Marcin Szaumkessel

Publications (8)16.16 Total impact

• Article: Heterogeneity of 11q13 region rearrangements in laryngeal squamous cell carcinoma analyzed by microarray platforms and fluorescence in situ hybridization.

  Małgorzata Jarmuz-Szymczak, Kinga Pelinska, Magdalena Kostrzewska-Poczekaj, Ewa Bembnista, Maciej Giefing, Damian Brauze, Marcin Szaumkessel, Andrzej Marszalek, Joanna Janiszewska, Katarzyna Kiwerska, Anna Bartochowska, Reidar Grenman, Witold Szyfter, Krzysztof Szyfter
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  ABSTRACT: We reinvestigated rearrangements occurring in region q13 of chromosome 11 aiming to: (i) describe heterogeneity of the observed structural alterations, (ii) estimate amplicon size and (iii) identify of oncogenes involved in laryngeal cancer progression as potential targets for therapy. The study included 17 cell lines derived from laryngeal cancers and 34 specimens from primary laryngeal tumors. The region 11q13 was analyzed by fluorescence in situ hybridization (FISH), array comparative genomic hybridization (aCGH) and gene expression microarray. Next, quantitative real time PCR was used for chosen genes to confirm results from aCGH and gene expression microarray. The observed pattern of aberrations allows to distinguish three ways, in which gain and amplification involving 11q13 region may occur: formation of a homogeneously staining region; breakpoints in/near 11q13, which lead to the three to sevenfold increase of the copy number of 11q13 region; the presence of additional copies of the whole chromosome 11. The minimal altered region of gain and/or amplification was limited to ~1.8 Mb (chr.11:69,395,184-71,209,568) and comprised mostly 11q13.3 band which contain 12 genes. Five, out of these genes (CCND1, ORAOV1, FADD, PPFIA1, CTTN) had higher expression levels in comparison to healthy controls. Apart from CCND1 gene, which has an established role in pathogenesis of head and neck cancers, CTTN, ORAOV1 and FADD genes appear to be oncogene-candidates in laryngeal cancers, while a function of PPFIA1 requires further studies.
  Molecular Biology Reports 05/2013; · 2.93 Impact Factor
• Article: Erratum to: Simple technique for RNA purification from mouse inner ear hair cells.

  Marcin Szaumkessel, Damian Brauze, Małgorzata Rydzanicz, Michał Karlik, Krzysztof Szyfter, Witold Szyfter, Maciej Wróbel
  Molecular Biology Reports 04/2012; 39(4):5047. · 2.93 Impact Factor
• Source

  Available from: InTech

  Chapter: DNA Repair Capacity and the Risk of Head and Neck Cancer

  Marcin Szaumkessel, Wojciech Gaw?cki, Krzysztof Szyfter
  03/2012; , ISBN: 978-953-51-0236-6
• Article: Simple technique for RNA purification from mouse inner ear hair cells.

  Marcin Szaumkessel, Damian Brauze, Małgorzata Rydzanicz, Michał Karlik, Krzysztof Szyfter, Witold Szyfter, Wróbel Maciej
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  ABSTRACT: Obtaining a good quality of RNA from small population of cells remain an issue. Isolation for a special anatomic location such as inner ear placed in the temporal bone become a challenge, especially in terms of time needed for isolation of living tissue from the bone, which is a key factor to preserve the RNA. Due to limited accessibility to the technologies such as laser dissection, we present a simplified procedure for isolation of good quality of RNA from the inner ear for further studies.
  Molecular Biology Reports 02/2012; 39(6):6467-9. · 2.93 Impact Factor
• Source

  Available from: Sylvia Hartmann

  Article: Loss of protein expression and recurrent DNA hypermethylation of the GNG7 gene in squamous cell carcinoma of the head and neck.

  Sylvia Hartmann, Marcin Szaumkessel, Itziar Salaverria, Ronald Simon, Guido Sauter, Katarzyna Kiwerska, Wojciech Gawecki, Magdalena Bodnar, Andrzej Marszalek, Julia Richter, Damian Brauze, Natalia Zemke, Malgorzata Jarmuz, Martin-Leo Hansmann, Reiner Siebert, Krzysztof Szyfter, Maciej Giefing
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  ABSTRACT: Although down-regulation of GNG7 in cancer was reported before, its role in carcinogenesis is poorly understood. It belongs to a family of large G-proteins that may be involved in cell-contact-induced growth arrest and function in tumor suppression. In the present study, we stained immunohistochemically 188 tumors derived from larynx or floor of the mouth for GNG7 protein and confronted it with clinicopathologic data. Moreover, we performed bisulfite pyrosequencing to analyze GNG7 promoter methylation. We identified recurrent loss of GNG7 protein expression in 68/188 (36%) cases and promoter hypermethylation in (42/98; 43%) primary tumors, predominantly in young patients (p < 0.001). Loss of GNG7 expression correlated with hypermethylation of GNG7 promoter region (p < 0.001). Moreover, loss of GNG7 protein expression correlated with tumor size (p = 0.012) and lack of cervical metastasis (p = 0.02) whereas sustained expression correlated with keratinization (p = 0.008). Taken together, loss of GNG7 protein expression is a frequent event in head and neck cancer. Moreover, our data suggest that hypermethylation of the promoter region of GNG7 is probably the mechanism of the observed inactivation.
  Journal of applied genetics 12/2011; 53(2):167-74. · 1.66 Impact Factor
• Article: Pyrosequencing-based DNA methylation profiling of Fanconi anemia/BRCA pathway genes in laryngeal squamous cell carcinoma.

  Marcin Szaumkessel, Julia Richter, Maciej Giefing, Malgorzata Jarmuz, Katarzyna Kiwerska, Holger Tönnies, Reidar Grenman, Simone Heidemann, Krzysztof Szyfter, Reiner Siebert
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  ABSTRACT: Fanconi anemia (FA) associated genes [FANCA, -B, -C, FANCD1(BRCA2), -D2, -E, -F, -G, -I, -L, -M, FANCN (PALB2), FANCJ(BRIP1) and FA-linked BRCA1] encode proteins of DNA damage response pathways mutated in FA patients. FA is characterized by congenital malformations, chromosomal instability and high cancer susceptibility. FA patients have a 500-700 times higher risk of head and neck squamous cell carcinoma (HNSCC) compared to the non-FA population. As DNA methylation comprises one of the known gene inactivation mechanisms in cancer we have investigated the methylation status of 13 FA and one FA-linked gene in order to assess the role of FA in sporadic laryngeal squamous cell carcinoma (LSCC) tumor samples. Thirteen laryngeal squamous carcinoma cell lines (UT-SCC) and 64 primary laryngeal carcinoma cases were analyzed by bisulfite pyrosequencing. DNA from buccal swabs of 10 healthy volunteers was used as a control group. Promoter regions of FANCA, BRCA1 and BRCA2 displayed recurrent alterations in the methylation levels in cancer samples as compared to buccal swabs controls. For FANCA, hypomethylation was observed in 11/13 cell lines (p<0.0003) and all 64 primary larynx samples (p<0.001) compared to buccal swabs. For BRCA1, 4/13 cell lines (p=0.04) and 3/58 primary laryngeal cases (p=0.22) showed hypomethylation. In BRCA2, all 13 cell lines (p<0.0001) 4/63 primary LSCC (p<0.01) showed hypermethylation as compared to controls. In conclusion, we show recurrent alterations of DNA methylation levels in three Fanconi anemia genes which might contribute to the pathogenesis of LSCC.
  International Journal of Oncology 08/2011; 39(2):505-14. · 2.40 Impact Factor
• Article: High resolution ArrayCGH and expression profiling identifies PTPRD and PCDH17/PCH68 as tumor suppressor gene candidates in laryngeal squamous cell carcinoma.

  Maciej Giefing, Natalia Zemke, Damian Brauze, Magdalena Kostrzewska-Poczekaj, Magdalena Luczak, Marcin Szaumkessel, Kinga Pelinska, Katarzyna Kiwerska, Holger Tönnies, Reidar Grenman, Marek Figlerowicz, Reiner Siebert, Krzysztof Szyfter, Malgorzata Jarmuz
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  ABSTRACT: Many classical tumor suppressor genes (TSG) were identified by delineation of bi-allelic losses called homozygous deletions. To identify systematically homozygous deletions in laryngeal squamous cell carcinoma (LSCC) and to unravel novel putative tumor suppressor genes, we screened 10 LSCC cell lines using high resolution array comparative genomic hybridization (arrayCGH) and array based expression analysis. ArrayCGH identified altogether 113 regions harboring protein coding genes that showed strong reduction in copy number indicating a potential homozygous deletion. Out of the 113 candidate regions, 22 novel homozygous deletions that affected the coding sequences of 15 genes were confirmed by multiplexPCR. Three genes were homozygously lost in two cell lines: PCDH17/PCH68, PRR20, and PTPRD. For the 15 homozygously deleted genes, four showed statistically significant downregulation of expression in LSCC cell lines as compared with normal human laryngeal controls. These were ATG7 (1/10 cell line), ZMYND11 (BS69) (1/10 cell line), PCDH17/PCH68 (9/10 cell lines), and PTPRD (7/10 cell lines). Quantitative real-time PCR was used to confirm the downregulation of the candidate genes in 10 expression array-studied cell lines and an additional cohort of cell lines; statistical significant downregulation of PCDH17/PCH68 and PTPRD was observed. In line with this also Western blot analyses demonstrated a complete absence of the PCDH17 and PTPRD proteins. Thus, expression profiling confirmed recurrent alterations of two genes identified primarily by delineation of homozygous deletions. These were PCDH17/PCH68, the protocadherin gene, and the STAT3 inhibiting receptor protein tyrosine phosphatase gene PTPRD. These genes are good candidates for novel TSG in LSCC.
  Genes Chromosomes and Cancer 03/2011; 50(3):154-66. · 3.31 Impact Factor
• Article: [An attempt to rationalize adverse health effects following tobacco smoking].

  Krzysztof Szyfter, Joanna Janiszewska, Kinga Pelińska, Marcin Szaumkessel
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  ABSTRACT: Statistics concerning tobacco smoking confronted with cancer epidemiology indicates that only a minority of smokers develops terminal cancer. To much extent it could be explained by genetic polymorphism responsible for a variable risk to develop cancer and its further progression. Then, a comparative analysis of the data concerning cancer deaths regarding a decline of smoking in developed countries unravels other factors previously not considered to represent carcinogenic agents. Human papilloma virus (HPV) could serve as an example of such agent exerting adverse health effects once attributed only to tobacco.
  Przegla̧d lekarski 01/2010; 67(10):1052-5.