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ABSTRACT: Changes in water potential, growth elongation, photosynthesis of three-leaf-old seedlings of maize inbred line YQ7-96 under water deficit (WD) for 0.5, 1 and 2 h and re-watering (RW) for 24 h were characterized. Gene expression was analyzed using cDNA microarray covering 11,855 maize unigenes. As for whole maize plant, the expression of WD-regulated genes was characterized by up-regulation. The expression of WD-regulated genes was categorized into eight different patterns, respectively, in leaves and roots. Newly found and WD-affected cellular processes were metabolic process, amino acid and derivative metabolic process and cell death. A great number of the analyzed genes were found to be regulated specifically by RW and commonly by both WD and RW, respectively, in leaves. It is therefore concluded that (1) whole maize plant tolerance to WD, as well as growth recovery from WD, depends at least in part on transcriptional coordination between leaves and roots; (2) WD exerts effects on the maize, especially on basal metabolism; (3) WD could probably affect CO(2) uptake and partitioning, and transport of fixed carbons; (4) WD could likely influence nuclear activity and genome stability; and (5) maize growth recovery from WD is likely involved in some specific signaling pathways related to RW-specific responsive genes.
Theoretical and Applied Genetics 07/2011; 123(6):943-58. · 3.30 Impact Factor
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ABSTRACT: Cis-cinnamic acid (CA) is one of many cis-phenylpropanoids found in both monocots and dicots. It is produced in planta via sunlight-mediated isomerization of trans-cinnamic acid. This pair of isomers plays a differential role in regulation of plant growth. A functional proteomics approach has been adopted to identify genes of cis/trans-CA mixture-enhanced expression. Out of 1,241 proteins identified by mass spectrometry, 32 were CA-enhanced and 13 repressed. Further analysis with the molecular biology approach revealed 2 cis-CA (Z usammen-CA)-E nhanced genes, named ZCE1 and ZCE2, which encode members of the major latex protein-like (MLPL) gene family. The transcript accumulation of both genes is positively correlated with the amount of cis-CA applied externally, ranging from 1 to 100 μM. ZCE1 transcript accumulation is enhanced largely by cis-CA and slightly by other cis-phenylpropanoids. Treatment of several well-characterized plant growth regulator perception-deficient mutants with cis-CA is able to promote ZCE1 transcript accumulation, suggestive of distinct signaling pathways regulating cis-CA response. The zce1 loss-of-function mutant produced via the RNA-interference technique produces an earlier bolting phenotype in Arabidopsis, suggesting that ZCE1 plays a role in promoting vegetative growth and delay flowering.
Plant Molecular Biology 02/2011; 75(4-5):481-95. · 4.15 Impact Factor
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ABSTRACT: So far, the tolerance mechanisms of plant tolerance to aluminium (Al) toxicity are still controversial. This will require an interdisciplinary approaches integrating genetic, molecular, and physiological investigations. To lay a foundation for studying mechanism of whole maize plant tolerance to Al stress (AS) and growth recovery by removal of Al toxicity (RAT), temporal physiological responses and comparative transcriptional profiling of roots and leaves of maize inbred line YQ7-96 under AS with 0.5 mmol L−1 AlCl3·6H2O and RAT were studied. In addition to toxicity towards maize, AS can lead to water deficit effect on whole maize plant. There exists independence of transcriptional response mechanisms between leaves and roots when whole maize plant responds to AS and then undergoes RAT treatment. Amino acid metabolism pathways play a very important role in Al detoxification. There exist some signaling pathways parallel to human's ones, including Wnt, ErbB, TGF-beta and Jak-STAT and the type III secretion system, which probably govern maize response to AS and RAT. The data presented in this study are very helpful to understanding of the Al-tolerant mechanisms of whole maize plant.
Environmental and Experimental Botany 69(2):158-166. · 2.98 Impact Factor
