[Show abstract][Hide abstract] ABSTRACT: A molecular survey for pathogenic Leptospira spp. in kennelled dogs in Sicily, Italy, showed up to 16% of positive results. A fatal leptospirosis case was confirmed in a stray dog in the city of Palermo in May 2012. The dog, a mixed breed male, was recovered for therapy but died within an hour. Inflammation was present in the liver and kidneys. Haematoxylin-eosin staining of the kidney tissue revealed interstitial nephrite indicative of leptospirosis. The meninges were congested with some icteric shades. Several organs and prepuce swabs showed positive reaction by PCR specific for pathogenic Leptospira spp. The serum analysis by MAT (microscopic agglutination test) showed positive results for Leptospira serovar Canicola with a titre of 1:400. Additionally, five clinical cases of canine leptospirosis were suspected over a two years period (2011-2012) in the province of Palermo, in contrast to the six in a previous 5 years period. An increased concern of the veterinarians on leptospirosis in pet dogs should be suggested ., 2014. Canine leptospirosis cases and molecular screening for Leptospira interrogans infection. Pak Vet J, 34(2): 260-262.
[Show abstract][Hide abstract] ABSTRACT: Spirocerca lupi (Rudolphi 1809) is a cosmopolitan nematode of dogs and wild carnivores. In the past it has been reported in Italy, mainly in southern regions and in Sicily, where the parasite was observed in foxes in 2005. The parasite typically produces nodular masses in the oesophagus and thoracic aorta. During the 2003-2004 hunting season, the authors investigated a total of 55 foxes (Vulpes vulpes) hunted or killed by car accidents in the provinces of Palermo and Agrigento. All the foxes were subjected to necropsy and 6 (9.16%) had S. lupi nodules located exclusively in the gastric wall. The nature of the nodules was determined by opening them and detecting the nematodes inside, which were identified as S. lupi. Some of the nodules were characterized anatomopathologically and histopathologically. The formation of the parasitic nodule in the stomach only suggests a deviation from the route commonly followed by the nematode to reach the oesophagus, the elective anatomical site for completion of its lifecycle. This survey gives a contribution to the epidemiology of this parasite which is severely outdated in Italy and highlights some distinctive features of the life cycle and parasite migration.