Publications (5)0.76 Total impact
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Article: [Enhancing effects of perlecan or vascular endothelial growth factor-165 on angiogenesis in mice].
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ABSTRACT: To explore the angiogenesis-promoting effects of perlecan or vascular endothelial growth factor (VEGF) at different timepoints through quantitative analysis of microvessel density (MVD). Four kinds of scaffolds: open-cell polylatice acid (OPLA) (control), OPLA + VEGF-165, OPLA + perlecan and OPLA + VEGF-165 + perlecan were implanted into mice. At Weeks 1, 2, 3, 4, 6 and 8, OPLA was harvested and then HE and immunocytochemistry were employed to detect the angiogenesis-promoting effects of scaffold. MVD of each group was appraised by the Tukey test. The scaffolds exhibited excellent biocompatibility with tissue. Numerous vessels were spotted obviously around the implants, especially at Week 8. And the OPLA scaffold degraded with the elapsing of time and its inner part was divided into many sections along with the ingrowth of vessels. Compared with the other three groups, the MVD of the OPLA + VEGF-165 + perlecan group was the highest at all timepoints. There were statistical differences between the OPLA + VEGF-165 + perlecan and OPLA groups at Week 1 (3.30 ± 0.42 vs 1.80 ± 0.29); MVD of the OPLA + VEGF-165 + perlecan group was thrice as much as the OPLA group at Week 3 (11.70 ± 0.87 vs 4.50 ± 0.34); at Week 8, MVD of the OPLA + VEGF-165 + perlecan group was more than thrice as much as the OPLA group (31.40 ± 1.35 vs 9.90 ± 0.67). Angiogensis is synergistically enhanced by the combined application of VEGF-165 and perlecan in mice.Zhonghua yi xue za zhi 02/2012; 92(8):568-71. -
Article: Nanoleakage and microtensile bond strength at the adhesive-dentin interface after different etching times.
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ABSTRACT: To evaluate the effect of etching time on the nanoleakage and microtensile bond strength (microTBS) at the interface between etch-and-rise adhesives and dentin. Eighty molars were sectioned to expose the superficial dentin and randomly divided into four groups according to the adhesives used: OptiBond Solo (OB), Single Bond (SB), One-Step (OS), and Prime&Bond NT (PB). Within each group, a total-etching technique was applied, and the dentin surfaces were etched for 0, 15, 30 or 60 seconds. Each treated tooth was then sectioned into sticks. A non-trimming microTBS test and TEM observation for nanoleakage were sequentially performed. The degree of nanoleakage increased as etching time increased (P < 0.05). For the OS and PB groups, the highest microTBS values were achieved with 15 seconds of etching, followed by 30, 60 and 0 seconds. For the OB and SB groups, the microTBS values for 15- and 30-second etching times were similar and were both significantly higher than those of 0 and 60 seconds (P < 0.05).American journal of dentistry 12/2010; 23(6):335-40. · 0.76 Impact Factor -
Article: [The influence of nanometer inorganic filling carrying silver on the properties of composite resin].
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ABSTRACT: To observe the mechanical performance's effects of nanometer inorganic filling carrying silver on the properties of composite resin and find the perfect dose of the mixture. The resin was mixed with inorganic filling whose doses were 0.5%, 1.0%, 1.5% and 2.0%, and then the compressive strength, flexure strength and wear-resistance were measured in the five groups. One-way ANOVA and Student's t test were used for statistical analysis in SPSS 10.0. The results showed that in the compressive strength tests, the 1.5% dose was perfect which was (264.93+/-12.48) MPa (P>0.05) and the 2.0% dose was bad which was (94.54+/-17.42) MPa (P<0.01); in the flexure strength tests, 0.5%, 1.0% and 1.5% dose didn't change significantly which were (86.25+/-12.76) MPa, (99.49+/-16.47) MPa and (108.69+/-10.35) MPa (P>0.05) respectively, and 2.0% dose went down significantly which was (79.79+/-8.10) MPa (P<0.01); in the wear-resistance tests, 1.5% and 2.0% worked very well which were (2.73+/-0.53) MPa (P<0.05), (2.70+/-1.25) MPa (P<0.01) respectively. Nanometer inorganic filling had no significant influence on the composite resin, and 1.5% dose of nanometer inorganic filling is perfect for the resin's mechanical properties.Shanghai kou qiang yi xue = Shanghai journal of stomatology 03/2006; 15(1):73-6. -
Article: [The study on fabrication of dental restoration using PMMA-ZrO2 composites via CAD/CAM].
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ABSTRACT: To obtain dental restorations by machining PMMA-ZrO2 organic-inorganic composites with the dental CAD/CAM system. Partially sintered Zirconia compacts (PSZC) were prepared via isostatic pressing and partially sintering, with Zirconia nanopowder as raw materials. PMMA-Zirconia organic-inorganic composites were prepared by vacuum infiltrating the prepolymerized MMA into the PSZC, followed by in-situ polymerization. The mechanical properties and machinability of composites were studied. The composites were machined on the dental CAD/CAM system to obtain dental restoration. At 71.44% TD of PSZC, the composite had a 3-point bending strength of (202.56 +/- 3.09) MPa, fracture toughness of (4.30 +/- 0.16) MPa.m(1/2), elasticity modulus of (58.71 +/- 1.98) GPa, and Vickers hardness of (3.82 +/- 0.34) GPa, respectively. A premolar crown was fabricated by CAD/CAM system in 16 mins, and was verisimilitude, without any cracks. The composite at 71.44% TD of PSZC has good mechanical properties and dental restorations can be manufactured by PMMA-Zirconia composites via dental CAD/CAM system.Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology 02/2005; 40(1):23-6. -
Article: [A study of injectable autogenous tissue-engineered bone].
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ABSTRACT: To investigate the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection. Bone marrow cells harvested from iliac bone of New Zealand rabbits were induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mixed with 1.5% alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 4 x 10(9)/L. Calcium chloride was used as cross-linking agent to gel aqueous alginate solution. The marrow stromal osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of rabbits with autogenous cells transplantation. The samples were examined with X-ray and histological analysis. Four, eight and twelve weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals. On X-ray photograph the samples showed calcified image with more density than surrounding soft tissue, new bone formation was observed in the osteoblasts/alginate composites in histological analysis. The osteogenesis was in association with regenerated hematopoietic bone marrow. These results demonstrate that new bone tissue could be created through the injection of alginate sodium treated with autogenous marrow stromal osteoblasts.Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology 10/2003; 38(5):393-5.
