Katarzyna Szewczyk

Jagiellonian University, Cracovia, Lesser Poland Voivodeship, Poland

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Publications (2)0 Total impact

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    ABSTRACT: The aim of the paper is to present the initial results of molecular examination which was started in 2006 for children with acute myeloid leukemia. Better knowledge of biology of this disease, can result in establishing of new risk factors what allows more precise patient stratification to different therapeutic groups. Study was obtained patients until to 18 years of age treated according to AML-BFM 2004 INTERIM protocol in 14 centers of the Polish Pediatric Leukemia/Lymphoma Study Group. Mononuclear cells were collected from bone marrow on time points established according to the AML-BFM 2004 INTERIM protocol. Collected cells were isolated on Ficoll gradient, and RNA and DNA were isolated using TRIZOL reagent. To synthesize cDNA an amount of 1 mg of total RNA was used. To perform quantitative RT-PCR and RQ-PCR reactions 4 fusion gene transcripts (AML1-ETO, CBFb-MYH11, PML-RARA /subtype bcrl and bcr3/) were used according to the protocol established by Europe Against Cancer Program. An expression of WT1 gene was tested additionally. An analysis of ABL control gene was used to normalize of achieved results. Determination of duplication of FLT3 gene in DNA sample was performed with starters complementary to JM region. Genotyping was performed in 75 patients with acute myeloid leukemia so far. AML1-ETO fusion gene transcript was found in 14 patients (19%). PML-RARA (subtype bcr3) and CBFB-MYH11 gene transcripts were detected in 3 (4%) and 3 (4%) patients, respectively. Duplication of FLT3 gene was found in 4 (5.3%) cases. Between 67 tested children over expression of WT1 was present in 51 patients (76%). Analysis of MRD level in subsequent time points showed systematic decrease of number of fusion gene transcript copies and gene WT1 expression. To establish the rate of molecular marker presence in AML in children and the influence of the presence of MRD on the treatment results as well, the study has to be conducted on a larger group of patients with longer follow-up.
    Przegla̧d lekarski 01/2010; 67(6):371-4.
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    ABSTRACT: The presence of disseminated neuroblastoma cells in bone marrow in children over 1 year old is important for clinical staging and risk assessment at diagnosis and for therapy monitoring. Reliable detection of single tumor cells in bone marrow may be a factor of great prognostic significance. Currently disseminated NBL cells are detected by conventional cytomorphological examination of bone marrow smears but this method is not sensitive enough to detect single tumor cells. The development of more sensitive methods of evaluation bone marrow is needed. For this purpose Neuroblastoma Bone Marrow Committee developed standard immunocytochemical assay based on detection of the neuroblastoma-specific antigen. Disialoganglioside GD2 is a surface antigen expressed on neuroblastoma cells but not detectable on the surface of normal bone marrow cells. This article describes significance of immunoctochemical method of identification neuroblastoma cells in bone marrow.
    Przegla̧d lekarski 01/2010; 67(6):409-12.