Jingmei Lu

Northeast Normal University, Hsin-ching, Jilin Sheng, China

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Publications (14)19.41 Total impact

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    ABSTRACT: By-products originating from food processing are a considerable disposal problem for the food industry. Because of the absence of specifically effective processing technology, huge quantities of by-products are often abandoned as rubbish and prone to microbial spoilage. Given this, a simultaneous microwave/ultrasonic assisted enzymatic extraction (SMU-AEE) method was established for the first time, and performed for antioxidant ingredients extraction from Nitraria tangutorum juice by-products (NJB) in the present study. Its experimental conditions were optimized by single factor test and response surface methodology (RSM), and gave the corresponding response values for antioxidant capacity of NJB extract (NJBE) of 219.73 ± 7.03 mg TE/g, which was 27.62%–190.23% higher than those obtained by traditional extraction methods. Chemical composition assay suggested that the increasing of antioxidant capacity of NJBE by SMU-AEE was because of the improvement of extraction efficiency of antioxidant ingredients from NJB, including phenols, flavonoids and anthocyanins. Furthermore, oxidative injury protection ability assay showed that NJBE was good at protecting cells from UVB-oxidative phototoxicity and doxorubicin-oxidative cardiotoxicity, and its protecting ability surpasses or approaches to that of grape seed extract (GSE, the positive control drug), indicating its good potential to be a natural antioxidant in food and pharmaceutical industries.
    Industrial Crops and Products 04/2015; 66. DOI:10.1016/j.indcrop.2014.12.054 · 3.21 Impact Factor
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    ABSTRACT: A new compound based on polyoxometalates (POMs) and the quinolone antibacterial pipemidic acid (HPPA), {[Co(PPA)2]H2[ SiW12O40]}·HPPA·3H2O (1), has been prepared and characterized by elemental analyses, IR and singe crystal X-ray diffraction. The title compound represents the first example of POMs modified by the quinolone antibacterial HPPA, in which the POMs are modified by [Co(HPPA)2] subunits. Its antitumor activity on MCF-7 cells was investigated by the. The results show that the title compound exhibits higher antitumor activity than its parent, which indicates that introduction of M-PPA into the POM surface can increase their antitumor activity and make the compounds to penetrate into the cells easily.
    Inorganic Chemistry Communications 08/2011; 14(8):1192-1195. DOI:10.1016/j.inoche.2011.03.075 · 2.06 Impact Factor
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    ABSTRACT: In the present study, the water-soluble polysaccharides of Radix Aconiti, Radix Aconiti Lateralis and Radix Aconiti Kusnezoffii, were extracted and fractionated into four fractions of each material. The FT-IR and chemical analyses indicated the water-soluble polysaccharides of the three materials were all mainly composed of starch, non-starch type alpha-D-glucans and pectic polysaccharides with different molecular weight distributions and monosaccharide composition ratios. The antitumor assay showed that all the non-starch type polysaccharide fractions had good antitumor activities, and the tumor growth inhibition ratios were 37.24-70.42%. Specifically the inhibition ratios of pectic polysaccharides were over 60%. Moreover, the immunological tests using the Cyclophosphamide (Cy) induced immunosuppressive mice, including phagocytosis of macrophage, NK cell activity, concanavalin A (ConA)-induced T-cell proliferation, lipopolysaccharide (LPS)-induced B-cell proliferation, quantitative haemolysis of sheep red blood cells (SRBC) and dinitro-fluorobenzene (DNFB)-induced delayed-type hypersensitivity (DTH) response assays, exhibited that all the non-starch type polysaccharides, especially the pectic polysaccharide fractions, not only had remarkable immunostimulating activities including nonspecific immunity, cellular immunity and humoral immunity, but also could restore the antitumor drug-suppressed immune function. Therefore, the polysaccharides from Aconitum species might be conveniently exploited to be good immune stimulating modifiers and had the potential to apply in the tumor therapy.
    Natural product communications 03/2010; 5(3):447-55. · 0.92 Impact Factor
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    ABSTRACT: Aconitum kusnezoffii Reichb. has been used as traditional Chinese medicine over the last 2500 years, but its polysaccharides have been paid little attention by now. In the present study, a hot alkali extracted polysaccharide (AKP) from A. kusnezoffii Reichb. was characterized to be an alpha-(1-->3),(1-->4)-D-glucan with Mw 1.4 x 10(5) Da, of which (1-->3)-linked and (1-->4)-linked alpha-Glcp residues were in a ratio of 1:7. In vitro antioxidant testing indicated that AKP had significant ferrous ion-chelating ability, reducing power and scavenging effects on DPPH radical, hydroxyl radical, superoxide anion, H2O2 and self-oxidation of 1,2,3-phentriol, suggesting that it should be explored as a novel natural antioxidant.
    International journal of biological macromolecules 10/2009; 46(1):85-90. DOI:10.1016/j.ijbiomac.2009.10.011 · 3.10 Impact Factor
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    ABSTRACT: Morphology and culture studies on germlings of Sargassum thunbergii (Mertens et Roth) Kuntze were carried out under controlled laboratory conditions. Growth characteristics of these germlings grown under different temperatures (from 10 to 25°C), irradiances (from 9 to 88μmol photons m−2 s−1), and under blue and white light conditions are described. The development of embryonic germlings follows the classic “8 nuclei 1 egg” type described for Sargassaceae. Fertilized eggs spent 5–6h developing into multicellular germlings with abundant rhizoids after fertilization. Under conditions of 20°C, 44μmol photons m−2 s−1 and photoperiod of 12h, young germlings with one or two leaflets reached 2–3mm in length after 8weeks. Temperature variations (10, 15, 20, 25°C) under 88μmol photons m−2 s−1 significantly influenced the growth rate within the first week, although this effect became less obvious after 8weeks, especially at 15 and 20°C. Variation in germling growth was highly significant under different irradiances (9, 18, 44, 88μmol photons m−2 s−1) at 25°C. Low temperature (10°C) reduced germling growth. Growth of germlings cultured under blue light was lower than in white light. Optimal growth of these germlings occurred at 25°C and 44μmol photons m−2 s−1.
    Journal of Applied Phycology 02/2009; 20(5):925-931. DOI:10.1007/s10811-008-9311-y · 2.49 Impact Factor
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    ABSTRACT: A strain SH 2016, capable of producing xylanase, was isolated and identified as Aspergillus awamori, based on its physiological and biochemical characteristics as well as its ITS rDNA gene sequence analysis. A xylanase gene of 591 bp was cloned from this newly isolated A. awamori and the ORF sequence predicted a protein of 196 amino acids with a molecular mass about 21 kDa. An expression plasmid carrying the gene under the control of the methanol regulated alcohol oxidase gene (AOX1) promotor was introduced into Pichia pastoris, and xylanase gene was successfully expressed into the medium using methanol as inducer. Xylanase with 6 his tags was purified using Ni2+-NTA column. The characteristics of purified xylanase were investigated.
    AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2009; 7:4251-4259. · 0.57 Impact Factor
  • Acta Crystallographica Section E Structure Reports Online 09/2008; 64(9). DOI:10.1107/S1600536808025543/lh2667sup0.html · 0.35 Impact Factor
  • Tao Zhang, Jingmei Lu
    Acta Crystallographica Section E Structure Reports Online 08/2008; 64(8). DOI:10.1107/S1600536808021818/br2079Isup2.hkl · 0.35 Impact Factor
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    Tao Zhang, Jingmei Lu
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    ABSTRACT: The title compound, [Nd(OH)(SO(4))](n), was obtained hydro-thermally from an aqueous solution of neodymium nitrate, 1,2-propane-diamine and sulfuric acid. The structure features nona-coordinated neodymium with sulfate and hydroxide anions acting as bridging ligands. The OH group forms a weak O-H⋯O hydrogen bond with an O⋯O distance of 3.224 (5) Å.
    Acta Crystallographica Section E Structure Reports Online 07/2008; 64(Pt 8):i49. DOI:10.1107/S1600536808021818 · 0.35 Impact Factor
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    ABSTRACT: The ferric uptake regulator (Fur) is a predominant bacterial regulator controlling the iron assimilation functions in response to iron availability. Our previous microarray analysis on Yersinia pestis defined the iron-Fur modulon. In the present work, we reannotated the iron assimilation genes in Y. pestis, and the resulting genes in complementation with those disclosed by microarray constituted a total of 34 genome loci (putative operons) that represent the potential iron-responsive targets of Fur. The subsequent real-time reverse transcription-PCR (RT-PCR) in conjunction with the primer extension analysis showed that 32 of them were regulated by Fur in response to iron starvation. A previously predicted Fur box sequence was then used to search against the promoter regions of the 34 operons; the homologue of the above box could be predicted in each promoter tested. The subsequent electrophoretic mobility shift assay (EMSA) demonstrated that a purified His(6) tag-fused Fur protein was able to bind in vitro to each of these promoter regions. Therefore, Fur is a global regulator, both an activator and a repressor, and directly controls not only almost all of the iron assimilation functions but also a variety of genes involved in various non-iron functions for governing a complex regulatory cascade in Y. pestis. In addition, real-time RT-PCR, primer extension, EMSA, and DNase I footprinting assay were used to elucidate the Fur regulation of the ybt locus encoding a virulence-required iron uptake system. By combining the published data on the YbtA regulation of ybt, we constructed a concise Fur/YbtA regulatory network with a map of the Fur-promoter DNA interactions within the ybt locus. The data presented here give us an overview of the iron-responsive Fur regulon in Y. pestis.
    Journal of bacteriology 05/2008; 190(8):3063-75. DOI:10.1128/JB.01910-07 · 2.69 Impact Factor
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    ABSTRACT: The asymmetric unit of the title compound, C(27)H(32)O, contains two and a half mol-ecules. In the crystal structure, one of the mol-ecules lies on a crystallographic twofold rotation axis. The dihedral angles between the benzene rings are 12.17 (6), 16.29 (11) and 51.24 (8)° for the three molecules. The dihedral angles between the benzene rings of each molecule in the asymmetric unit are 12.17 (6) and 16.29 (11)°
    Acta Crystallographica Section E Structure Reports Online 01/2008; 64(Pt 9):o1755. DOI:10.1107/S1600536808025543 · 0.35 Impact Factor
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    ABSTRACT: Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants.
    Science in China Series C Life Sciences 09/2006; 49(4):305-10. DOI:10.1007/s11427-006-0305-1 · 1.61 Impact Factor
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    ABSTRACT: A scanning electron microscope observation is carried out onGlycine soja L., which grows around the estuary of the Yellow River, in Kenli County, Shandong Province. It is first discovered that there are salt glands, in the shape of balls, on the surface of the stems and leaves ofG. soja L., and distributed at the intercellular layer. With a handle at the base, the salt glands are not equal in size, and show a layer-upon-layer structure. They have a diameter of 21.6 μm, a handle length of 1.2 μm, their secretory pore’s diameter is 5.6 μm. There are two ways to secrete salt ions: younger glands secrete salt ions through the secretory pore, and mature glands by breaking themselves.
    Chinese Science Bulletin 04/1999; 44(10):923-926. DOI:10.1007/BF02885066 · 1.37 Impact Factor
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    ABSTRACT: This study investigates the relationship between the induction of MCF-7 human breast cancer cell apoptosis by Rhizoma Paridis saponins (RPS) and the mitochondrial apoptotic pathway. We treated MCF-7 cells with RPS at various concentrations and examined the inhibitory effect of RPS on the proliferation of MCF-7 cells using the 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the change in mitochondrial membrane potential (MMP) using JC-1 staining, and the expression levels of related proteins using western blot. The results showed that the inhibitory effect of RPS against the growth of MCF-7 human breast cancer cells might be related to the apoptosis induced caspase family and the caspase-3-dependent pathway. The results suggest that RPS has the potential to be a valuable anticancer agent.