[Show abstract][Hide abstract] ABSTRACT: This study aimed to compare the regulatory T cells in cord blood of appropriate for gestational age (AGA) neonates with those of small for gestational age (SGA) neonates.
Umbilical cord blood was collected upon labor in 108 healthy full-term (between 37 and 41 gestational weeks) neonates, who were born between November 2010 and April 2012. Among them, 77 samples were obtained from AGA neonates, and 31 samples were obtained from SGA neonates. Regulatory T cells and lymphocyte subsets were determined using a flow cytometer. Student's t-test for independent samples was used to compare differences between AGA and SGA neonates.
Regulatory T cells in cord blood were increased in the SGA group compared with normal controls (p=0.041). However, cytotoxic T cells in cord blood were significantly decreased in the SGA group compared with normal controls (p=0.007).
This is the first study to compare the distribution of lymphocyte subsets including regulatory T cells in cord blood between AGA neonates and SGA neonates.
Yonsei medical journal 05/2015; 56(3):798-804. DOI:10.3349/ymj.2015.56.3.798 · 1.29 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Objective
This study aimed to evaluate the placental weight, volume, and density, and investigate the significance of placental ratios in pregnancies complicated by small for gestational age (SGA), preeclampsia (PE), and gestational diabetes mellitus (GDM).
Two hundred and fifty-four pregnant women were enrolled from August 2005 through July 2013. Participants were divided into four groups: control (n=82), SGA (n=37), PE (n=102), and GDM (n=33). The PE group was classified as PE without intrauterine growth restriction (n=65) and PE with intrauterine growth restriction (n=37). Birth weight, placental weight, placental volume, placental density, and placental ratios including birth weight/placental weight ratio (BPW) and birth weight/placental volume ratio (BPV) were compared between groups.
Birth weight, placental weight, and placental volume were lower in the SGA group than in the control group. However, the BPW and BPV did not differ between the two groups. Birth weight, placental weight, placental volume, BPW, and BPV were all significantly lower in the PE group than in the control group. Compared with the control group, birth weight, BPW, and BPV were higher in the GDM group, whereas placental weight and volume did not differ in the two groups. Placental density was not significantly different among the four groups.
Placental ratios based on placental weight, placental volume, placental density, and birth weight are helpful in understanding the pathophysiology of complicated pregnancies. Moreover, they can be used as predictors of pregnancy complications.
[Show abstract][Hide abstract] ABSTRACT: The Sysmex XN (XN) modular system (Sysmex, Kobe, Japan) is a new automated hematology analyzer equipped with different principles from its previous version, Sysmex XE-2100. We compared the performances of Sysmex XN and XE-2100 in umbilical cord blood (CB) specimens.
In 160 CB specimens, complete blood count (CBC) parameters and white blood cells (WBC) differentials were compared between the two analyzers. Their flagging performances for blasts, abnormal/atypical lymphocytes, immature granulocytes and/or left-shift (IG), and nucleated red blood cells (NRBC) counts were compared with manual counts. For the blast flagging, Q values by Sysmex XN were further compared with manual slide review.
Sysmex XN and XE-2100 showed high or very high correlations for most CBC parameters but variable correlations for WBC differentials. Compared with XE-2100, XN showed significantly different flagging performances for blasts, abnormal/atypical lymphocytes, and IG. The flagging efficiency for blasts was significantly better on Sysmex XN than on XE-2100 (85.0% vs. 38.8%): Sysmex XN showed a remarkably increased specificity of blast flag, compromising its sensitivity of blast flag. Among the 24 specimens with blasts (range, 0.5%–1.5%), only one (4.2%) showed a positive Q value.
This study highlighted the remarkable differences of flagging performances between Sysmex XN and XE-2100 in CB specimens. The Sysmex XN modular system seems to be a suitable and practical option for the CB specimens used for hematopoietic stem cell transplantation as well as for the specimens from neonates.
Clinical Chemistry and Laboratory Medicine 06/2014; 52(12). DOI:10.1515/cclm-2014-0392 · 2.71 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Objective
To evaluate the platelet distribution width (PDW) and other platelet indices as potential severity markers of preeclampsia (PE).
Study design: A total of 935 pregnant women who had received continuous prenatal care and had undergone delivery were included. The participants were classified into 3 groups: healthy pregnant women (n = 816), pregnant women with mild PE (n = 59), and pregnant women with severe PE (n = 60). Blood samples were collected during antenatal care or at the time of admission, and the platelet indices were compared among the three groups.
Among the three groups, the platelet count and plateletcrit decreased as the disease progressed. The mean platelet volume and the PDW, however, increased as the disease progressed. When compared to the levels of other platelet indices, the PDW showed significant elevation in the severe PE group. In the mild and severe PE groups, the PDW was statistically correlated with the mean arterial pressure (MAP) (r = 0.231, p < 0.05), whereas other platelet indices were not. In the receiver operating characteristics curve analysis, the area under the curve of the PDW to predict severe PE was 0.74.
Among platelet indices, the PDW is significantly higher in women with severe PE than in women with mild PE, and is positively correlated with the MAP. Therefore, the PDW can serve as a candidate marker for predicting the severity of PE.
European journal of obstetrics, gynecology, and reproductive biology 04/2014; 175(1). DOI:10.1016/j.ejogrb.2013.12.036 · 1.70 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Uterine arteriovenous malformation (AVM) is rare but potentially life-threatening from excessive vaginal bleeding. All uterine AVMs reported to date have been found in the endometrial or myometrial layers. Here we present a patient with a subserosal type AVM on the fundus of uterus, which spontaneously ruptured.
[Show abstract][Hide abstract] ABSTRACT: Objectives:
The aim of this study was to evaluate the value of imaging analysis of cervical elastography to predict successful induction of labor in nulliparous women at term.
Successful labor induction was defined as onset of active labor within 9 hours or delivery within 24 hours. The Bishop score, cervical length, and cervical elastographic parameters, including cervical area, mean elastographic index, and cervical hard area, were measured and analyzed by the image analyzer.
The areas under the curves for the cervical length, cervical area, Bishop score, mean elastographic index, and cervical hard area were 0.63, 0.64, 0.47, 0.68, and 0.70, respectively, for onset of active labor within 9 hours and 0.70, 0.68, 0.63, 0.71, and 0.76 for delivery within 24 hours. The combination of cervical length and elastographic data was more predictable for successful labor induction (P < .05).
Imaging analysis of cervical elastography is available to predict successful induction of labor.
Journal of ultrasound in medicine: official journal of the American Institute of Ultrasound in Medicine 06/2013; 32(6):937-946. DOI:10.7863/ultra.32.6.937 · 1.54 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of our study was to establish whether there is a correlation between the size of placental lakes and adverse pregnancy outcome.
Target ultrasonography for diagnosis of placental lake was performed in the 2nd trimester of pregnancy and followed up in the 3rd trimester. Placental lakes were defined as homogenous sonolucent avillous lesions greater than 2 cm × 2 cm in diameter. The 109 pregnant women enrolled in this study were divided into four groups according to the size and change in size of placental lakes. Clinical characteristics and pregnancy outcomes in each group were compared.
Some placental lakes decreased and disappeared, whereas others persisted. There were no significant differences in clinical characteristics among the four investigated groups. Fetal small-for-gestational-age status was significantly correlated with large placental lakes, compared to small.
Large placental lakes were correlated with the fetal status of small for gestational age. Therefore, if a large placental lake is identified in the 2nd trimester of pregnancy, appropriate surveillance should be considered for the remainder of the pregnancy.
European journal of obstetrics, gynecology, and reproductive biology 05/2012; 162(2):139-43. DOI:10.1016/j.ejogrb.2012.02.015 · 1.70 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Objective: This study aimed to establish reference intervals for lymphocyte subsets including CD4(+)CD25(high)FoxP3(+) regulatory T-cells (Tregs) in umbilical cord blood. Methods: Umbilical cord blood was obtained after birth from 120 healthy full-term neonates, who were born between November 2010 and November 2011. Lymphocyte subsets including Tregs were analysed using flow cytometer (Beckman Coulter, Fullerton, CA, USA), and the reference intervals were defined using non-parametrical percentile methods according to the Clinical and Laboratory Standard Institute guideline (C28-A3). Results: The reference intervals for lymphocyte subsets were: helper T-cells (CD3(+)/CD4(+)), 15.40-70.06%; cytotoxic T-cells (CD3(+)/CD8(+)), 9.65-34.28%; B-cells (CD19(+)), 4.50-29.59%; and natural killer cells (CD3(-)/CD16(+)/CD56(+)), 1.42-28.03%. The reference interval for Tregs was 0.35-9.07%. Conclusions: This study provides the reference intervals for lymphocyte subsets including Tregs in umbilical cord blood from healthy full-term neonates. These results could be used as fundamental data for clinical laboratory tests as well as future researches.
The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 02/2012; 25(10):2058-61. DOI:10.3109/14767058.2012.666591 · 1.37 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of this study was to investigate the potential prognostic factors and it's relating factors of patients who were hospitalized for management of preterm labor.
[Show abstract][Hide abstract] ABSTRACT: To investigate the expression pattern of CXC chemokine ligand-12 (CXCL12) in the placentae of normal and pre-eclamptic women.
Twenty-five women with severe pre-eclampsia and 30 normotensive women, matched for gestational age, were enrolled in the study. Placental tissue from each woman was collected following delivery by caesarean section. Quantitative reverse transcription polymerase chain reaction, Western blot analysis and immunohistochemical staining were performed for mRNA expression, quantification and tissue localization of CXCL12 in each placenta.
CXCL12 expression was greater in pre-eclamptic placentae compared with normal placentae. CXCL12 was detected in most placental tissue cells by immunohistochemical staining. CXCL12 immunoreactivity was significantly greater in syncytiotrophoblasts of pre-eclamptic placentae compared with normal placentae. However, there was no significant difference in CXCL12 immunoreactivity in other tissues between the two groups.
CXCL12 expression is significantly greater in the placentae of pre-eclamptic women compared with normal women. This may represent part of a compensatory mechanism for pre-eclampsia.
European journal of obstetrics, gynecology, and reproductive biology 11/2011; 160(2):137-41. DOI:10.1016/j.ejogrb.2011.10.007 · 1.70 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study was conducted to establish the relationship between three-dimensional (3-D) cervical volume and the risk of spontaneous preterm birth. A total of 391 asymptomatic women with singleton pregnancies were prospectively evaluated two-dimensional cervical length and 3-D cervical volume at 20-24 weeks of gestation. We assessed the relationship between cervical factors and the risk for preterm birth before 36 weeks of gestation. Logistic regression analysis demonstrated that not only cervical length (odds ratio [OR], 0.94; 95% confidence interval [CI], 0.88-0.99, p = 0.002), but also cervical volume (OR, 0.26; 95% CI, 0.11-0.61, p = 0.045) was independent predictor of preterm birth. A combined approach in which a gravida was regarded as positive with a short cervical length (≤28 mm) or a small cervical volume (≤20 cm(3)) had a sensitivity of 57.1% for preterm birth. Screening combining cervical length and volume may provide a better prediction of preterm birth.
Ultrasound in medicine & biology 07/2011; 37(7):1039-45. DOI:10.1016/j.ultrasmedbio.2011.04.010 · 2.21 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of our study was to determine the difference of cellular prion protein (PrP(C)) expression in the placentas of women with normal and preeclamptic pregnancies.
Retrospective study using banked placental tissue samples.
Twenty women with severe preeclampsia (preeclampsia group) and 20 gestational age-matched normotensive women (normal group).
Placental tissue from each woman collected at the time of cesarean section.
Quantitative reverse transcription polymerase chain reaction (RT-PCR), western blot analysis, and immunohistochemical and immunofluorescent staining for mRNA expression, quantification and tissue localization of PrP(C) in each placenta.
Increased expression of PrP(C) in preeclamptic placenta.
Compared with the normal group, PrP(C) and its mRNA were highly expressed in preeclampsia (each, p < 0.001). In immunohistochemical and immunofluorescent staining, PrP(C) was present at the syncytiotrophoblast, cytotrophoblast, endothelial cell, stroma, and decidua of all placentas. When the PrP(C) immunoreactivity in each tissue was compared, PrP(C) in endothelial cell, stroma, and deciduas was weakly expressed, and there was no difference of its expression between two groups. But, the intensity of PrP(C) expression in syncytiotrophoblast and cytotrophoblast was much higher in preeclampsia than normal.
The increased expression of PrP(C) in preeclamptic placenta may be a compensatory phenomenon for preeclampsia related conditions. Furthermore, this change in preeclamptic placenta may give an explanation for placental response to overcome the preeclamptic conditions.
[Show abstract][Hide abstract] ABSTRACT: Adenomyosis has been well known to be associated with infertility, spontaneous rupture of the uterus during labor in a primiparous woman, spontaneous preterm labor, preterm premature rupture of membranes (PPROM), and delayed postpartum hemorrhage. We recently experienced a case of preterm delivery at 29 gestational weeks in a primigravid woman with uterine adenomyosis. We report the case of preterm delivery accompanied by various complications such as uncontrolled pain, preterm labor, and oligohydramnios in a woman with uterine adenomyosis.
[Show abstract][Hide abstract] ABSTRACT: A 31-year-old premenopausal woman, who was not sexually active, underwent uterine fibroid embolization for a suspected symptomatic uterine fibroid. Twelve months later, the patient underwent surgery due to regrowth of the mass on follow-up ultrasound. The pathological report showed a low-grade endometrial stromal sarcoma.
European Journal of Radiology Extra 04/2009; 70(1). DOI:10.1016/j.ejrex.2008.10.011
[Show abstract][Hide abstract] ABSTRACT: Endometrial stromal sarcoma (ESS) that arises from extrauterine endometriosis is a rare form of malignancy. We report the case of a 37-year-old ESS patient with extrauterine endometriosis who was treated with ifosfamide/cisplatin chemotherapy. A woman presented with epigastric pain and abdominal distension. Computed tomography imaging revealed a profuse amount of ascites, including a 12.4×12.3 cm sized posterior cul-de-sac mass composed of solid and cystic components. Cytoreductive surgery was performed to remove the mass and the histopathologic findings indicated ESS associated with extrauterine endometriosis. Six cycles of combination chemotherapy [ifosfamide (5 g/m2) with mesna (1 g/m2) and cisplatin (50 mg/m2) (IP)] were administered. After a six-month of disease-free interval, recurrent ESS developed in the pelvic cavity and in both lung fields. Megace medication decreased tumor marker CA-125 for six weeks. However, the patient expired sixteen months after the cytoreductive surgery. ESS associated with extrauterine endometriosis showed response to IP chemotherapy and megace.
Korean Journal of Gynecologic Oncology 01/2008; 19(1). DOI:10.3802/kjgo.2008.19.1.87