Kana Sakai

Publications (2)2.27 Total impact

• Article: Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles.

  Kana Sakai, Yoshiya Hashimoto, Shunsuke Baba, Aki Nishiura, Naoyuki Matsumoto
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  ABSTRACT: We evaluated the effects on bone formation of combining synthesized collagen model polypeptides consisting of a Pro-Hyp-Gly [poly(PHG)] sequence and alpha-tricalcium phosphate (α-TCP) particles with various median sizes (large: 580.8 µm; small: 136.2 µm; or large and small mixed: 499.3 µm) in a skull defect model in mini-pigs. Quantitative image analyses for the volume density (VD) of new bone revealed that the VD in each α-TCP group was significantly higher than that in the poly(PHG) control group, with the mixed group showing the highest VD among all the groups at 4 weeks after implantation. Histological assessments revealed that the small α-TCP particles were almost completely degraded at 8 weeks. At 12 weeks, all sizes of α-TCP particles were completely degraded and remodeling of the lamellar bone was observed. The present findings suggest that particle size may influence the success of bone formation in defects.
  Dental Materials Journal 11/2011; · 1.14 Impact Factor
• Source

  Available from: nii.ac.jp

  Article: Effectiveness of scaffolds with pre-seeded mesenchymal stem cells in bone regeneration--assessment of osteogenic ability of scaffolds implanted under the periosteum of the cranial bone of rats.

  Shunsuke Baba, Takeomi Inoue, Yoshiya Hashimoto, Daisuke Kimura, Masatoshi Ueda, Kana Sakai, Naoyuki Matsumoto, Chiaki Hiwa, Taiji Adachi, Masaki Hojo
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  ABSTRACT: To date, there has been no study on the development of novel regimens based on the following tissue engineering principles: seeding and culturing mesenchymal stem cells (MSCs) on a scaffold before surgery or injecting cultured MSCs into a scaffold during surgery. The purpose of this study was to assess the in vivo osteogenic ability of scaffold/MSCs implanted beneath the periosteum of the cranial bone of rats in three different sample groups: one in which MSCs were pre-seeded and cultured on a scaffold to produce the 3-D woven fabric scaffold/MSC composite using osteo-lineage induction medium, one in which cultured MSCs produced by osteo-lineage induction in cell cultivation flasks were injected into a scaffold during surgery and a control group, in which only the 3-D woven fabric scaffold was implanted. The results indicate that pre-seeding MSCs on a scaffold leads to a higher osteogenic ability than injecting cultured MSCs into a scaffold during surgery.
  Dental Materials Journal 11/2010; 29(6):673-81. · 1.14 Impact Factor