Kei Satoh

University of Texas MD Anderson Cancer Center, Houston, TX, United States

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Publications (3)10.57 Total impact

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    ABSTRACT: During normal mating in mammals between males and females, fertilization of oocytes by spermatozoa produces progeny with alleles inherited from both parents. Pluripotent embryonic stem (ES) cells can differentiate into all adult cell types, including oocytes and sperm. In culture, genetically male mouse ES lines spontaneously lose the Y chromosome at a frequency of about 1%, resulting in genetically female XO cell lines that can produce viable fertile females. Previous studies have not shown that such XY-derived XO pluripotent stem cells can generate viable progeny with genetic information from 2 distinct fathers. This present study exploited this stem cell strategy (in vitro sex reversal) to generate viable male and female mice that combine the haploid genomes from 2 fathers. Manipulation of fibroblasts from somatic cells of a male (XY) mouse fetus produced induced pluripotent stem cell lines. Injection of these XY-derived XO ES cells into blastocysts from donor female mice produced functional oocytes in female chimeras. Natural mating of the female chimeras (from father 1) with genetically distinct males (father 2) resulted in live progeny with haploid genomes that were equally derived from both fathers. These findings demonstrate that use of induced pluripotent stem cell technology can generate male and female progeny by combining the alleles from 2 male mice. This new method of mammalian reproduction may have major implications for improving livestock breeds and preserving endangered species as well as for advancing assisted reproductive technology in humans.
    Obstetrical and Gynecological Survey 03/2011; 66(4):216-218. · 2.51 Impact Factor
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    ABSTRACT: In sexual species, fertilization of oocytes produces individuals with alleles derived from both parents. Here we use pluripotent stem cells derived from somatic cells to combine the haploid genomes from two males to produce viable sons and daughters. Male (XY) mouse induced pluripotent stem cells (Father #1) were used to isolate subclones that had spontaneously lost the Y chromosome to become genetically female (XO). These male-derived XO stem cells were used to generate female chimeras that were bred with genetically distinct males (Father #2), yielding progeny possessing genetic information that was equally derived from both fathers. Thus, functional oocytes can be generated from male somatic cells after reprogramming and spontaneous sex reversal. These findings have novel implications for mammalian reproduction and assisted reproductive technology.
    Biology of Reproduction 03/2011; 84(3):613-8. · 4.03 Impact Factor
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    Biology of Reproduction 12/2010; · 4.03 Impact Factor

Publication Stats

2 Citations
10.57 Total Impact Points

Institutions

  • 2011
    • University of Texas MD Anderson Cancer Center
      • Department of Genetics
      Houston, TX, United States