Edna Sayuri Suyenaga

Publications (2)3.03 Total impact

• Article: Pharmacological evaluation of Copaifera multijuga oil in rats.

  Cristine Kobayashi, Tiago Oselame Fontanive, Barbara Grade Enzweiler, Laura Renata de Bona, Thalita Massoni, Miriam Anders Apel, Amélia Teresinha Henriques, Marc François Richter, Patrícia Ardenghi, Edna Sayuri Suyenaga
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  ABSTRACT: Copaiba oil is an oleoresin made up of resin acids and volatile compounds, and it is obtained by tapping the trunks of trees that are members of the Copaifera L. (Leguminoseae) genus and are found in tropical parts of Latin America. This study analyzed the chemical composition of Copaifera multijuga Hayne oil and conducted preclinical trials to investigate anti-inflammatory effects and any action it may have on the central nervous system (CNS) of rats. The chemical analysis was carried out using gas chromatography with mass spectroscopy. Anti-inflammatory activity was measured by leucocytes mobilization, by chemotaxis assay in Boyden's chamber, and by pleurisy model in rats. CNS effect was determined by plus maze and open-field assays. The statistical test applied was analysis of variance (ANOVA) followed by Tukey's test or ANOVA followed by Duncan's test. The oil was composed of sesquiterpenes with the predominance of β-caryophyllene (36.0%), followed by α-copaene (18.8%), β-bisabolene (8.5%), and α-trans-bergamotene (7.0%). Data demonstrated that at 100 and 200 mg/kg doses and at a concentration of 200 μl/ml copaiba essential oil presented anti-inflammatory effects both in vivo and in vitro based on reduced leukocyte migration to the rats' pleural cavity and to the chemotactic agent lipopolysaccharide solution, respectively. During the experiments investigating CNS effects, locomotive and exploratory activities were reduced and the animals' anxiety increased at 100 and 200 mg/kg. The results obtained suggest that copaiba oil has an interesting anti-inflammatory effect and important effect on the CNS.
  Pharmaceutical Biology 03/2011; 49(3):306-13. · 0.88 Impact Factor
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  Article: Appraisal of the antichemotactic activity of flavonoids on polymorphonuclear neutrophils.

  Edna Sayuri Suyenaga, Eduardo Luis Konrath, Roger Remy Dresch, Miriam Anders Apel, José Angelo Zuanazzi, Célia Gervásio Chaves, Amélia Teresinha Henriques
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  ABSTRACT: Flavonoids are polyphenols that are ubiquitous in plants and frequently consumed in the diet. They are suggested to have many beneficial actions on human health, including anti-inflammatory activity. Their properties have been studied in a number of cell types, but little is known about their effects on neutrophil biology. Consequently, we selected 25 flavonoids with different structural features to evaluate their in vitro inhibition of rat polymorphonuclear neutrophil (PMN) chemotaxis, employing a modified Boyden chamber. Migratory activity was measured towards a chemotactic stimulant, formyl-Met-Leu-Phe or lipopolysaccharide. Furthermore, the cytotoxic effect of flavonoids on PMNs was determined by the release of cytosolic lactate dehydrogenase (LDH). Ten flavonoids significantly retarded the migration of PMNs with at least one of the concentrations tested in a range between 0.625 and 100 µM; the best antichemotactic agents were flavone, flavonol, quercetin and rutin. None of the flavanones evaluated presented any significant inhibition of migration in this assay. Our findings indicated that non-hydroxylated flavones possess a better antichemotactic activity when compared to flavones with hydroxy groups. The presence of a sugar moiety in rutin did not produce any increase in this effect, when compared to the respective aglycone analogue. Finally, none of the flavonoids exhibited cell toxicity and for many of these flavonoids this is the first report of the inhibition of PMN chemotaxis.
  Planta Medica 11/2010; 77(7):698-704. · 2.15 Impact Factor