Lidia Rutkowska-Sak

Publications of Lidia Rutkowska-Sak

• Etanercept treatment in juvenile idiopathic arthritis: the Polish registry.

  Authors: Zbigniew Zuber, Lidia Rutkowska-Sak, Jacek Postępski, Bogna Dobrzyniecka, Violetta Opoka-Winiarska, Katarzyna Kobusińska, Piotr Gietka, Violetta Osińska, Dorota Turowska-Heydel, Izabela Szczygielska, Beata Kołodziejczyk, Anna Świątek-Baczkowska, Agnieszka Gazda, Piotr Wiland, Witold Tłustochowicz, Ewa Tuszkiewicz-Misztal

  Medical science monitor : international medical journal of experimental and clinical research. 12/2011; 17(12):SR35-42.

  To evaluate the long-term safety and efficacy of etanercept treatment in Polish patients with juvenile idiopathic arthritis (JIA). The study involved patients, fulfilling the JIA criteria of theTo evaluate the long-term safety and efficacy of etanercept treatment in Polish patients with juvenile idiopathic arthritis (JIA). The study involved patients, fulfilling the JIA criteria of the International League of Associations of Rheumatology (ILAR), who were started on etanercept therapy after methotrexate and other synthetic disease-modifying antirheumatic drugs (DMARDs) had proven ineffective. Patient data were collected in an electronic registry. Disease improvement was assessed based on Giannini's criteria. The statistical analysis involved 188 patients. Significant improvement was observed in all clinical and laboratory parameters after the first month of therapy and was maintained in the following months. ACR Pediatric 30, 50, 70, 90, and 100 improvement was observed in 81.4%, 65.9%, 27.5%, 16.2%, and 15%, respectively, of patients after 3 months and in 94.7%, 88.4%, 62.1%, 34.7%, and 26.3%, respectively, after 24 months of treatment. Throughout the 72-month safety observation period, 1162 adverse events were reported; the exposure-adjusted AE rate was 2.96 per patient per year. In patients with various subtypes of JIA resistant to conventional DMARD treatment, etanercept resulted in significant and long-lasting improvements in disease activity. Combination treatment with etanercept and a DMARD was well tolerated.

• Identification, frequency, activation and function of CD4+ CD25(high)FoxP3+ regulatory T cells in children with juvenile idiopathic arthritis.

  Authors: Anna Stelmaszczyk-Emmel, Teresa Jackowska, Lidia Rutkowska-Sak, Maria Marusak-Banacka, Maria Wąsik

  Rheumatology international. 01/2011;

  The aim of the study was to test the frequency of CD4+ CD25(high)FoxP3 regulatory T cells in JIA patients and to assess their activation status and functional activity. The study involved 12 childrenThe aim of the study was to test the frequency of CD4+ CD25(high)FoxP3 regulatory T cells in JIA patients and to assess their activation status and functional activity. The study involved 12 children with JIA and 35 healthy control subjects. PBMC were stained with monoclonal antibodies (anti-CD25, anti-CD4, anti-CD127, anti-CD69, anti-CD71, and anti-FoxP3). The samples were evaluated using flow cytometer. CD4+ CD25- and CD4+ CD25+ cells were isolated by negative and positive selection with magnetic microbeads. CD4+ CD25+ and CD4+ CD25- cells were cultured separately and co-cultured (1:1) with or without PHA. The percentage of Tregs in JIA patients was significantly decreased in comparison with controls (median, 3.2 vs. 4.6; P = 0.042). Relative fluorescence intensities of FoxP3 were higher in JIA patients than in controls (median, 9.1 vs. 6.8). The percentage of activated Tregs (CD71+) was significantly higher in JIA patients in comparison with controls (median, 6.5 vs. 2.8; P = 0.00043). CD4+ CD25+ cells derived from JIA patients and controls were anergic upon PHA stimulation, while CD4+ CD25- cells showed intensive proliferative response. The proliferation rate of CD4+ CD25- cells stimulated by PHA was decreased in co-cultures. In JIA patients, the inhibition of proliferation of CD4+ CD25- cells by CD4+ CD25+ cells was 37.9%, whereas in controls it was significantly lower (55.7%, P = 0.046). JIA patients had statistically lower percentage of Tregs in peripheral blood compared to controls. CD4+ CD25+ cells sorted from peripheral blood of JIA patients had statistically lower ability to suppress CD4+ CD25- cell proliferation in comparison with cells obtained from controls.

• [Juvenile spondyloarthropaties].

  Authors: Lidia Rutkowska-Sak, Iwona Słowińska, Zbigniew Zuber

  Annales Academiae Medicae Stetinensis. 01/2010; 56 Suppl 1:29-33.

  Epidemiologic, clinical, and genetic studies provide grounds for differentiation of juvenile spondyloarthropathies from other rheumatic diseases. The prevalence of spondyloarthropathies among whitesEpidemiologic, clinical, and genetic studies provide grounds for differentiation of juvenile spondyloarthropathies from other rheumatic diseases. The prevalence of spondyloarthropathies among whites is estimated at 0.7 to 1.2% and the female-to-male ratio is 1:2.5. The current classification of clinically defined and undefined forms of juvenile spondyloarthropathies is presented together with their diagnostic criteria and treatment.

• [Nailfold videocapillaroscopy - a useful tool for screening patients with juvenile idiopathic arthritis at the risk of development of premature atherosclerosis].

  Authors: Anna Górska, Lidia Rutkowska-Sak, Elzbieta Musiej-Nowakowska, Sławomir Chlabicz, Stanisław Górski

  Postȩpy higieny i medycyny doświadczalnej (Online). 01/2010; 64:296-302.

  Inflammation of the vascular wall with endothelial dysfunction and subsequent activation of inflammatory immune response play pivotal roles in the early development of the atherosclerotic process notInflammation of the vascular wall with endothelial dysfunction and subsequent activation of inflammatory immune response play pivotal roles in the early development of the atherosclerotic process not only in adults with rheumatoid arthritis (RA), but also in children with juvenile idiopathic arthritis (JIA). This hypothesis was supported by our findings from autopsy examination, revealing atherosclerosis lesions in about 30% children with JIA. The established methods of assessing pre-clinical atherosclerosis include measurement of biochemical markers of endothelium impairment and ultrasonographic examination of vessels (FMD, IMT). The authors suggest that revealing structural and functional impairment of peripheral microvessels by means of static and dynamic videocapillaroscopy can give clinicians a chance to identify even younger patients with JIA/RA at high risk of atherosclerosis.

• Anaesthesiological problems in patients with rheumatoid arthritis undergoing orthopaedic surgeries.

  Authors: Barbara Lisowska, Lidia Rutkowska-Sak, Pawel Maldyk, Renata Cwiek

  Clinical rheumatology. 06/2008; 27(5):553-6.

  The article presents anaesthesiological problems in patients with rheumatoid arthritis (RA) scheduled for orthopaedic surgeries. Organ changes due to RA and related treatment were taken into account.The article presents anaesthesiological problems in patients with rheumatoid arthritis (RA) scheduled for orthopaedic surgeries. Organ changes due to RA and related treatment were taken into account. The anaesthetic techniques used for patients with RA underwent orthopaedic procedures were presented.

• [Spondyloarthropathies in childhood]

  Authors: Lidia Rutkowska-Sak

  Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego. 08/2004; 17(97):76-80.

  In this paper the author presents the own and other authors' current opinions about the classification, pathophysiology, clinical picture, differential diagnosis, treatment and prognostic problems inIn this paper the author presents the own and other authors' current opinions about the classification, pathophysiology, clinical picture, differential diagnosis, treatment and prognostic problems in the course of juvenile spondyloarthropathies.