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Tissue Antigens 06/2013; · 2.59 Impact Factor
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ABSTRACT: HLA-B*49:24 shows one nucleotide difference regarding B*49:10 at codon 12 (ATG>GTG). DRB1*03:64 differs from DRB1*03:01:01 in one amino acid residue at position 59, E>Q.
Tissue Antigens 01/2013; · 2.59 Impact Factor
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ABSTRACT: B*44:150 was identical to B*44:02:01:01 except at the 3'-end region of exon 3, where a B*07-specific sequence was identified.
Tissue Antigens 12/2012; 80(6):548-9. · 2.59 Impact Factor
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ABSTRACT: Two novel HLA-B alleles were characterized. HLA-B*37:34 shows two nucleotide differences regarding B*37:10 at codons 79 (CGC>CGG) and 80 (ACC>ATC), resulting in one amino acid replacement at position 80 (T>I). HLA-B*44:152 differs from B*44:02:01 in one nucleotide at codon 81 (GCG>ACG) giving rise to a leucine to threonine substitution at position 81.
Tissue Antigens 10/2012; · 2.59 Impact Factor
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ABSTRACT: Two novel HLA-A29 alleles were described. A*29:39 differs from A*29:02:01 by three clustered amino acid replacements at α1 domain, T73>I73, A76>V76 and N77>D77. A*29:40 shows one nucleotide difference regarding A*29:02:01 allele, resulting in one amino acid substitution at position 154, E154>G154.
Tissue Antigens 10/2012; · 2.59 Impact Factor
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ABSTRACT: B*08:79 is composed by partial B*08:01:01 and B*07:06 sequences because of a possible recombination event within intron 2.
Tissue Antigens 06/2012; 80(3):268-70. · 2.59 Impact Factor
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ABSTRACT: A*33:49 has one nucleotide change regarding A*33:01:01 at exon 3, producing an amino acid replacement at codon 97, M97 to I97.
Tissue Antigens 04/2012; 80(1):65-6. · 2.59 Impact Factor
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ABSTRACT: To determine the complete sequence of a newly identified human leukocyte antigen (HLA)-C allele, we designed a method where the full genomic sequence of HLA-C*04 was amplified in isolation from the patient second HLA-C allele in a single polymerase chain reaction (PCR), using primers spanning its 5'- and 3'-untranslated regions. The new allele, officially designated HLA-C*04:71, differs from HLA-C*04:01:01:01 by two single-nucleotide polymorphisms: one determines substitution of phenylalanine for serine 9 at the B pocket of the peptide-binding site; the second substitution is a new polymorphism in intron 5. Phe-9 is characteristic of Cw1 alleles and its presence in C*04:71 most likely affects its peptide-binding repertoire. The principle we have used for C*04:71 isolation could be adapted for unambiguous sequence-based HLA-C typing of selected samples in a clinical setting.
Tissue Antigens 04/2012; 79(4):291-4. · 2.59 Impact Factor
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ABSTRACT: Graft engineering procedures for hematopoietic SCT (HSCT) may improve the chance of success in matched unrelated donor (MUD) and haploidentical donor transplantations. Successful donor immune reconstitution is important to mediate GVL effects in reduced-intensity conditioning (RIC) HSCT. We prospectively investigated early immune reconstitution and clinical outcome in 30 CD3/CD19-depleted MUD (n=15) or HP (n=15) HSCTs for high-risk childhood leukemia using a fludarabine-based RIC without serotherapy. The graft consisted of a mean of 10.5 × 10(6)/kg CD34+, 77 × 10(3)/kg CD3+ and 39 × 10(6)/kg CD56+ cells. After transplantation, 86% of the patients engrafted. In all, 13% of patients had >grade 3 acute GVHD. Natural killer (NK) cell, DC and T-cell recovery achieved normal values within the first 60 days after transplantation. DC recovery was dominated by the DC2(-) subset. NK-cell phenotype was altered and cytotoxicity was lower compared with their donors. EFS was 50±9% (73±11% for those in CR1 and 26±11% for those with advanced disease). Faster DC2(-) recovery was associated with better outcome, especially in the MUD setting. In summary, CD3/CD19-depleted HSCT with fludarabine-based RIC without serotherapy resulted in favorable patient survival, and rapid NK, DC and T-cell recovery.Bone Marrow Transplantation advance online publication, 12 March 2012; doi:10.1038/bmt.2012.43.
Bone marrow transplantation 03/2012; · 3.00 Impact Factor
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ABSTRACT: DRB1*04:98 shows four nucleotide changes regarding DRB1*04:01:01, resulting in two amino acids replacement at positions A73G and A74R.
Tissue Antigens 07/2011; 78(6):462-3. · 2.59 Impact Factor
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ABSTRACT: The novel HLA-B*44:130 allele was found in a Spanish donor. B*44:130 differs from B*44:40 by four nucleotide changes at codons 11, 12 and 24, producing three amino acid replacements, 11A>S, 12M>V and 24T>S.
Tissue Antigens 06/2011; 78(5):398-9. · 2.59 Impact Factor
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ABSTRACT: Sequencing-based typing of HLA-DRB1 gene, allowed us to characterize three new alleles. DRB1*03:55 shows one nucleotide change regarding DRB1*03:01:01G, resulting in an amino acid replacement at position 80 R > I. DRB1*11:46:02 presents one synonymous nucleotide change at codon 34 (CAG > CAA) with regard to DRB1*11:46:01. Finally, DRB1*04:92 has one nucleotide change from DRB1*04:07:01 at codon 207 in exon 4, producing an amino acid replacement (V > M) in the transmembrane domain.
International Journal of Immunogenetics 06/2011; 38(5):435-6. · 1.29 Impact Factor
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Tissue Antigens 03/2011; 78(1):72-3. · 2.59 Impact Factor
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ABSTRACT: Histocompatibility criteria for unrelated donor selection are based on high-resolution definition of HLA genes. In spite of the expansion of the unrelated donor registries, HLA matching remains a problem for many patients because of the great diversity of HLA alleles and haplotypes. The availability of matched donors at an allelic level depends on the frequency of the patient's alleles and haplotypes. Therefore, data regarding HLA distribution for each population are needed in order to evaluate the donor searching approach and, may be, even the therapeutic strategy. In the present report, we have analyzed 253 haematological Spanish patients awaiting unrelated haematopoietic stem cell (HSC) donors. HLA allele and haplotype frequencies have been defined at high resolution for the first time in this population. Significant differences in HLA distribution have been reported when comparing two patient groups, one that received full-match (10/10) unrelated donors and one that did not. Factors like rare alleles, presence of B*510101 (because of the association with multiple HLA-C alleles), as well as infrequent B-C and DRB1-DQB1 associations, showed a negative value for finding a suitable donor, whereas the presence of one of the six-gene haplotypes with a frequency ≥ 0.9% in our sample was a positive factor influencing donor searching. These differences will be useful in donor searching advising and in the use of different therapeutic strategies.
Tissue Antigens 01/2011; 77(1):45-53. · 2.59 Impact Factor
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ABSTRACT: Human leukocyte antigen (HLA)-DRB1*1615 shows one amino acid replacement at codon 86 (G>V) regarding DRB1*160101.
Tissue Antigens 03/2010; 75(3):288-9. · 2.59 Impact Factor
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ABSTRACT: Human leukocyte antigen (HLA)-B*4907 was detected in a family from Morocco because of inconsistencies found between molecular and serologic typing results. B*4907 differs from B*490101 by two nucleotide changes in codon 163, producing an amino acid replacement, L>E.
Tissue Antigens 03/2010; 75(3):286-7. · 2.59 Impact Factor
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ABSTRACT: Human leukocyte antigen (HLA)-DQA1*0510 has been identified in a Spanish patient diagnosed with celiac disease. DQA1*0510 differs from DQA1*0505/09 by a point mutation at exon 2 producing an amino acid replacement at codon 77 (I>V).
Tissue Antigens 03/2010; 75(3):287-8. · 2.59 Impact Factor
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ABSTRACT: Human leukocyte antigen (HLA) class I sequence-based typing (SBT) for hematopoietic unrelated donor searching in a Romanian Caucasian patient showed the presence of a novel HLA-B allele defined as B*0777. HLA-B*0777 has two nucleotides changes at the same codon from B*0707, resulting an amino acid replacement 99Y > 99S.
Tissue Antigens 10/2009; 74(6):543-4. · 2.59 Impact Factor
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Tissue Antigens 09/2009; 74(6):553-4. · 2.59 Impact Factor
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ABSTRACT: Sequencing-based typing for unrelated hematopoietic stem cell transplantation studies rendered four new HLA-C alleles. HLA-Cw*0757, Cw*070403, and Cw*1705 differ by single point mutations from their most similar alleles, Cw*070105, Cw*070401, and Cw*1701, respectively. In contrast, Cw*0223 discloses five amino acid replacements at the alpha2 domain regarding Cw*020202.
Tissue Antigens 07/2009; 73(6):618-20. · 2.59 Impact Factor
