Jong Hwan Yoon

Yeungnam University, Asan, South Chungcheong, South Korea

Are you Jong Hwan Yoon?

Claim your profile

Publications (12)36.24 Total impact

  • Jong Hwan Yoon, Young Jin Cho, Hyun Ho Park
    [Show abstract] [Hide abstract]
    ABSTRACT: The TNF receptor-associated factor (TRAF) proteins are structurally similar scaffold proteins that mediate between members of the TNF receptor (TNFR) family and downstream effector molecules such as kinases in the immune signalling pathway. Seven TRAFs have been identified, including TRAF4, which is a unique member that participates in many ontogenic processes, including nerve-system development. TRAFs commonly contain the TRAF domain, which mediates interaction with target receptors and effectors. As a first step towards elucidating the molecular mechanisms of the TRAF4-mediated signalling pathway, the first crystal structure of the human TRAF4 TRAF domain with a coiled-coil domain is reported at 2.3 Å resolution.
    Acta Crystallographica Section D Biological Crystallography 01/2014; 70(Pt 1):2-10. · 12.67 Impact Factor
  • Jong Hwan Yoon, Hyun Ho Park
    [Show abstract] [Hide abstract]
    ABSTRACT: TNF (tumour necrosis factor) receptor-associated factor 4 (TRAF4) is a unique TRAF protein that participates in morphogenetic and developmental function and cell migration. TRAF-family proteins contain a TRAF domain for target interaction. In this study, the short form of the human TRAF4 TRAF domain, corresponding to amino acids 290-462, was overexpressed in Escherichia coli using engineered C-terminal His tags. The short form of the TRAF4 TRAF domain was purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.2 Å from a crystal belonging to the hexagonal space group P32, with unit-cell parameters a = b = 147.17, c = 202.69 Å.
    Acta Crystallographica Section F Structural Biology and Crystallization Communications 08/2013; 69(Pt 8):916-919. · 0.55 Impact Factor
  • Jong Hwan Yoon, Hyun Ho Park
    [Show abstract] [Hide abstract]
    ABSTRACT: TNF receptor‐associated factor (TRAF) proteins were initially identified as tumour necrosis factor receptor (TNFR)‐interacting proteins that perform critical functions in the regulation of inflammation, antiviral responses and apoptosis. Although TRAF4 is a canonical TRAF protein, it contains a unique domain boundary and functions differently in the cell. In this study, the human TRAF4 TRAF domain, corresponding to amino acids 290–470, was overexpressed in Escherichia coli using engineered C‐terminal His tags. The TRAF4 TRAF domain was then purified to homogeneity and crystallized at 293 K. Finally, X‐ray diffraction data were collected to a resolution of 2.3 Å from a crystal belonging to space group P212121, with unit‐cell parameters a = 58.9, b = 87.9, c = 117.3 Å, α = β = γ = 90°.
    Acta Crystallographica Section F Structural Biology and Crystallization Communications 01/2013; 69(9). · 0.55 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Rab6A, a member of the Ras superfamily of small G proteins, is involved in the regulation of vesicle trafficking, which is critical for endocytosis, cell differentiation and cell growth. Rab6A can exist in two isoforms termed Rab6A and Rab6A'. The substitution of Gln72 by Leu (Q72L) in the Rab6A family blocks GTP-hydrolysis activity, and this mutation usually causes the Rab6A protein to be in a constitutively active form. In this study, in order to understand the functional uniqueness of Rab6A' and the molecular mechanism of the control of activity by GTP and GDP from the crystal structure, a Rab6A'(Q72L) mutant form was overexpressed in Escherichia coli with an engineered N-terminal His tag. Rab6A'(Q72L) was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 1.9 Å from a crystal belonging to space group P22(1)2(1) with unit-cell parameters a = 36.84, b = 96.78, c = 109.99 Å. The asymmetric unit was estimated to contain two molecules.
    Acta Crystallographica Section F Structural Biology and Crystallization Communications 09/2012; 68(Pt 9):1077-80. · 0.55 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The Ras small G protein-superfamily is a family of GTP hydrolases whose activity is regulated by GTP/GDP binding states. Rab6A, a member of the Ras superfamily, is involved in the regulation of vesicle trafficking, which is critical for endocytosis, biosynthesis, secretion, cell differentiation and cell growth. Rab6A exists in two isoforms, termed RabA and Rab6A'. Substitution of Gln72 to Leu72 (Q72L) at Rab6 family blocks GTP hydrolysis activity and this mutation usually causes the Rab6 protein to be constitutively in an active form. Here, we report the crystal structure of the human Rab6A'(Q72L) mutant form at 1.9Å resolution. Unexpectedly, we found that Rab6A'(Q72L) possesses GDP/Mg(2+) in the GTP binding pockets, which is formed by a flexible switch I and switch II. Large conformational changes were also detected in the switch I and switch II regions. Our structure revealed that the non-hydrolysable, constitutively active form of Rab6A' can accommodate GDP/Mg(2+) in the open conformation.
    Biochemical and Biophysical Research Communications 06/2012; 424(2):269-73. · 2.41 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The Death Domain (DD) superfamily, which is one of the largest classes of protein interaction modules, plays a pivotal role in apoptosis, inflammation, necrosis and immune cell signaling pathways. Because aberrant or inappropriate DD superfamily-mediated signaling events are associated with various human diseases, such as cancers, neurodegenerative diseases and immunological disorders, the studies in these fields are of great biological and clinical importance. To facilitate the understanding of the molecular mechanisms by which the DD superfamily is associated with biological and disease processes, we have developed the DD database (http://www.deathdomain.org), a manually curated database that aims to offer comprehensive information on protein-protein interactions (PPIs) of the DD superfamily. The DD database was created by manually curating 295 peer-reviewed studies that were published in the literature; the current version documents 175 PPI pairs among the 99 DD superfamily proteins. The DD database provides a detailed summary of the DD superfamily proteins and their PPI data. Users can find in-depth information that is specified in the literature on relevant analytical methods, experimental resources and domain structures. Our database provides a definitive and valuable tool that assists researchers in understanding the signaling network that is mediated by the DD superfamily.
    Nucleic Acids Research 12/2011; 40(Database issue):D331-6. · 8.81 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: β-Transaminase (β-TA) catalyzes the transamination reaction between β-aminocarboxylic acids and keto acids. This enzyme is a particularly suitable candidate for use as a biocatalyst for the asymmetric synthesis of enantiochemically pure β-amino acids for pharmaceutical purposes. The β-TA from Mesorhizobium sp. strain LUK (β-TAMs) belongs to a novel class in that it shows β-transaminase activity with a broad and unique substrate specificity. In this study, β-TAMs was overexpressed in Escherichia coli with an engineered C-terminal His tag. β-TAMs was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 2.5 Å from a crystal that belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 90.91, b = 192.17, c = 52.75 Å.
    Acta Crystallographica Section F Structural Biology and Crystallization Communications 02/2011; 67(Pt 2):231-3. · 0.55 Impact Factor
  • Source
    Journal of Physical Oceanography 01/2010; 40(2). · 3.18 Impact Factor
  • Sung Hyup You, Jong Hwan Yoon, Cheol-Ho Kim
    Terrestrial Atmospheric and Oceanic Sciences - TERR ATMOS OCEAN SCI. 01/2009; 20(5).
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The seasonal variation of eddy kinetic energy (EKE) of the North Pacific Subtropical Countercurrent (STCC) was investigated by analyzing the data from an eddy-resolving OGCM with the horizontal resolution 1/12° in comparison with satellite altimetry data. Although the annual cycle of simulated EKE of the whole STCC domain showed agreement with satellite data, with a maximum in spring and a minimum in fall, it revealed latitudinal dependence; that is, near 19°N the EKE level is higher in summer than in winter, but it is higher in winter than in summer north of 20°N. The OGCM also reproduced two branches of the STCC, which affect the EKE variation. The variation of EKE level was shown to be closely related to the growth rate of baroclinic eddies. The simulated zonal velocity is much larger than climatological value, thus allowing much faster growth of baroclinic eddies.
    Geophysical Research Letters 01/2007; 34(7). · 3.98 Impact Factor
  • Jong‐Hwan Yoon, Choochon Lee
    [Show abstract] [Hide abstract]
    ABSTRACT: Annealing effects on electron drift mobility are investigated using time‐of‐flight photocurrent measurements in hydrogenated amorphous silicon. It is found that at room temperature the electron drift mobility and dispersion parameters decrease as annealing temperature is increased, but mobility activation energy remains unchanged by annealing treatments. These results can be explained by the broadening of exponential band tail due to the annealing process.
    Journal of Applied Physics 06/1985; · 2.21 Impact Factor
  • Sung Hyup You, Jong Hwan Yoon
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study developed Pacific Ocean models from the Research Institute for Applied Mechanics Ocean Model (RIAMOM) with very high horizontal (1/6° and 1/12°) and vertical (70 levels) resolutions. The hydrographic features of the simulations show good agreement with observed climatological features. Solution differences between the 1/6° and 1/12° models are small for general features of various physical components, but large for eddy fields and the strengths of western boundary currents and their extensions. However, the two high-resolution models show realistic climatological features of Pacific Ocean circulation patterns. Volume transports through major straits in the northwestern Pacific Ocean were also simulated and compared with previous observational results. Key wordsHigh-resolution model-Pacific Ocean model-RIAMOM
    Asia-Pacific Journal of the Atmospheric Sciences 46(1):97-112. · 0.78 Impact Factor

Publication Stats

24 Citations
36.24 Total Impact Points

Institutions

  • 2011–2013
    • Yeungnam University
      • School of Biotechnology
      Asan, South Chungcheong, South Korea
    • Myongji University
      • Department of Computer Engineering
      Seoul, Seoul, South Korea
  • 2012
    • Seoul National University Hospital
      Sŏul, Seoul, South Korea
  • 1985
    • Korea Advanced Institute of Science and Technology
      • Department of Physics
      Seoul, Seoul, South Korea