Hongchen Liu

Chinese PLA General Hospital (301 Hospital), Peping, Beijing, China

Are you Hongchen Liu?

Claim your profile

Publications (47)98 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Long non-coding RNAs (lncRNAs) have recently attracted more attention about the role in a broad range of biological processes and complex cancers. We aimed to identify differentially expressed lncRNAs that play an important role in the pathogenesis of oral squamous cell carcinoma (OSCC). Microarray data GSE25099 consisting of 57 samples from patients with OSCC and 22 normal samples were downloaded from Gene Expression Omnibus database. Differentially expressed genes (DEGs) and lncRNAs were identified between OSCC samples and control using samr package in R and noncoder software. Co-expression network was constructed for lncRNAs and candidate target DEGs, followed by functional and pathway enrichment analysis using the Database for Annotation, Visualization and Integrated Discovery online tool. OSCC-related genes were screened by Genetic-Association-DB-Database analysis, and then protein-protein interaction (PPI) network construction of OSCC-related and co-expressed genes. Bioinformatic analysis revealed that there were 998 DEGs and 160 differentially expressed lncRNAs between OSCC and normal control. We found LOC100130547, FTH1P3, PDIA3F and GTF2IRD2P1 targeted most of DEGs. Predicted targets-related functional annotation showed significant changes in inflammation-related functions and Toll-like receptor signaling pathway. By further conducting PPI network with lncRNA co-expressed DEGs, we found that OSCC-associated genes including MMP1 (matrix metallopeptidase), MMP3, MMP9, PLAU (plasminogen activator, urokinase) and IL8 (interleukin 8) were targeted by FTH1P3, PDIA3F and GTF2IRD2P1. Our results indicate that lncRNAs FTH1P3, PDIA3F and GTF2IRD2P1 may responsible for progression and metastasis of OSCC via targeting MMP1, MMP3, MMP9, PLAU and IL8 which are key regulators of tumorigenesis. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Archives of oral biology 08/2015; 60(10):1581-1587. DOI:10.1016/j.archoralbio.2015.08.003 · 1.88 Impact Factor
  • Shanchuan Zhang · Xiaowei Shi · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: To analyze angulations of anterior teeth with reference to the alveolar bone. Cone-beam computed tomographic (CBCT) images of 105 participants were taken with the same machine (ProMax 3D Max CBCT), showing the intact anterior teeth. The angulations formed between the long axis of the anterior teeth and the alveolus were measured using cross-sectional images. The thicknesses of alveolar bone on different area of root surfaces were also measured. Maxillary anterior teeth were found to be close to the buccal alveolar surface in apical level with the angulations referred to alveolar bone in center incisor, lateral incisor, and canine were 17.65 ± 6.8, 18.79 ± 7.4, and 23.82 ± 6.96 degrees, respectively. Means of angulations of mandibular anterior teeth were less than 8 degree. The thicknesses of buccal bone at mid-root level in 77% to 90% maxillary anterior teeth were less than 1 mm. The determinations provided high intrarater/interrater reliability. The application of CBCT is reliable for a complete calculation of angulations and thicknesses in a Chinese Han population. As a result, adequate planning combined with image examination preoperatively would contribute to a favorable outcome.
    Implant dentistry 06/2015; DOI:10.1097/ID.0000000000000291 · 1.11 Impact Factor
  • Zhihui Wang · Yanyi Wang · Hongchen Liu · Yuwei Che · Yingying Xu · Lingling E
    [Show abstract] [Hide abstract]
    ABSTRACT: Free radical hypothesis which is one of the most acknowledged aging theories was developed into oxidative stress hypothesis. Protein carbonylation is by far one of the most widely used markers of protein oxidation. We studied the role of age and gender in protein carbonyl content of saliva and plasma among 273 Chinese healthy subjects (137 females and 136 males aged between 20 and 79) and discussed the correlation between protein carbonyl content of saliva and plasma. Protein carbonyl content of saliva and plasma were, respectively, 2.391 ± 0.639 and 0.838 ± 0.274 nmol/mg. Variations of saliva and plasma different age groups all reached significant differences in both male and female (all p < 0.05) while both saliva and plasma protein carbonyls were found to be significantly correlated with age (r = 0.6582 and r = 0.5176, all p < 0.001). Gender was discovered to be unrelated to saliva and plasma protein carbonyl levels (all p > 0.05). Saliva and plasma protein carbonyls were positively related (r = 0.4405, p < 0.001). Surprisingly, saliva and plasma protein carbonyls/ferric reducing ability of plasma (FRAP) ratios were proved to be significantly correlated with age (r = 0.7796 and r = 0.6938, all p < 0.001) while saliva protein carbonyls/FRAP ratio and plasma protein carbonyls/FRAP ratio were also correlated (r = 0.5573, p < 0.001). We concluded that saliva protein carbonyls seem to be an alternative biomarker of aging while the mechanisms of protein carbonylation and oxidative stress and the relationship between saliva protein carbonyls and diseases need to be further investigated.
    Age 06/2015; 37(3):9781. DOI:10.1007/s11357-015-9781-1 · 3.45 Impact Factor
  • Bo Zhang · Na Liu · Haigang Shi · Hao Wu · Yuxuan Gao · Huixia He · Bin Gu · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Diabetes mellitus involves metabolic changes that can impair bone repair. Bone mesenchymal stem cells (BMSCs) play an important role in bone regeneration. However, the bone regeneration ability of BMSCs is inhibited in high glucose microenvironments. It can be speculated that this effect is due to changes in BMSCs' proliferation and migration ability, because the recruitment of factors with an adequate number of MSCs and the microenvironment around the site of bone injury are required for effective bone repair. Recent genetic evidence has shown that the Cyclin D1 and the CXC receptor 4 (CXCR-4) play important roles in the proliferation and migration of BMSCs. In this study we determined the specific role of glycogen synthase kinase-3β (GSK3β) in the proliferation and migration of BMSCs in high glucose microenvironments. The proliferation and migration ability of BMSCs were suppressed under high glucose conditions. We showed that high glucose activates GSK3β but suppresses CXCR-4, β-catenin, LEF-1, and cyclin D1. Inhibition of GSK3β by LiCl led to increased levels of β-catenin, LEF-1, cyclin D1, and CXCR-4 expression. Our data indicate that GSK3β plays an important role in regulating the proliferation and migration of BMSCs by inhibiting cyclin D1 and CXCR-4 under high glucose conditions.
    Journal of Bone and Mineral Metabolism 04/2015; DOI:10.1007/s00774-015-0662-6 · 2.11 Impact Factor
  • Xue Han · Xia Wu · Hongchen Liu · Dongsheng Wang · Lingling E · Wei Zhou
    [Show abstract] [Hide abstract]
    ABSTRACT: An injectable bone cement, nHAC/CSH, which consists of nano-hydroxyapatite/collagen (nHAC) and calcium sulphate hemihydrate (CaSO4.½H2O; CSH) was investigated as a tissue-engineered scaffold material with blood-acquired mesenchymal progenitor cells (BMPCs) as seeding cells. An in vitro study on the cytocompatability of nHAC/CSH and an in vivo study on the ectopic bone formation of nHAC/CSH loaded with dBMPCs were both conducted. The dBMPCs morphology, proliferation, differentiation and apoptosis assays were conducted using the direct contact and extract method. The cells tests exhibited normal growth and bioactive function in vitro. Studies in vivo showed that this injectable tissue engineered bone (ITB) formed bone structure in the heterotopic site of nude mice. These findings indicate that the ITB composed of nHAC/CSH and dBMPCs may represent a useful strategy for clinical reconstruction of irregular bone defects.
    Journal of Materials Science Materials in Medicine 01/2015; 26(1):5338. DOI:10.1007/s10856-014-5338-6 · 2.38 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective. A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here. Methods. Composition, setting time, injectability, compressive strength, and bioactivity in simulated body fluid (SBF) of the Mg/CSH composite were evaluated. Furthermore, the cellular responses of canine bone marrow stromal cells (cBMSCs) and bone formation capacity after the implantation of Mg/CSH in tibia defects of canine were investigated. Results. Mg/CSH possessed a prolonged setting time and markedly improved injectability and mechanical property . Mg/CSH samples showed better degradability than CSH in SBF after 21 days of soaking . Moreover, the degrees of cell attachment, proliferation, and capability of osteogenic differentiation on the Mg/CSH specimens were higher than those on CSH, without significant cytotoxicity and with the increased proliferation index, ALP activity, and expression levels of integrin β1 and Coll I in cBMSCs . Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level . Conclusions. The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.
    01/2015; 2015:1-15. DOI:10.1155/2015/297437
  • Source
    Pan Ma · Bin Gu · Wei Xiong · Baosheng Tan · Wei Geng · Jun Li · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Our previous studies demonstrated that glimepiride enhanced the proliferation and differentiation of osteoblasts and led to activation of the PI3K/Akt pathway. Recent genetic evidence shows that endothelial nitric oxide synthase (eNOS) plays an important role in bone homeostasis. In this study, we further elucidated the roles of eNOS, PI3K and Akt in bone formation by osteoblasts induced by glimepiride in a high glucose microenvironment. We demonstrated that high glucose (16.5 mM) inhibits the osteogenic differentiation potential and proliferation of rat osteoblasts. Glimepiride activated eNOS expression in rat osteoblasts cultured with two different concentrations of glucose. High glucose-induced osteogenic differentiation was significantly enhanced by glimepiride. Down-regulation of PI3K P85 levels by treatment with LY294002 (a PI3K inhibitor) led to suppression of P-eNOS and P-AKT expression levels, which in turn resulted in inhibition of RUNX2, OCN and ALP mRNA expression in osteoblasts induced by glimepiride at both glucose concentrations. ALP activity was partially inhibited by 10 µM LY294002. Taken together, our results demonstrate that glimepiride-induced osteogenic differentiation of osteoblasts occurs via eNOS activation and is dependent on the PI3K/Akt signaling pathway in a high glucose microenvironment.
    PLoS ONE 11/2014; 9(11):e112243. DOI:10.1371/journal.pone.0112243 · 3.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Zinc finger protein, X-linked (ZFX) has been identified as a transcriptional factor and is implicated in the development of variant types of cancer. Furthermore, it has been reported that ZFX is essential for the survival and self-renewal of embryonic stem cells. To investigate the involvement of ZFX in squamous cell carcinoma of the tongue, in the present study, we explored the expression of ZFX in clinical specimens from patients with squamous cell carcinoma of the tongue and the correlation between ZFX expression and multiple clinical pathological parameters. We further evaluated the impact of ZFX knockdown on the proliferation, colony formation ability, cell cycle distribution and survival of two human tongue squamous cell carcinoma cell lines to explore its critical role in the development of squamous cell carcinoma of the tongue. Our results showed that ZFX expression was aberrantly higher in samples from patients with squamous cell carcinoma of the tongue and revealed that ZFX expression is positively correlated with tumor grade and stage. Consistent with these findings, we further found that ZFX knockdown impaired cell proliferation and colony formation ability and induced cell apoptosis and cell cycle arrest in two human tongue squamous cell carcinoma cell lines. Our results indicate that ZFX is essential for the development and progression of squamous cell carcinoma of the tongue and represents a potential target for the development of effective therapy.
    Oncology Reports 10/2014; 33(1). DOI:10.3892/or.2014.3572 · 2.19 Impact Factor
  • Lin Feng · E Lingling · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the effects of autologous blood vessels and nerves on vascularization.
    Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 10/2014; DOI:10.5507/bp.2014.050 · 1.66 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san (DSS) is a traditional Chinese medicine (TCM) prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia.
  • Bin Gu · Na Liu · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: To observe the activity change of astrocyte in related nucleus caused by acute pulpitis in rats.
  • Pan Ma · Baosheng Tan · Hongchen Liu · Junli Ma · Bin Gu
    [Show abstract] [Hide abstract]
    ABSTRACT: To explore the effect of glimepiride on the glucose uptake as well as glucose transporter (GLUT)-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia.
  • Hong Guo · Yu Gao · Bin Gu · Jing Wang · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective To investigate the antiosteoporotic effects of L-Arginine on ovariectomied rats. Methods Forty twelve-week-old female Sprague-Dawley rats were randomly divided into four groups of bilaterally ovariectomy and one group of Sham animals, with 8 animals in each group. Twelve additional weeks elapsed before initiation of the treatment with L-Arginine in order to induce significant bone loss in the ovariectomied animals. A 4-week daily treatment (5 days a week, Monday–Friday) at doses of 5 mg/kg/d, 10 mg/kg/d, 20 mg/kg/d of L-Arginine or vehicle only was administered by s.c. injection to the ovariectomied groups respectively. At the end of the treatment period, blood samples from all animals were collected for biochemical analysis. Micro-computerized tomography analysis, histopathological study and biomechanical test were performed on the femur of each animal. Results Serum alkaline phosphatase and osteocalcin were reduced in ovariectomied rats after the administration of different dosage of L-Arginine. L-Arginine of 10 mg/kg showed the most significant effect. Micro-computerized tomography 3-D images revealed that the increase of bone mass in 5 mg/kg and 10 mg/kg groups were significant greater than that in Sham group. The biomechanical parameters of femur were improved significantly in L-Arginine 10 mg/kg group when compared to untreated ovariectomied rats. Conclusions L-Arginine (10 mg/kg) contributes significantly to the treatment of the bone loss induced by ovariectomy on rats, demonstrated by increased bone mass, improved bone structure and recovery of bone biomechanical activity.
    Biomedicine and Aging Pathology 04/2014; 4(2). DOI:10.1016/j.biomag.2014.02.002
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To study the relationship between vascular endothelial growth factor (VEGF) and formation and repair of engineering bone, second-generation bone marrow stromal cells (BMSCs) of New Zealand white rabbits that were separated in vitro were transfected with VEGF 165 gene vectors by adenovirus to detect gene expressions. Transfected BMSCs and β-tricalcium phosphate material were complexed and implanted at the femoral injury sites of the study group (n = 12), and the control group (n = 12) were implanted with engineering bones that were not transfected with VEGF. Femoral recoveries of the two groups were observed on the 15th, 30th, 45th and 60th days, and their vascularization and ossification statuses were observed by immunohistochemical methods. The BMSCs transfected with VEGF highly expressed VEGF genes and excreted VEGF. The two groups both experienced increased vascularization and bone volume after implantation (t = 7.92, P<0.05), and the increases of the study group were significantly higher than those of the control group (t = 6.92, P<0.05). VEGF is clinically applicable because it can accelerate the formation and repair of engineering bone by promoting vascularization and ossification.
    PLoS ONE 12/2013; 8(12):e82945. DOI:10.1371/journal.pone.0082945 · 3.23 Impact Factor
  • Lin Wang · Xia Xu · Na Huo · Huiling Guo · Dongshen Wang · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Osteocyte generation can be used in bone defect repair; the generation efficiency needs to be further improved. This study aims to evaluate the role of ubiquitin A20 (A20) in facilitating the expression of osteocalcin in adipose-derived stem cells (ADSCs). In this study, adipose tissue was obtained from 10 healthy human subjects; ADSCs were isolated from the adipose samples. The ADSCs were transfected with core binding factor alpha 1 (Cbfa1) and/or insulin-like growth factor-1 receptor (IGF-1R). Expression of osteocalcin, A20 in ADSCs was assessed by quantitative RT-PCR (qRT-PCR) and Western blotting. Apoptosis of ADSCs was analyzed by flow cytometry. The results showed that after the gene transfection and stimulation of insulin, the ADSCs expressed high levels of osteocalcin. However, apoptotic ADSCs were induced by the activation of IGF-1R. Exposure to insulin down-regulated the expression of Bcl-xL and A20, and increased Bax, in ADSCs. The addition of exogenous A20 prevented the ADSC apoptosis. We conclude that activation of IGF-1R can induce apoptosis in ADSCs, which can be prevented by addition of exogenous A20.
    Biochemistry and Cell Biology 12/2013; 91(6):513-8. DOI:10.1139/bcb-2013-0043 · 2.35 Impact Factor
  • Yihan Liu · Ming Jiang · Wei Hao · Wenjia Liu · Liang Tang · Hongchen Liu · Yan Jin
    [Show abstract] [Hide abstract]
    ABSTRACT: The disappearance of ameloblasts in erupted teeth hampers the implementation of tissue engineering-based tooth regeneration. We aimed at utilizing skin epithelial cells as the appropriate substitute for ameloblasts. The conversion potential of 1 day postnatal rat skin epithelial cells to ameloblasts was investigated under the induction of dental papillae mesenchymal cells (DPMCs). Induction strategies had been designed both in vitro and in vivo. Markers for ameloblasts had been detected in skin epithelial cells, which showed a columnar appearance with the nuclei located at one side, under indirect co-culture with DPMCs in vitro. An enamel-dentine-like and tooth germ-like structure was formed by recombining skin epithelial pieces or cells with DPMCs after 14 days of implantation in rat renal capsule. Immunohistochemistry and cell labelling analysis further demonstrated that the enamel-forming cells were skin epithelium-derived. These results indicated that the skin epithelium-derived cells from postnatal rats have the potential to convert to functional ameloblasts under effective induction. Copyright © 2012 John Wiley & Sons, Ltd.
    Journal of Tissue Engineering and Regenerative Medicine 12/2013; 7(12). DOI:10.1002/term.1485 · 4.43 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Reconstruction of large area bone defect with mechanical integrity to the skeleton is important for patient's rehabilitation. However with the limitation of scaffold material and suitable seed cell sources, the best treating strategy remains to be identified though various tissue engineering methods were reported. In this study, we investigated the feasibility of applying calcined bovine bone (CBB) which was coated by allograft bone marrow mesenchymal stem cells (BMSC)-sheet as a 3D scaffold material in bone repairing tissue engineering. The new scaffold material was implanted into osteoporosis rat cranial bone defects and repairing critical size bone defects (8 mm diameter). Data showed that CBB-BMSC-sheet combination had a stronger potential in osteogenic differentiation and mineralized formation both in vitro and in vivo than CBB-BMSC combination. In in vitro study BMSC-sheet had a more feasible characteristic upon bone repairing including richer ECM, larger mineralized area and stronger ALP activity in addition with a significant higher mRNA expression of osteogenic maker such as BMP-2, b-FGF, Col 1a1, OSX and Runx-2 than the control group. In in vivo study 3D reconstruction of micro CT, HE staining and bone strength results showed that newly formed bone in CBB-BMSC-sheet group was significant higher than that in CBB-BMSC group at 4, 8 and 12 weeks after transplantation in the aspect of area and volume. What was more, results indicated that allograft BMSC-sheet had survivaled in the scaffold material and participated in the newly formed bone which had the same thickness with surrounding autologous bone tissues after transplantation. Results of our study demonstrated that CBB-BMSC-sheet combination was a promising strategy in healing of large area bone defect in osteoporosis.
    Biomaterials 09/2013; 34(38). DOI:10.1016/j.biomaterials.2013.09.040 · 8.31 Impact Factor
  • Lin Wang · Hongwei Wang · Xia Xu · Dongshen Wang · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Tissue engineering of bone has been increasingly used in the bone defect repair. To generate osteoblasts is a major approach, and here we have examined ways of improving the efficiency of producing osteoblasts. Adipose stem cells (ADSC) were prepared from rat mesentery tissue, and transfected with Cbfa1 gene vector or/and IGF-1R gene vector. The cells were stimulated with insulin. Osteocalcin expression by the ADSCs was assessed by quantitative RT-PCR (qRT-PCR), Western blotting and enzyme-linked immunobosorbent assay. Both genes Cbfa1 and IGF-1R were transfected in ADSCs, as shown by qRT-PCR and Western blotting. Stimulation by insulin in the culture increased osteocalcin expression in ADSCs transfected by both Cbfa1 and IGF-1R, but not in those transfected with only one of these two genes. Osteocalcin in the culture supernatant was also increased by stimulation with insulin. Thus IGF-1R gene transfer together with insulin stimulation can markedly increase the efficiency of generation of osteoblasts.
    Cell Biology International 07/2013; DOI:10.1002/cbin.10147 · 1.64 Impact Factor
  • Li Liang · Jifeng Yu · Wei Zhou · Na Liu · Lingling E · Dongsheng Wang · Hongchen Liu
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Endothelin-1(ET-1) is a 21-amino-acid peptide with multifunctional regulation. Initial research indicated that ET-1 is related to the inflammatory pathogenesis of periodontitis and involved in the regulation of cytokines, but the mechanisms involved remain unclear. The primary aim of this study was to investigate how ET-1 affects proinflammatory cytokine expression in human periodontal ligament (hPDL) cells. Methods: hPDL cells were obtained from both healthy (H-hPDL cells) and periodontitis-affected periodontal tissues (P-hPDL cells). The H-hPDL cells and P-hPDL cells were treated with ET-1 (1nM, 10nM, and 100nM) for 12, 24, and 48 hours(hrs). The untreated cells served as a normal control. To confirm the specificity of the ET-1 effects, we used 100nM of the specific endothelin A receptor(ETA) antagonist BQ123, and 100nM of the specific endothelin B receptor (ETB) antagonist BQ788 , as negative control. To examine the signaling pathways and molecular mechanisms involved in ET-1-mediated cytokine expression, The H-hPDL cells and P-hPDL cells were pre-treated with specific inhibitors for signal-regulated kinase (ERK1/2)(PD98059), c-Jun N-terminal kinase(JNK)(SP600125), and p38 kinase(SB203580) for 1 hrs before 100nM ET-1 stimulation. The tumour necrosis factor-alpha (TNF-α), interleukin(IL)-1β (IL-1β) and IL-6 mRNA and protein levels were evaluated by quantitative real-time PCR and ELISA, respectively. Results: ET-1 dose- and time- dependently induced the production of proinflammatory cytokines TNF-α, IL-1β, and IL-6 by H-hPDL cells and P-hPDL cells at both mRNA and protein levels. However, The ETA and ETB receptor antagonists inhibited the stimulatory effects of ET-1 on inflammatory cytokine expression in H-hPDL cells and P-hPDL cells. Furthermore, inhibitors of the Mitogen-activated protein kinases (MAPKs) significant reduced in ET-1-stimulated TNF-α, IL-1β, and IL-6 expression in H-hPDL cells and P-hPDL cells. Conclusion: ET-1 may be involved in the inflammatory process of periodontitis, at least in part, by stimulating proinflammatory cytokine production via MAPKs pathway in hPDL cells.
    Journal of Periodontology 05/2013; DOI:10.1902/jop.2013.130195 · 2.57 Impact Factor
  • Jiaqiang Liu · Yi Jiang · Jing Mao · Bin Gu · Hongchen Liu · Bing Fang
    [Show abstract] [Hide abstract]
    ABSTRACT: Periodontitis is one of the main complications of diabetes mellitus and many researches have been done on the relationship between periodontitis and diabetes mellitus, but too much are still unclear, especially the mechanisms by which high glucose induces damage of periodontal ligament fibroblasts. So in this study, we investigated the effects of different concentration of high glucose on apoptosis in human periodontal ligament fibroblasts and the possible mechanisms involved. Human periodontal ligament fibroblasts were cultured and subjected to glucose of different concentration (5.5, 15, 25, and 35 mM) for 24 h. Apoptosis was studied by flow cytometry, caspase assays, fluorescent real-time PCR and Western blot. We also determined Fas/FasL expression was by Western blot. The application of different concentration of high glucose induced a concentration-dependent increase of apoptosis and the activity of caspase-3 in cultured human periodontal ligament fibroblasts. Furthermore, inhibitor of caspase-3 could prevent the high-glucose-induced apoptosis in human periodontal ligament fibroblasts. Protein levels of Fas and FasL remained unchanged. These data indicate that high glucose induces a concentration- and caspase-3-dependent increase of apoptosis in cultured human periodontal ligament fibroblasts in vitro. Activation of caspase-3 caused by high glucose is independent of Fas/FasL signaling pathways system. These results suggest a novel mechanism for the regulation of human periodontal ligament fibroblasts apoptosis by high glucose.
    Applied biochemistry and biotechnology 05/2013; 170. DOI:10.1007/s12010-013-0287-y · 1.74 Impact Factor

Publication Stats

182 Citations
98.00 Total Impact Points

Institutions

  • 2008–2015
    • Chinese PLA General Hospital (301 Hospital)
      • Department of Orthopaedics
      Peping, Beijing, China
  • 2008–2013
    • 307 Hospital of the Chinese People's Liberation Army
      Peping, Beijing, China
  • 2010
    • Chengdu Military General Hospital
      Hua-yang, Sichuan, China