Publications (2)10.88 Total impact
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Article: In vivo visualization of gold-loaded cells in mice using x-ray computed tomography.
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ABSTRACT: The ability to perform cell tracking using x-ray computed tomography combined with gold nanoparticles has been demonstrated recently on ex vivo samples using different malignant and nonmalignant cell lines. Here we proved the concept of the method for in vivo assessment in a small-animal model of malignant brain tumors. The limitations of the method due to radiation dose constraints were investigated using Monte Carlo simulations. Taking into consideration different x-ray entrance doses and the spatial resolution, the visibility of the cell clusters was evaluated. The results of the experiments conducted on mice implanted with F98 tumor cells confirmed the prediction of the Monte Carlo calculations. Small clusters of cells exogenously loaded with gold nanoparticles could be visualized using our in vivo method.Nanomedicine: nanotechnology, biology, and medicine 07/2012; · 5.44 Impact Factor -
Article: Gold nanoparticle labeling of cells is a sensitive method to investigate cell distribution and migration in animal models of human disease.
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ABSTRACT: The ability to track cells in small-animal models of human disease is important because it gives the potential to improve our understanding of the processes of disease progression as well as our understanding of the therapeutic effects of interventions. In this study gold nanoparticles have been used as a permanent marker of implanted normal and malignant cell grafts in combination with a suitable x-ray apparatus. Using x-ray computed tomography the micrometric three-dimensional distribution of these marked cells could be displayed with penetration depth, high cell sensitivity and high spatial resolution in rodent models of human diseases. In principle the method allows quantification of cell numbers at any anatomical location over time in small animals.Nanomedicine: nanotechnology, biology, and medicine 02/2011; 7(5):647-54. · 5.44 Impact Factor
