Liao-sheng Zhou

Shandong Qianfoshan Hospital, Chi-nan-shih, Shandong Sheng, China

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Publications (1)2.35 Total impact

  • Source
    Wen-tong Fang, Hong-jian Li, Liao-sheng Zhou
    [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the protective effects of prostaglandin E(1) (PGE(1)) against H(2)O(2)-induced oxidative damage on human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated with PGE(1) (0.25, 0.50, and 1.00 micromol/L) for 24 h and exposed to H(2)O(2) (200 micromol/L) for 12 h, and cell viability was measured by the MTT assay. LDH, NO, SOD, GSH-Px, MDA, ROS, and apoptotic percentage were determined. eNOS expression was measured by Western blotting and real-time PCR. PGE(1) (0.25-1.00 micromol/L) was able to markedly restore the viability of HUVECs under oxidative stress, and scavenged intracellular reactive oxygen species induced by H(2)O(2). PGE(1) also suppressed the production of lipid peroxides, such as MDA, restored the activities of endogenous antioxidants including SOD and GSH-Px, and inhibited cell apoptosis. In addition, PGE(1) significantly increased NO content, eNOS protein, and mRNA expression. PGE(1) effectively protected endothelial cells against oxidative stress induced by H(2)O(2), an activity that might depend on the up-regulation of NO expression.
    Acta Pharmacologica Sinica 03/2010; 31(4):485-92. · 2.35 Impact Factor

Publication Stats

5 Citations
2.35 Total Impact Points

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Institutions

  • 2010
    • Shandong Qianfoshan Hospital
      Chi-nan-shih, Shandong Sheng, China
    • Shandong University
      • Department of Clinical Pharmacy
      Jinan, Shandong Sheng, China