Jingmei Lu

Northeast Normal University, Hsin-ching, Jilin Sheng, China

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Publications (7)9.78 Total impact

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    ABSTRACT: A new compound based on polyoxometalates (POMs) and the quinolone antibacterial pipemidic acid (HPPA), {[Co(PPA)2]H2[ SiW12O40]}·HPPA·3H2O (1), has been prepared and characterized by elemental analyses, IR and singe crystal X-ray diffraction. The title compound represents the first example of POMs modified by the quinolone antibacterial HPPA, in which the POMs are modified by [Co(HPPA)2] subunits. Its antitumor activity on MCF-7 cells was investigated by the. The results show that the title compound exhibits higher antitumor activity than its parent, which indicates that introduction of M-PPA into the POM surface can increase their antitumor activity and make the compounds to penetrate into the cells easily.
    Inorganic Chemistry Communications - INORG CHEM COMMUN. 01/2011; 14(8):1192-1195.
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    ABSTRACT: In the present study, the water-soluble polysaccharides of Radix Aconiti, Radix Aconiti Lateralis and Radix Aconiti Kusnezoffii, were extracted and fractionated into four fractions of each material. The FT-IR and chemical analyses indicated the water-soluble polysaccharides of the three materials were all mainly composed of starch, non-starch type alpha-D-glucans and pectic polysaccharides with different molecular weight distributions and monosaccharide composition ratios. The antitumor assay showed that all the non-starch type polysaccharide fractions had good antitumor activities, and the tumor growth inhibition ratios were 37.24-70.42%. Specifically the inhibition ratios of pectic polysaccharides were over 60%. Moreover, the immunological tests using the Cyclophosphamide (Cy) induced immunosuppressive mice, including phagocytosis of macrophage, NK cell activity, concanavalin A (ConA)-induced T-cell proliferation, lipopolysaccharide (LPS)-induced B-cell proliferation, quantitative haemolysis of sheep red blood cells (SRBC) and dinitro-fluorobenzene (DNFB)-induced delayed-type hypersensitivity (DTH) response assays, exhibited that all the non-starch type polysaccharides, especially the pectic polysaccharide fractions, not only had remarkable immunostimulating activities including nonspecific immunity, cellular immunity and humoral immunity, but also could restore the antitumor drug-suppressed immune function. Therefore, the polysaccharides from Aconitum species might be conveniently exploited to be good immune stimulating modifiers and had the potential to apply in the tumor therapy.
    Natural product communications 03/2010; 5(3):447-55. · 0.96 Impact Factor
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    ABSTRACT: Aconitum kusnezoffii Reichb. has been used as traditional Chinese medicine over the last 2500 years, but its polysaccharides have been paid little attention by now. In the present study, a hot alkali extracted polysaccharide (AKP) from A. kusnezoffii Reichb. was characterized to be an alpha-(1-->3),(1-->4)-D-glucan with Mw 1.4 x 10(5) Da, of which (1-->3)-linked and (1-->4)-linked alpha-Glcp residues were in a ratio of 1:7. In vitro antioxidant testing indicated that AKP had significant ferrous ion-chelating ability, reducing power and scavenging effects on DPPH radical, hydroxyl radical, superoxide anion, H2O2 and self-oxidation of 1,2,3-phentriol, suggesting that it should be explored as a novel natural antioxidant.
    International journal of biological macromolecules 10/2009; 46(1):85-90. · 2.37 Impact Factor
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    ABSTRACT: A strain SH 2016, capable of producing xylanase, was isolated and identified as Aspergillus awamori, based on its physiological and biochemical characteristics as well as its ITS rDNA gene sequence analysis. A xylanase gene of 591 bp was cloned from this newly isolated A. awamori and the ORF sequence predicted a protein of 196 amino acids with a molecular mass about 21 kDa. An expression plasmid carrying the gene under the control of the methanol regulated alcohol oxidase gene (AOX1) promotor was introduced into Pichia pastoris, and xylanase gene was successfully expressed into the medium using methanol as inducer. Xylanase with 6 his tags was purified using Ni2+-NTA column. The characteristics of purified xylanase were investigated.
    AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2009; 7:4251-4259. · 0.57 Impact Factor
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    ABSTRACT: The ferric uptake regulator (Fur) is a predominant bacterial regulator controlling the iron assimilation functions in response to iron availability. Our previous microarray analysis on Yersinia pestis defined the iron-Fur modulon. In the present work, we reannotated the iron assimilation genes in Y. pestis, and the resulting genes in complementation with those disclosed by microarray constituted a total of 34 genome loci (putative operons) that represent the potential iron-responsive targets of Fur. The subsequent real-time reverse transcription-PCR (RT-PCR) in conjunction with the primer extension analysis showed that 32 of them were regulated by Fur in response to iron starvation. A previously predicted Fur box sequence was then used to search against the promoter regions of the 34 operons; the homologue of the above box could be predicted in each promoter tested. The subsequent electrophoretic mobility shift assay (EMSA) demonstrated that a purified His(6) tag-fused Fur protein was able to bind in vitro to each of these promoter regions. Therefore, Fur is a global regulator, both an activator and a repressor, and directly controls not only almost all of the iron assimilation functions but also a variety of genes involved in various non-iron functions for governing a complex regulatory cascade in Y. pestis. In addition, real-time RT-PCR, primer extension, EMSA, and DNase I footprinting assay were used to elucidate the Fur regulation of the ybt locus encoding a virulence-required iron uptake system. By combining the published data on the YbtA regulation of ybt, we constructed a concise Fur/YbtA regulatory network with a map of the Fur-promoter DNA interactions within the ybt locus. The data presented here give us an overview of the iron-responsive Fur regulon in Y. pestis.
    Journal of bacteriology 05/2008; 190(8):3063-75. · 3.94 Impact Factor
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    Tao Zhang, Jingmei Lu
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    ABSTRACT: The title compound, [Nd(OH)(SO(4))](n), was obtained hydro-thermally from an aqueous solution of neodymium nitrate, 1,2-propane-diamine and sulfuric acid. The structure features nona-coordinated neodymium with sulfate and hydroxide anions acting as bridging ligands. The OH group forms a weak O-H⋯O hydrogen bond with an O⋯O distance of 3.224 (5) Å.
    Acta Crystallographica Section E Structure Reports Online 01/2008; 64(Pt 8):i49. · 0.35 Impact Factor
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    ABSTRACT: Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants.
    Science in China Series C Life Sciences 09/2006; 49(4):305-10. · 1.61 Impact Factor