[Show abstract][Hide abstract] ABSTRACT: This study assessed the physical, chemical, and microbiological characteristics of traditional Maiorchino cheese (Italy) made from raw ewe’s milk or from a mixture with goat’s milk. Cheese samples from the same batch were analyzed after 20 days and 6, 8, 12, 17 and 24 months of ripening. A decrease in moisture level lead to progressive total solids concentration (fat, total nitrogen, total solids and chloride) during ripening. Aw values decreased from 0.97 (day 20) to 0.85 (month 24), while pH increased from 4.99 to 5.41 (6 months) followed a by reduction until 4.85 (month 24). In samples analysed 20 days after cheesemaking, aerobic mesophilic count was 1.8•107 CFU/g, Enterobacteriaceae were 2.7•106 CFU/g, Staphylococcus spp. were 1.8•104 CFU/g, and yeasts 4.5•105 CFU/g. Sulphite reducing bacteria were not found. Lactic bacteria count at 30°C (LAB30) and 42°C (LAB42) was about 108 CFU/g (day 20); LAB30 reduced until month 8; LAB 42 reduced until month 12; both were not detectable at months 17 and 24. Cheese-making process does not consider commercial starter cultures and LAB group is heterogeneous because of its natural microflora. Yeasts were considered as typical microflora of Maiorchino. Volatile compounds were examined at 6, 12 and 24 months of ripening; 54 components were identified. Statistical analysis showed that the seasoning period of 12 months was the best for Maiorchino flavour attributes. The characterisation of Maiorchino traditional cheese may be considered as significant for this old traditional product, with the aim of obtaining the PDO certification.
Italian Journal of Food Safety 02/2015; 4(1). DOI:10.4081/ijfs.2015.4532
[Show abstract][Hide abstract] ABSTRACT: A molecular survey for pathogenic Leptospira spp. in kennelled dogs in Sicily, Italy, showed up to 16% of positive results. A fatal leptospirosis case was confirmed in a stray dog in the city of Palermo in May 2012. The dog, a mixed breed male, was recovered for therapy but died within an hour. Inflammation was present in the liver and kidneys. Haematoxylin-eosin staining of the kidney tissue revealed interstitial nephrite indicative of leptospirosis. The meninges were congested with some icteric shades. Several organs and prepuce swabs showed positive reaction by PCR specific for pathogenic Leptospira spp. The serum analysis by MAT (microscopic agglutination test) showed positive results for Leptospira serovar Canicola with a titre of 1:400. Additionally, five clinical cases of canine leptospirosis were suspected over a two years period (2011-2012) in the province of Palermo, in contrast to the six in a previous 5 years period. An increased concern of the veterinarians on leptospirosis in pet dogs should be suggested ., 2014. Canine leptospirosis cases and molecular screening for Leptospira interrogans infection. Pak Vet J, 34(2): 260-262.
[Show abstract][Hide abstract] ABSTRACT: Spirocerca lupi (Rudolphi 1809) is a cosmopolitan nematode of dogs and wild carnivores. In the past it has been reported in Italy, mainly in southern regions and in Sicily, where the parasite was observed in foxes in 2005. The parasite typically produces nodular masses in the oesophagus and thoracic aorta. During the 2003-2004 hunting season, the authors investigated a total of 55 foxes (Vulpes vulpes) hunted or killed by car accidents in the provinces of Palermo and Agrigento. All the foxes were subjected to necropsy and 6 (9.16%) had S. lupi nodules located exclusively in the gastric wall. The nature of the nodules was determined by opening them and detecting the nematodes inside, which were identified as S. lupi. Some of the nodules were characterized anatomopathologically and histopathologically. The formation of the parasitic nodule in the stomach only suggests a deviation from the route commonly followed by the nematode to reach the oesophagus, the elective anatomical site for completion of its lifecycle. This survey gives a contribution to the epidemiology of this parasite which is severely outdated in Italy and highlights some distinctive features of the life cycle and parasite migration.