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ABSTRACT: The choice of the short-term extender is important to preserve the quality of the diluted South African indigenous Kolbroek boar semen during short-term storage prior to artificial insemination. The aim of this study was to identify the suitable extender for short-term storage of Kolbroek boar semen at 17°C. Five ejaculates were collected separately from five Kolbroek boars using the gloved-hand technique in a 300-mL glass beaker. The filtered sperm fraction were sealed with a gauze filter inside a prewarmed (39°C) insulated thermos flask. Following semen collection, the semen was evaluated for macroscopic characteristics; semen volume, pH, and concentration. Sperm cells morphology was evaluated using eosin-nigrosin staining solution using fluorescent microscope system. Only ejaculates with >75% motile sperm and <15 abnormalities were used. The semen was pooled and diluted with five different short-term extenders, namely: Beltsville Thawing Solution (BTS), Kobidil(+), Hungarian Standard, Citrate, and Tris-based extenders at a ratio of 1:1 (v/v). Sperm cells motility parameters were evaluated using a sperm class analyzer (SCA) at 0, 3, 24, and 48h intervals. The data were analysed by SPSS. The results demonstrated that sperm cell motility rate did not differ when stored at 0 (88.9±8.3) and 3 (93.8±4.4)h irrespective of extender. However, semen that was diluted with Hungarian extender and Tris-based extender maintained higher proportion of sperm cells motility (90.0±2.5 and 87.8±4.8, respectively) for a longer period during storage for 24h as compared with the other extenders. In conclusion, the study demonstrated that prolonged storage of boar sperm cells reduces motility rates. However; Hungarian Standard and Tris-based extenders preserved sperm motility rate better than the other extenders when stored up to 48h at 17°C. Thus, this study suggests that Kolbroek sperm cells can be transported within 24 to 48h in South African regions without adversely affecting motility rates when diluted with Hungarian Standard and Tris-based extender at 17°C without cryopreserving them.
Reproduction Fertility and Development 01/2011; 23(1):144. · 2.58 Impact Factor