Publications (3)5 Total impact
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Article: Comparative investigation of elastic properties in a trabecula using micro-Brillouin scattering and scanning acoustic microscopy.
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ABSTRACT: Micro-Brillouin scattering (μ-BR) and a 200 MHz scanning acoustic microscope (SAM) with similar spatial resolutions were applied to evaluate tissue elastic properties in two directions in a trabecula. Acoustic impedance measured by SAM was in the range of 5-9 Mrayl. Wave velocities determined by μ-BR were in the range of (4.75-5.11) × 10(3) m/s. Both exhibited a similar trend of variation across the trabecula and were significantly correlated (R(2) = 0.63-0.67, p < 0.01). μ-BR is useful for the evaluation of tissue stiffness within a trabecula. Combined with SAM or nanoindentation, it can provide additional information to assess elastic anisotropy at the micro-scale.The Journal of the Acoustical Society of America 07/2012; 132(1):EL54-60. · 1.55 Impact Factor -
Article: Dependence of local wave velocity in bovine cortical bone on the decalcification.
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ABSTRACT: Bone is a composite material, mainly composed of HAp crystallites and type I collagen. It is known that the amount and orientation of HAp crystallites contribute to the "bone quality", which affects the bone elasticity. In this study, using a micro-Brillouin scattering technique which is able to evaluate wave velocity in the minute area, the effect of HAp amount on the velocity was measured. 36-plate-specimens in the plane of bone axis and radial directions were obtained from the middle part of a bovine femur. Wave velocity and HAp amounts were evaluated by the micro-Brillouin and XRD techniques, respectively. The specimens were then decalcified using ethylenediaminetetraacetic acid and measured again. Before decalcification, the average velocity was 5.06×10(3) m/s, and showed a moderate correlation with the HAp amounts (R(2)}=0.56). After decalcification, the average velocity dramatically decreased to the value of 3.28×10(3) m/s, showing a strong dependence on the HAp amounts. In addition, the wave velocities except for the lateral part shows the moderate correlation (R(2)}=0.30) before and after decalcification, which implies the possible effects of collagen on the wave velocities.The Journal of the Acoustical Society of America 04/2012; 131(4):3426. · 1.55 Impact Factor -
Article: Micro-Brillouin scattering measurements in mature and newly formed bone tissue surrounding an implant.
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ABSTRACT: The evolution of implant stability in bone tissue remains difficult to assess because remodeling phenomena at the bone-implant interface are still poorly understood. The characterization of the biomechanical properties of newly formed bone tissue in the vicinity of implants at the microscopic scale is of importance in order to better understand the osseointegration process. The objective of this study is to investigate the potentiality of micro-Brillouin scattering techniques to differentiate mature and newly formed bone elastic properties following a multimodality approach using histological analysis. Coin-shaped Ti-6Al-4V implants were placed in vivo at a distance of 200 μm from rabbit tibia leveled cortical bone surface, leading to an initially empty cavity of 200 μm×4.4 mm. After 7 weeks of implantation, the bone samples were removed, fixed, dehydrated, embedded in methyl methacrylate, and sliced into 190 μm thick sections. Ultrasonic velocity measurements were performed using a micro-Brillouin scattering device within regions of interest (ROIs) of 10 μm diameter. The ROIs were located in newly formed bone tissue (within the 200 μm gap) and in mature bone tissue (in the cortical layer of the bone sample). The same section was then stained for histological analysis of the mineral content of the bone sample. The mean values of the ultrasonic velocities were equal to 4.97×10(-3) m/s in newly formed bone tissue and 5.31×10(-3) m/s in mature bone. Analysis of variance (p=2.42×10(-4)) tests revealed significant differences between the two groups of measurements. The standard deviation of the velocities was significantly higher in newly formed bone than in mature bone. Histological observations allow to confirm the accurate locations of the velocity measurements and showed a lower degree of mineralization in newly formed bone than in the mature cortical bone. The higher ultrasonic velocity measured in newly formed bone tissue compared with mature bone might be explained by the higher mineral content in mature bone, which was confirmed by histology. The heterogeneity of biomechanical properties of newly formed bone at the micrometer scale may explain the higher standard deviation of velocity measurements in newly formed bone compared with mature bone. The results demonstrate the feasibility of micro-Brillouin scattering technique to investigate the elastic properties of newly formed bone tissue.Journal of Biomechanical Engineering 02/2011; 133(2):021006. · 1.90 Impact Factor
