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ABSTRACT: Abstract Groin lymph node dissection (GLND) remains an effective treatment for malignant neoplasms of the skin arising on the lower extremities and perineum. However, complications such as seroma, flap necrosis, and infections have been encountered. It is thought that a conventional operation using a long inguinal incision can result in those complications. To minimise the risk of such complications, endoscopic GLND (EGLND) was performed in five patients. No severe complications were found. Although EGLND is still in the developmental stage, this technique is suggested as a possible surgical option for reducing morbidity and improving aesthetic results.
Journal of plastic surgery and hand surgery. 04/2013;
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The American Journal of dermatopathology 03/2013; · 1.30 Impact Factor
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Acta Dermato-Venereologica 08/2012;
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ABSTRACT: Fatty acid binding proteins (FABPs) are capable of binding long-chain FA and are involved in intracellular FA transport and signal transduction. In sebaceous glands, FABP5 is highly expressed in differentiated sebocytes; though, its function remains unclear. In this study, we examined the role of FABP5 in sebocytes using FABP5-deficient mice. The size of sebaceous glands was significantly reduced, while the sebum volume was increased with altered lipid composition in FABP5-deficient mice. However, no significant differences were discerned in the expression of proliferation or differentiation markers including Blimp1, c-myc, Ki67 and peroxisome proliferator-activated receptors (PPAR)γ between wild-type and FABP5-deficient sebaceous glands. The expression of cellular retinoic acid binding protein-2 (CRABP2) that is a competitor of FABP5 for RA signalling was increased in FABP5-deficient mice. These results suggest that FABP5 is involved in the regulation of sebaceous gland activity through modulation of cellular lipid signalling and/or metabolism in the sebocytes.
Experimental Dermatology 07/2012; 21(7):543-6. · 3.54 Impact Factor
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The Journal of Dermatology 01/2012; · 1.49 Impact Factor
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Acta Dermato-Venereologica 06/2011; 91(5):589-90.
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ABSTRACT: It has not been confirmed whether tight junctions (TJs) function as a paracellular permeability barrier in adult human skin. To clarify this issue, we performed a TJ permeability assay using human skin obtained from abdominal plastic surgery. Occludin, a marker protein of TJs, was expressed in the granular layer, in which a subcutaneously injected paracellular tracer, Sulfo-NHS-LC-Biotin (556.59 Da), was halted. Incubation with ochratoxin A decreased the expression of claudin-4, an integral membrane protein of TJs, and the diffusion of paracellular tracer was no longer prevented at the TJs. These results demonstrate that human epidermis possesses TJs that function as an intercellular permeability barrier at least against small molecules (∼550 Da). UVB irradiation of human skin xenografts and human skin equivalents (HSEs) resulted in functional deterioration of TJs. Immunocytochemical staining of cultured keratinocytes showed that occludin was localized into dot-like shapes and formed a discontinuous network when exposed to UVB irradiation. Furthermore, UVB irradiation downregulated the active forms of Rac1 and atypical protein kinase C, suggesting that their inactivation caused functional deterioration of TJs. In conclusion, TJs function as a paracellular barrier against small molecules (∼550 Da) in human epidermis and are functionally deteriorated by UVB irradiation.
Journal of Investigative Dermatology 03/2011; 131(3):744-52. · 6.31 Impact Factor
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The Journal of Dermatology 12/2010; 37(12):1060-2. · 1.49 Impact Factor
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Journal of dermatological science 11/2010; 62(1):70-1. · 3.71 Impact Factor
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Eisaku Ogawa,
Yuji Owada,
Shuntaro Ikawa,
Yasuhiro Adachi,
Teie Egawa,
Kei Nemoto,
Kaori Suzuki,
Takanori Hishinuma,
Hiroshi Kawashima,
Hisatake Kondo, Masahiko Muto,
Setsuya Aiba,
Ryuhei Okuyama
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ABSTRACT: Fatty acid-binding proteins (FABPs) are postulated to serve as lipid shuttles that solubilize hydrophobic fatty acids and deliver them to appropriate intracellular sites. Epidermal FABP (E-FABP/FABP5) is predominantly expressed in keratinocytes and is overexpressed in the actively proliferating tissue characteristic of psoriasis and wound healing. In this study, we found decreased expression of the differentiation-specific proteins keratin 1, involucrin, and loricrin in E-FABP(-/-) keratinocytes relative to E-FABP(+/+) keratinocytes. We also determined that incorporation of linoleic acid was significantly reduced in E-FABP(-/-) keratinocytes. Although linoleic acid did not directly affect keratinocyte differentiation, keratin 1 expression was induced by the linoleic acid derivative 13(S)-hydroxyoctadecadienoic acid (13(S)-HODE), and this induction was concomitant with increased NF-κB activity. In E-FABP(-/-) keratinocytes, the expression of 13(S)-HODE and the subsequent induction of NF-κB activity was lower than in wild-type keratinocytes. The reduction of linoleic acid in E-FABP(-/-) keratinocytes led to decreased cellular 13(S)-HODE content, resulting in decreased keratin 1 expression through downregulation of NF-κB activity. The regulation of fatty acid metabolism by E-FABP during keratinocyte differentiation suggests that E-FABP may have a role in the pathogenesis of psoriasis.
Journal of Investigative Dermatology 11/2010; 131(3):604-12. · 6.31 Impact Factor
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ABSTRACT: Heat shock transcription factor 1 (HSF1) is a major transactivator of genes coding for heat shock proteins (Hsps). Recent studies demonstrate that HSF1 is involved in tumor initiation, maintenance, and progression by regulating the expression of Hsps and other molecular targets. However, the role of HSF1 in melanoma is largely unknown.
The aim of this study is to analyze the role of HSF1 in melanoma.
We used short hairpin RNA (shRNA) to silence HSF1 in the human melanoma MeWo cell line and investigated its effect on cell proliferation and in the efficacy of chemotherapy, hyperthermia and thermochemotherapy.
We found that proliferation of MeWo cells treated with HSF1 shRNA was markedly reduced, and this was associated with a G₁ block of the cell cycle, whereas HSF1 shRNA treatment of normal human keratinocyte HaCat cells had no effect on proliferation. Suppression of cell proliferation by silencing HSF1 was also observed in other melanoma cell lines, HMV-I and HMV-II. Furthermore, silencing HSF1 enhanced the sensitivity of MeWo cells to heat shock, yet did not affect their sensitivity to treatment with dacarbazine.
These findings show that HSF1 is required for normal growth and survival of melanoma cells under heat shock conditions, and suggests that HSF1 is a promising therapeutic target in melanoma.
Journal of dermatological science 10/2010; 60(3):187-92. · 3.71 Impact Factor
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The Journal of Dermatology 04/2010; 37(4):387-9. · 1.49 Impact Factor
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The Journal of Dermatology 04/2008; 35(3):178-80. · 1.49 Impact Factor
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ABSTRACT: We report a case of disto-lateral subungal onychomycosis of the first toe of the right foot of a 33-year-old female. The causative agent was identified as Aspergillus sydowii based on DNA sequencing and the macroscopic and microscopic characteristics of the culture. Initially, the medial one-third of the right first toenail was streaked with numerous yellow longitudinal stripes that had fused, with no symptoms of tinea pedis. Treatment with oral terbinafine and itraconazole was ineffective. Based on this case and on three other cases in the literature, the clinical characteristics of onychomycosis caused by A. sydowii can be summarized as follows: (i) no clinical history of symptoms of tinea pedis; (ii) lack of clinical response to oral antifungal agents; (iii) streaked and turbid appearance of the diseased nail and (iv) presence of dichotomous irregular hyphae, as detected by direct microscopic examination.
Mycoses 04/2008; 51(2):170-3. · 2.25 Impact Factor
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The Journal of Dermatology 02/2008; 35(1):36-8. · 1.49 Impact Factor
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The Journal of Dermatology 02/2008; 35(1):44-5. · 1.49 Impact Factor
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ABSTRACT: We describe a case of purpura fulminans due to septicemia after artificial abortion. Our patient suffered purpuric progressive skin necrosis on the back, extremities and buttock. Rhabdomyolysis involvement was confirmed by high level of creatinine phosphokinase and appearance of much brownish discharge from necrotic gluteal muscle and latissimus dorsi muscle. Amputation of both feet and second, third, fourth and fifth fingers of the right hand was performed. The buttock lesion was reconstructed with the posterolateral thigh V-Y flap after debridement. Other lesions were covered with split-thickness skin grafts.
The Journal of Dermatology 12/2007; 34(11):786-9. · 1.49 Impact Factor
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ABSTRACT: Monoclonal antibodies directed against type 12 Group A streptococcal cell wall antigens cross-react with nuclei and cytoplasm of cells from skin and synovium from controls, uninvolved skin of psoriatics and psoriatic plaques. Patients with psoriasis had high serum titres of antibody against the M12 (C-region) streptococcal antigen compared to controls. An abnormal immune response directed against a ‘self’ antigen after initiation by Group A streptococcal infection may play an important role in the exacerbation or development of psoriasis.
Australasian Journal of Dermatology 06/2007; 37(s1):S54 - S55. · 1.00 Impact Factor
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ABSTRACT: Human leukocyte antigen (HLA) frequencies in 67 melanoma patients were compared to 120 controls in Japan. The frequency of HLA-B13 was increased in melanoma patients compared to controls and was particularly common in nodular melanoma. HLA-B61 was decreased in melanoma patients. Almost 50% of acral lentiginous melanoma patients expressed HLA-B51 compared to 0% of nodular melanomas.
Australasian Journal of Dermatology 06/2007; 37(s1):S39 - S39. · 1.00 Impact Factor
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The Journal of Dermatology 04/2006; 33(3):230-1. · 1.49 Impact Factor
