizing the molecular basis of genetic diversity of a given species lies in establishing its genome-wide transpositional activity. Here, we provide an extensive survey of the transpositional landscape of a plant genome using a deep sequencing strategy. This was achieved through paired-end mapping of a fourfold coverage of the genome of rice mutant line derived from an in vitro callus culture using Illumina technology. Our study shows that at least 13 TE families are active in this genotype, causing 34 new insertions. This next-generation sequencing-based strategy provides new opportunities to quantify the impact of TEs on the genome dynamics of the species.
  The Plant Journal 01/2011; 66(2):241-6. · 6.16 Impact Factor