Dana C Price

Publications of Dana C Price

• Single cell genome analysis supports a link between phagotrophy and primary plastid endosymbiosis.

  Authors: Debashish Bhattacharya, Dana C Price, Hwan Su Yoon, Eun Chan Yang, Nicole J Poulton, Robert A Andersen, Sushma Parankush Das

  Scientific reports. 01/2012; 2:356.

  Two cases of primary plastid endosymbiosis are known. The first occurred ca. 1.6 billion years ago and putatively gave rise to the canonical plastid in algae and plants. The second is restricted to aTwo cases of primary plastid endosymbiosis are known. The first occurred ca. 1.6 billion years ago and putatively gave rise to the canonical plastid in algae and plants. The second is restricted to a genus of rhizarian amoebae that includes Paulinella chromatophora. Photosynthetic Paulinella species gained their plastid from an α-cyanobacterial source and are sister to plastid-lacking phagotrophs such as Paulinella ovalis that ingest cyanobacteria. To study the role of feeding behavior in plastid origin, we analyzed single-cell genome assemblies from six P. ovalis-like cells isolated from Chesapeake Bay, USA. Dozens of contigs in these cell assemblies were derived from prey DNA of α-cyanobacterial origin and associated cyanophages. We found two examples of horizontal gene transfer (HGT) in P. ovalis-like nuclear DNA from cyanobacterial sources. This work suggests the first evidence of a link between feeding behavior in wild-caught cells, HGT, and plastid primary endosymbiosis in the monophyletic Paulinella lineage.

• Single-cell genomics reveals organismal interactions in uncultivated marine protists.

  Authors: Hwan Su Yoon, Dana C Price, Ramunas Stepanauskas, Veeran D Rajah, Michael E Sieracki, William H Wilson, Eun Chan Yang, Siobain Duffy, Debashish Bhattacharya

  Science (New York, N.Y.). 05/2011; 332(6030):714-7.

  Whole-genome shotgun sequence data from three individual cells isolated from seawater, followed by analysis of ribosomal DNA, indicated that the cells represented three divergent clades ofWhole-genome shotgun sequence data from three individual cells isolated from seawater, followed by analysis of ribosomal DNA, indicated that the cells represented three divergent clades of picobiliphytes. In contrast with the recent description of this phylum, we found no evidence of plastid DNA nor of nuclear-encoded plastid-targeted proteins, which suggests that these picobiliphytes are heterotrophs. Genome data from one cell were dominated by sequences from a widespread single-stranded DNA virus. This virus was absent from the other two cells, both of which contained non-eukaryote DNA derived from marine Bacteroidetes and large DNA viruses. By using shotgun sequencing of uncultured marine picobiliphytes, we revealed the distinct interactions of individual cells.

• Sea slug kleptoplasty and plastid maintenance in a metazoan.

  Authors: Karen N Pelletreau, Debashish Bhattacharya, Dana C Price, Jared M Worful, Ahmed Moustafa, Mary E Rumpho

  Plant physiology. 02/2011; 155(4):1561-5.