Hui Li

Sun Yat-Sen University, Shengcheng, Guangdong, China

Are you Hui Li?

Claim your profile

Publications (144)382.42 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Sepsis, a major clinical problem with high morbidity and mortality, is caused by overwhelming systemic host-inflammatory response. Toll-like receptors (TLRs) play a fundamental role in induction of hyperinflammation and tissue damage in sepsis. In this study, we demonstrate a protective role of TLR9 inhibition against the dysregulated inflammatory response and tissue injury in sepsis. TLR9 deficiency decreased the mortality of mice following cecal ligation and puncture (CLP)-induced sepsis. TLR9 knockout mice showed dampened p38 activation and augmented Akt phosphorylation in the spleen, lung and liver. In addition, TLR9 deficiency decreased the levels of inflammatory cytokines and attenuated splenic apoptosis after CLP. These results indicate that TLR9 inhibition might offer a novel therapeutic strategy for the management of sepsis. Copyright © 2015 Elsevier Inc. All rights reserved.
    Cellular Immunology 03/2015; 295(2). DOI:10.1016/j.cellimm.2015.03.009 · 1.87 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: PcPAF is a novel antifungal protein identified by our recent study, which is produced by a fungal strain Penicillium citrinum W1 isolated from a Southwest Indian Ocean sediment sample. The present study identified glycine as a potential metabolite which increased the fungal growth and promoted antifungal activity. Then, GC/MS based metabolomics was used to disclose the metabolic mechanism manipulated by glycine. With the aid of unsupervised hierarchical clustering analysis (HCA) and supervised orthogonal partial least squares discriminant analysis (OPLS-DA), the intracellular metabolite profiles were distinguished among two glycine-treated groups and control. 43 and 47 significantly varied metabolites were detected in 2.5 mM or 5 mM glycine-treated groups and involved in seven and eight pathways, respectively. Furthermore, exogenous serine, which is converted from glycine, showed the same potential as glycine did. Our findings not only identify glycine and serine as nutrients which promoted P. citrinum W1 growth and increased antifungal activity, but also highlight the way to utilize metabolomics for an understanding of metabolic mechanism manipulated by an exogenous compound. © FEMS 2015. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
    FEMS Microbiology Letters 03/2015; DOI:10.1093/femsle/fnv040 · 2.72 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Edwardsiella tarda, the causative agent of Edwardsiellosis, imposes medical challenges both in the clinic and aquaculture. The emergence of multidrug resistant strains makes antibiotic treatment impractical. The identification of molecules, which facilitate or promote antibiotic efficacy, are in high demand. In the present study, we aimed to identify small molecules whose abundance is correlated with kanamycin resistance in E. tarda by gas chromatography-mass spectrometry. We found that the abundance of fructose was greatly suppressed in kanamycin-resistant strains. The incubation of kanamycin-resistant bacteria with exogenous fructose sensitized the bacteria to kanamycin. Moreover, the fructose also functioned in bacteria persisters and biofilm. The synergistic effects of fructose and kanamycin were validated in a mouse model. Furthermore, the mechanism relies on fructose in activating TCA cycle to produce NADH, which generates proton motive force to increase the uptake of the antibiotics. Therefore, we present a novel approach in fighting against multidrug resistant bacteria through exploration of antibiotic-suppressed molecules.
    Journal of Proteome Research 02/2015; DOI:10.1021/pr501285f · 5.00 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Streptococcosis causes massive tilapia kills, which results in heavy economic losses of tilapia farming industry. Out of the Streptococcosis, Streptococcus agalactiae is the major pathogen. The bacterium causes higher mortality of tilapias in higher than lower temperatures. However, effect of temperature on metabolic regulation which is related to the mortality is largely unknown. The present study showed 50% and 70% mortality of tilapias cultured in 25 °C and 30 °C, respectively, in comparison with no death in 20 °C following infection caused by S. agalactiae. Then, GC/MS based metabolomics was used to investigate a global metabolic response of tilapia liver to the two higher water temperatures compared to 20 °C. Thirty-six and forty-five varied abundance of metabolites were identified in livers of tilapias cultured at 25 °C and 30 °C, respectively. More decreasing abundance of amino acids and increasing abundance of carbohydrates were detected in 30 °C than 25 °C groups. On the other hand, out of the pathways enriched, the first five biggest impact pathways belong to amino acid metabolism. Decreasing abundance of l-proline was identified as a crucial biomarker for indexing higher water temperature and a potential modulator to reduce the high death. This was validated by engineering injection or oral addition of l-proline. Exogenous l-proline led to elevated amino acid metabolism, which contributes to the elevated survivals. Our findings provide a potential metabolic modulator for controlling the disease, and shed some light on host metabolic prevention to infectious diseases. Copyright © 2015. Published by Elsevier Ltd.
    Fish &amp Shellfish Immunology 02/2015; 44(1). DOI:10.1016/j.fsi.2015.01.025 · 3.03 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Multidrug-resistant bacteria are an increasingly serious threat to human and animal health. However, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. Here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant Edwardsiella tarda by GC-MS-based metabolomics. Exogenous alanine or glucose restores susceptibility of multidrug-resistant E. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resistant bacteria. The mechanism underlying this approach is that exogenous glucose or alanine promotes the TCA cycle by substrate activation, which in turn increases production of NADH and proton motive force and stimulates uptake of antibiotic. Similar results are obtained with other Gram-negative bacteria (Vibrio parahaemolyticus, Klebsiella pneumoniae, Pseudomonas aeruginosa) and Gram-positive bacterium (Staphylococcus aureus), and the results are also reproduced in a mouse model for urinary tract infection. This study establishes a functional metabolomics-based strategy to manage infection by antibiotic-resistant bacteria. Copyright © 2015 Elsevier Inc. All rights reserved.
    Cell Metabolism 02/2015; 21(2):249-61. DOI:10.1016/j.cmet.2015.01.008 · 16.75 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Antibiotic-resistant Edwardsiella tarda poses a severe challenge to aquaculture. An understanding for antibiotic-resistant mechanisms is crucial to control the disease. The present study characterizes E. tarda ampicillin-stressed proteome and shows the importance of energy metabolism including the TCA cycle and glycolysis/gluconeogenesis in the antibiotic resistance. Further combination with antibiotic measurement develops a new method for identification of antibiotic-binding proteins out of differential abundances of proteins and results in determination of ETAE_0175 and ETAE_3367 as ampicillin-binding proteins in E. tarda. Genes of the two proteins are cloned and recombinant proteins are purified for validation of antibiotic-binding capability. Results show that higher binding capability is detected in ETAE_3367 than ETAE_0175. Out of the two proteins, ETAE_3367 is first reported here to be an antibiotic-binding protein, while ETAE_0175 homology in other bacteria has been shown to bind with other antibiotics. Bioinformatics analysis shows that ETAE_3367 may closely interact with aceF and sucA belonging to the TCA cycle and glycolysis/gluconeogenesis, respectively. These results indicate that energy metabolism contributes to ampicillin resistance in E. tarda and a new method to identify antibiotic-binding proteins is developed. These findings highlight the way to an understanding of antibiotic-resistant mechanisms in content of antibiotic-binding proteins.
    Journal of Proteomics 01/2015; 116. DOI:10.1016/j.jprot.2014.12.018 · 3.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose To evaluate the use of dual-energy spectral computed tomographic (CT) quantitative parameters compared with the use of conventional CT imaging features for preoperative diagnosis of metastasis to the cervical lymph nodes in patients with papillary thyroid cancer. Materials and Methods This study was approved by the ethics committee and all patients provided written informed consent. Analyses of quantitative gemstone spectral imaging data and qualitative conventional CT imaging features were independently performed by different groups of radiologists. Excised lymph nodes were located and labeled during surgery according to location on preoperative CT images and were evaluated histopathologically. Single and combined parameters were fitted to simple and multiple logistic regression models, respectively, by means of the generalized estimating equations method. Sensitivity and specificity analyses were performed by using receiver operating characteristic curves and were compared with data from the qualitative analysis. Results The slope of the spectral Hounsfield unit curve (λHU), normalized iodine concentration, and normalized effective atomic number (Zeff-c) measured during both arterial and venous phases were significantly higher in metastatic than in benign lymph nodes. The best single parameter for detection of metastatic lymph nodes was venous phase λHU, with sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of 62.0%, 91.1%, 80.6%, 79.7%, and 81.0%, respectively. The best combination of parameters was venous phase λHU and arterial phase normalized iodine concentration, with values of 73.0%, 88.4%, 82.9%, 78.0%, and 85.3%, respectively. Compared with qualitative analysis, the venous phase λHU showed higher specificity (91.1% vs 83.0%, P < .001) and similar sensitivity (62.0% vs 61.9%, P > .99), and the combined venous phase λHU and arterial phase normalized iodine concentration showed higher sensitivity (73.0% vs 61.9%, P < .001) and specificity (88.4% vs 83.0%, P < .001). Conclusion Quantitative assessment with gemstone spectral imaging quantitative parameters showed higher accuracy than qualitative assessment of conventional CT imaging features for preoperative diagnosis of metastatic cervical lymph nodes in patients with papillary thyroid cancer. © RSNA, 2014 Online supplemental material is available for this article.
    Radiology 12/2014; DOI:10.1148/radiol.14140481 · 6.21 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To evaluate the diagnostic value of diffusion-weighted magnetic resonance imaging for differentiating metastatic from non-metastatic retropharyngeal lymph nodes (RLNs) in patients with nasopharyngeal carcinoma (NPC). Materials and Methods: Untreated NPC patients (n=145) were scanned with both morphological magnetic resonance imaging (MRI) and diffusion-weighted imaging (DWI). RLNs (n=335) were classified as metastatic on the basis of response to therapy as assessed on follow up MRI. Morphological (short-and long-axial diameters) and functional (mean apparent diffusion coefficient (ADC) and minimum ADC values) parameters of the RLNs were derived from DWI and compared between metastatic and non-metastatic groups. A receiver operating characteristic (ROC) curve and the area under the curve (AUC) were used to evaluate the effectiveness of individual criteria and to generate threshold values to diagnose RLN metastases. Results: Statistically significant differences between metastatic and non-metastatic RLNs were found for all four parameters derived from DWI (p<0.001).At threshold values, accuracies of the ADC-based criteria (0.938 and 0.965 for mean and minimum ADC values) were greater than for size-based criteria (0.838 and 0.809 for short-and long-axial diameters). The minimum ADC value at the threshold of 0.89×10(-3)mm(2)/s was the most effective of all parameters in differentiating metastatic from non-metastatic RLNs with the sensitivity of95.7%, specificity of 95.1%, and accuracy of 96.5%. Conclusions: DWI is feasible for differentiating metastatic RLNs from non-metastatic nodes in NPC patients with high accuracy, and the minimum ADC derived from DWI could serve as a standard clinical marker for disease status.
    Dento-maxillo-facial radiology. Supplement 11/2014; DOI:10.1259/dmfr.20140126
  • [Show abstract] [Hide abstract]
    ABSTRACT: Optical sensor arrays typically require a large set of chemically responsive colorants to enhance discrimination capability. Conversely, we have proven that by using multiple concentrations of one indicator, the discrimination of various analytes could be realized. We have developed a simple array sensor, including only one indicator with varying concentrations, and successfully applied the array to discriminate eight anions at their wastewater discharge standard concentrations. The same idea has also been extended to the identification of seven heavy-metal ions at same concentrations. Chemometric analysis revealed that the sensor array has the capability to discriminate various analytes even it contained only one indicator with multiple concentrations.
    Chemical Communications 10/2014; DOI:10.1039/C4CC07503J · 6.72 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Streptococcus iniae infection has emerged as a serious fish health and economic problem in the global aquaculture operations. Current antibiotic options are few and possess severe practical limitations and potential adverse environmental impacts. The major factor contributing to the large burden of S. iniae disease in aquaculture is the lack of fundamental knowledge of innate immunity against the pathogen. In the present study, we use a tilapia model to explore which metabolites are crucial for the defense against the infection caused by S. iniae. We establish GC/MS based metabolic profile of tilapia liver and then compare the metabolic difference between survivals and the dying fish post the bacterial infection. We identify elevating N-acetylglucosamine in survival group as the most crucial metabolite differentiating the survivals from the dying in these fish infected by S. iniae. Exogenous N-acetylglucosamine significantly elevates survival ability of tilapia against the infection caused by S. iniae. Our findings highlight the importance of metabolic strategy against bacterial infections.
    Fish &amp Shellfish Immunology 10/2014; 40(2). DOI:10.1016/j.fsi.2014.08.008 · 3.03 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objectives Whether clopidogrel should be added to aspirin for stroke prevention remained controversial for the risk of hemorrhagic complications. This meta-analysis was aimed to assess the efficacy and safety of adding clopidogrel to aspirin on stroke prevention in high vascular risk patients, and to provide evidence for a suitable duration of dual antiplatelet therapy. Methods We searched PubMed, EMBase, OVID and Cochrane Central Register of Controlled Trials (up to June, 2013) for randomized controlled trials evaluating the efficacy and safety of clopidogrel plus aspirin versus aspirin alone in high vascular risk patients. Comparisons of stroke and hemorrhagic complications between treatment groups were expressed by the pooled Relative Risks (RRs) with 95% Confidence Intervals (CIs). Results Fifteen trials with a total of 97692 intention-to-treat participants were included with duration of follow-up ranging from 7 days to 3.6 years. Dual antiplatelet therapy reduced all stroke by 21% (RR: 0.79, 95% CI: 0.73–0.85) with no evidence of heterogeneity across the trials (P = 0.27, I2 = 17%).The effects were consistent between short-term subgroup (≤1 month, RR: 0.76, 95% CI: 0.67–0.85) and long-term subgroup (≥3 months, RR: 0.81, 95% CI: 0.73–0.89). The risk of major bleeding was not significantly increased by dual antiplatelet therapy in short-term subgroup (RR: 1.11, 95% CI: 0.91–1.36), while significantly increased in long-term subgroup (RR: 1.52, 95% CI: 1.36–1.69). Long-term dual antiplatelet therapy substantially increased the risk of intracranial bleeding (RR: 1.76, 95% CI: 1.22–2.54). Conclusions This meta-analysis demonstrates that short-term combination of clopidogrel and aspirin is effective and safe for stroke prevention in high vascular risk patients. Long-term combination therapy substantially increases the risk of major bleeding and intracranial bleeding.
    PLoS ONE 08/2014; 9(8):e104402. DOI:10.1371/journal.pone.0104402 · 3.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The parapharyngeal space (PPS) is an inverted pyramid-shaped deep space in the head and neck region, and a variety of tumors, such as salivary gland tumors, neurogenic tumors, nasopharyngeal carcinomas with parapharyngeal invasion, and lymphomas, can be found in this space. The differential diagnosis of PPS tumors remains challenging for radiologists. The aim of this study was to develop and test a modified method for locating PPS tumors on magnetic resonance (MR) images to improve preoperative differential diagnosis. The new protocol divided the PPS into three compartments: a prestyloid compartment, the carotid sheath, and the areas outside the carotid sheath. PPS tumors were located in these compartments according to the displacements of the tensor veli palatini muscle and the styloid process, with or without blood vessel separations and medial pterygoid invasion. This protocol, as well as a more conventional protocol that is based on displacements of the internal carotid artery (ICA), was used to assess MR images captured from a series of 58 PPS tumors. The consequent distributions of PPS tumor locations determined by both methods were compared. Of all 58 tumors, our new method determined that 57 could be assigned to precise PPS compartments. Nearly all (13/14; 93%) tumors that were located in the pre-styloid compartment were salivary gland tumors. All 15 tumors within the carotid sheath were neurogenic tumors. The vast majority (18/20; 90%) of trans-spatial lesions were malignancies. However, according to the ICA-based method, 28 tumors were located in the pre-styloid compartment, and 24 located in the post-styloid compartment, leaving 6 tumors that were difficult to locate. Lesions located in both the pre-styloid and the post-styloid compartments comprised various types of tumors. Compared with the conventional ICA-based method, our new method can help radiologists to narrow the differential diagnosis of PPS tumors to specific compartments.
    Ai zheng = Aizheng = Chinese journal of cancer 08/2014; DOI:10.5732/cjc.014.10017
  • [Show abstract] [Hide abstract]
    ABSTRACT: Research on the migration and transformation of phenol with space and temporal variability in the vadose zone is hindered by monitoring technology in field experiments. Four column experiments were conducted to investigate the effect of porous media size, volatilization, biological effects, and oxidation on the transport of phenol in the vadose zone. The initial inflow phenol concentration of each column was 500 mg/L, and the final outflow concentrations were 0, 348, 240, and 365 mg/L, More than 90% of reduction of phenol concentration occurred at the top of the simulation column. Results show that volatilization and oxidation are the main factors that could lead to the decrease of phenol concentration in an open system. However, these two processes cannot be accurately separated. The migration rate of phenol was larger in coarse sands (6.06 cm/d) than in fine sands (4.55 cm/d). Phenol biodegradation did not occur under experimental conditions. However, mercury (as biological inhibitor) could react with phenol to generate a mercury-phenol complex, which could lead to the reduction of phenol concentration to 21.6% in the simulation experiment.
    Water Science & Technology 08/2014; 70(4):685-690. DOI:10.2166/wst.2014.279 · 1.21 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The sensitive determination of heavy-metal ions has been widely investigated in recent years due to their threat to the environment and to human health. Among various analytical detection techniques, inexpensive colorimetric testing papers/strips play a very important role. The limitation, however, is also clear: the sensitivity is usually low and the selectivity is poor. In this work, we have developed a postage stamp-sized array sensor composed of nine commercially available heterocyclic azo indicators. Combining filtration-based enrichment with an array of technologies-based pattern-recognition, we have obtained the discrimination capability for seven heavy-metal ions (Hg(2+), Pb(2+), Ag(+), Ni(2+), Cu(2+), Zn(2+), and Co(2+)) at their Chinese wastewater discharge standard concentrations. The allowable detection level of Hg(2+) was down to 0.05 mg L(-1). The heavy-metal ions screening test was readily achieved using a standard chemometric approach. And the array sensor applied well in real water samples.
    The Analyst 07/2014; 139(19). DOI:10.1039/c4an01022a · 3.91 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Interferon (IFN) family is a large group of cytokines involved in innate immune response against various microorganisms. However, whether IFN functions in antimicrobial property by metabolic pathways is largely unknown. In the present study, GC/MS-based metabolome is investigated in humoral fluid of zebrafish (Dario rerio) which are exposed to three doses of IFN-α2b, designed as IFN-L, IFN-M and IFN-H. Out of 67 compounds identified, 19, 28 and 29 differential abundances of metabolites are identified in the three groups compared with control, respectively. Total 43 differential metabolites constructed IFN-dependent metabolome, in which thirteen overlap among the three doses of IFN-α2b groups. These overlapped metabolites show that decreased alanine asparate and glutamate metabolic pathway, arginine and proline metabolic pathway and increased purine metabolism form a characteristic feature in response to IFN-α2b. Further dose-related metabolites indicate that biosynthesis of unsaturated fatty acids is enriched only in IFN-M and IFN-H, which is related to high protection against bacterial infection. Exogenous fatty acids, especially unsaturated linoleic acid, may elevate survival ability of zebrafish infected with extracellular pathogenic V. alginolyticus and intracellular pathogenic Edwardsiella tarda. These results disclose an unknown mechanism by which IFN-α2b protects host from microbial infections. Our findings highlight the ways to understand action of IFN in content of metabolic regulation.
    Journal of Proteome Research 07/2014; 13(9). DOI:10.1021/pr500592x · 5.00 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The control of IL-10 production in Toll-like receptor (TLR) signals remains to be elucidated. Here, we report that β-arrestin 2 positively regulates TLR-triggered IL-10 production in a p38 mitogen-activated protein kinase (MAPK)-dependent mechanism. In vitro studies with cells including peritoneal macrophages and HEK293/TLR4 cells have demonstrated that β-arrestin 2 forms complexes with p38 and facilitates p38 activation after lipopolysaccharide (LPS) stimulation. Deficiency of β-arrestin 2 and inhibition of p38 MAPK activity both ameliorate TLR4-stimulated IL-10 response. Additionally, in vivo experiments show that mice lacking β-arrestin 2 produce less amount of IL-10, and are more susceptible to LPS-induced septic shock which is further enhanced by blocking IL-10 signal. These results reveal a novel mechanism by which β-arrestin 2 negatively regulates TLR4-mediated inflammatory reactions.
    Journal of Biological Chemistry 07/2014; 289(33). DOI:10.1074/jbc.M114.591495 · 4.60 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The high-pressure behaviors of SmFeO3 are investigated by angle-dispersive synchrotron X-ray powder diffraction under a pressure of up to 40.3 GPa at room temperature. The crystal structure of SmFeO3 remains stable at up to the highest pressure. The different pressure coefficients of the normalized axial compressibility are obtained to be beta(a) = 0.60 x 10(-3) GPa(-1), beta(b) = 0.79 x 10(-3) GPa(-1), beta(c) = 1.28 x 10(-3) GPa(-1), and the bulk modulus (B-0) is determined to be 293(3) GPa by fitting the pressure-volume data using the Birch-Murnaghan equation of state. Furthermore, the larger compressibility of the FeO6 octahedra suggests the evolution of the orthorhombic structure towards higher symmetry configuration at high pressures.
    Chinese Physics B 06/2014; 23(6):069101. DOI:10.1088/1674-1056/23/6/069101 · 1.39 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Hypertension is common after acute stroke onset. Previous studies showed controversial effects of early blood pressure (BP) lowering on stroke outcomes. The aim of this study is to assess the effects of early BP lowering on early and long-term outcomes after acute stroke. Methods A meta-analysis was conducted with prospective randomized controlled trials assessing the effects of early BP lowering on early and long-term outcomes after acute stroke compared with placebo. Literature searching was performed in the databases from inception to December 2013. New evidence from recent trials were included. Outcomes were analyzed as early (within 30 days) and long-term (from 3 to 12 months) endpoints using summary estimates of relative risks (RR) and their 95% confidence intervals (CI) with the fixed-effect model or random-effect model. Results Seventeen trials providing data from 13236 patients were included. Pooled results showed that early BP lowering after acute stroke onset was associated with more death within 30 days compared with placebo (RR: 1.34 and 95% CI: 1.02, 1.74, p = 0.03). However the results showed that early BP lowering had no evident effect on early neurological deterioration, early death within 7 days, long-term death, early and long-term dependency, early and long-term combination of death or dependency, long-term stroke recurrence, long-term myocardial infarction and long-term CVE. Conclusions The new results lend no support to early BP lowering after acute stroke. Early BP lowering may increase death within 30 days after acute stroke.
    PLoS ONE 05/2014; 9(5):e97917. DOI:10.1371/journal.pone.0097917 · 3.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Microbial disease problems constitute the largest single cause of economic losses in aquaculture. An understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. In the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by Edwardsiella tarda EIB202. We establish the metabolic profile of crucian carps and then compare the metabolic difference between survivals and dead fish by self-control. We identify elevating unsaturated fatty acid biosynthesis and decreasing fructose and mannose metabolism as the most key pathways and increasing palmitic acid and decreasing D-mannose as the most crucial metabolites differentiating survivals from death in these fish infected by E. tarda. Our findings highlight the importance of metabolic strategy against bacterial infections.
    Fish &amp Shellfish Immunology 05/2014; DOI:10.1016/j.fsi.2014.04.017 · 3.03 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The structural and electrical properties of ZnV2O6 under high pressure have been studied using Raman spectroscopy, in situ angle dispersive X-ray diffraction (ADXRD), and alternating current (AC) impedance spectroscopy. The results of Raman spectra indicate that ZnV2O6 undergoes a reversible structural change around 16.6 GPa, as evidenced by the appearance of new peaks. The results of Rietveld refinements from in situ ADXRD data indicate that the monoclinic symmetry (C2/m) is retained up to 16.0 GPa and the C2 phase comes to coexist between 16.0 and 16.9 GPa. Above 16.9 GPa, the high-pressure phase can be distinguished only as the C2 structure. The transformation process from the C2/m phase to the C2 phase is mainly caused by the more distorted ZnO6 octahedra and VO6 octahedra at higher pressures. The equal bond distances Zn–O2 and V–O3 in the C2/m phase become unequal in the C2 phase. Furthermore, the measurements of the AC impedance spectroscopy of ZnV2O6 reveal obvious changes in its electrical transport properties at 14.1 GPa which could correspond to the observed phase transition in the Raman and ADXRD measurements. The combined analyses of experimental results suggest the occurrence of a reversible structural phase transition of ZnV2O6 around 16.0 GPa.
    The Journal of Physical Chemistry C 05/2014; 118(20):10560–10566. DOI:10.1021/jp411283m · 4.84 Impact Factor

Publication Stats

719 Citations
382.42 Total Impact Points

Institutions

  • 2008–2015
    • Sun Yat-Sen University
      • • School of Life Sciences
      • • State Key Laboratory of Biocontrol
      • • Department of Orthopaedic Surgery
      Shengcheng, Guangdong, China
  • 2014
    • Guangdong Academy of Medical Sciences and General Hospital
      Shengcheng, Guangdong, China
  • 2012–2014
    • Dalian Institute of Chemical Physics
      Lü-ta-shih, Liaoning, China
    • Jilin University
      • State Key Lab of Superhard Materials
      Yung-chi, Jilin Sheng, China
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China
  • 2011–2014
    • Beijing University of Aeronautics and Astronautics (Beihang University)
      • School of Instrumentation Science and Opto-electronics Engineering (SISOE)
      Peping, Beijing, China
  • 2009–2014
    • East Tennessee State University
      • Department of Internal Medicine
      Johnson City, Tennessee, United States
  • 2013
    • Nanjing University
      Nan-ching, Jiangsu Sheng, China
    • University of Texas Southwestern Medical Center
      • Division of Neuro-oncology
      Dallas, Texas, United States
    • Huazhong Agricultural University
      • College of Resources and Environment
      Wu-han-shih, Hubei, China
  • 2011–2013
    • Vanderbilt University
      • • Department of Medicine
      • • Division of Allergy, Pulmonary and Critical Care
      Nashville, MI, United States
  • 2009–2012
    • Huazhong University of Science and Technology
      • Department of Nuclear Medicine
      Wu-han-shih, Hubei, China
  • 2010
    • Sun Yat-Sen University Cancer Center
      Shengcheng, Guangdong, China
  • 2007–2010
    • Zhongshan University
      Shengcheng, Guangdong, China
    • Xiamen University
      • Department of Biology
      Xiamen, Fujian, China