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ABSTRACT: The scope of this research lies in the effects of indocyanine green (ICG) on the near-infrared optical properties and optical coherence tomographic image of cerebral blood vessel in vivo in rats. The skulls of SD rats were opened under nembutal anesthesia to expose and mark the middle cerebral artery. The reflectance spectra of middle cerebral artery were monitored by Vis/ NIR spectrometer and the optical attenuation coefficients of middle cerebral artery were detected by optical coherence tomography (OCT) when indocyanine green was administrated intravenously through tail veins. It was shown that the reflectance spectra of middle cerebral artery could provide guidance for OCT image, where characteristic changes appeared around 800 nm, an absorption peak of indocyanine green. Additionally, significant difference (p<0.01) was observed between the optical attenuation coefficients of middle cerebral artery with and without indocyanine green, which were 24.692 +/- 1.471 and 15.088 +/- 1.602, respectively. It was concluded that indocyanine green, as an optical contrast agent to enhance detection of cerebral artery by the reflectance spectra and OCT imaging, has the potential for monitoring and imaging of cerebral blood vessels.
Guang pu xue yu guang pu fen xi = Guang pu 07/2012; 32(7):1766-70. · 0.84 Impact Factor
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ABSTRACT: In this study, to analyze the influence of the brightness value of the supranasal point and the apex nasi on their dominant wavelength and excitation purity according to the spectrocolorimetry data of the supranasal point and the apex nasi in healthy adults that were collected based on optical spectrum colorimetry.
A total of 516 healthy adults were taken as the research subjects. The brightness, dominant wavelength and excitation purity values of the supranasal point and the apex nasi during the complexion inspection of subjects were calculated. This was based on the visible reflection spectrum, and the linear correlation/regression analysis between the brightness Y value and the dominant wavelength or excitation purity value.
There was no correlation between the brightness Y value and the dominant wavelength of the normal supranasal point and the apex nasi; however, there was negative correlation between the brightness Y value and the excitation purity of the normal supranasal point and apex nasi.
During the complexion inspection, the brightness Y value would not influence the dominant wavelength value, indicating that whiteness and/or blackness would not influence the normal individual complexion. However, the brightness Y value would influence the excitation purity of the supranasal point and the apex nasi, and the degree of saturation should be referred to as the brightness. This research provides a basic reference for diagnosing facial complexion in traditional Chinese medicine.
Journal of Chinese Integrative Medicine 12/2011; 9(12):1333-8.
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ABSTRACT: Using the data from normal tongue and lip colors of normal people which were collected by the visible reflection spectrum, we analyzed the colorimetric parameters of tongue and lip colors.
In this study, 516 healthy students aging from 19 to 26 from the colleges and universities of Guangdong Province of China were taken as research subjects. After collecting the data of tongue and lip colors of the 516 subjects using visible reflectance spectroscopy, CIE XYZ tristimulus values as defined by the International Commission on Illumination in 1964 were calculated, and the colorimetric parameters of the normal tongue and lip colors were obtained, such as the CIE 1964 chromaticity coordinate, brightness, dominant wavelength and excitation purity.
The results of CIE 1964 chromaticity diagram calculated on the visible reflection spectrum showed that the normal tongue color chromaticity coordinate x(10) was 0.341 3±0.008 5 and y(10) was 0.332 6±0.005 1, and the normal lip color chromaticity coordinate x(10) was 0.357 7±0.009 2 and y(10) was 0.338 3±0.005 7; the brightness Y values of the normal tongue color and lip colors were 17.96±3.78 and 19.78±3.72, the dominant wavelength values of the normal tongue color and lip color were (626.3±51.6) nm and (600.4±18.2) nm, and the excitation purity values of the normal tongue color and lip color were 0.083±0.031 and 0.144±0.036, respectively.
Application of the visible reflection spectrum is a standard way to collect colorimetric data for inspection of the complexion. The investigation of chromaticity coordinates, brightness, dominant wavelength and excitation purity of the normal tongue and lip colors may offer the basic reference for diagnosing morbid complexion on the tongue and lip colors in traditional Chinese medicine.
Journal of Chinese Integrative Medicine 09/2011; 9(9):948-54.
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ABSTRACT: Substance P (SP) is a neuropeptide that plays an important role in inflammation, respiration, pain, aggression, anxiety, and learning and memory mainly through its high affinity neurokinin 1 receptor (NK1R). The marginal division (MrD) is a pan-shaped subdivision in the caudomedial margin of the neostriatum in the mammalian brain and is known to be involved in learning and memory. We studied the expression of SP, NK1R and NK1R mRNA in the rat striatum by immunohistochemistry, immunofluorescence and in situ hybridization, and found that the levels of SP, NK1R protein and NK1R mRNA were high in the cell bodies, fibers and terminals of neurons in the neostriatum, especially in the MrD. Knocking down NK1R activity in the MrD by using an antisense oligonucleotide against NK1R mRNA inhibited learning and memory in a Y-maze behavioral test. Our results show that NK1R mediates the role of SP in the MrD in learning and memory.
Neurochemical Research 05/2011; 36(10):1896-902. · 2.24 Impact Factor
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ABSTRACT: In this study, optical data on color of tongue tips were collected by using a visible reflection spectrum system. Quantitative method of tongue inspection and classification of tongue states including pale, pale red, red and crimson manifestations were investigated.
Chromaticity coordinates of the tongue tips from 98 subjects were calculated on the basis of the visible reflection spectrum. The tongue color was quantified and classified by the visible reflection spectrum in the range from 590 nm to 780 nm, based on the higher red-band energy distribution on the tongue tip color.
It was found that comparison and analysis of tongue tip color from different samples would be well-suited after spectrum vector normalization, and the difference of tongue tip color between different samples could be observed easily when the tongue tip optical data were sited in CIE 1931 chromaticity diagram. The tongue tip colors were analyzed quantitatively and classified by the spectral energy intensity at the wavelength of 670 nm and (or) spectral energy ratio of red bank from 590 nm to 780 nm. The results of classification showed that there was sound corresponding accuracy with the clinical diagnosis of pale tongue, pale red tongue, red tongue and crimson tongue.
In this study, based on the collection of the information data on tongue tip characteristics with the visible spectrum from 380 nm to 780 nm, the quantitative analysis of tongue inspection for pale tongue, pale red tongue, red tongue and crimson tongue was established. The quantitative value for tongue tip color is an effective method for classification of the condition of the tongue tip, which provides a useful tool for the modernization of tongue inspection in traditional Chinese medicine.
Journal of Chinese Integrative Medicine 01/2011; 9(1):29-35.
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ABSTRACT: Acupoint was reaction of viscera and its optical parameter was an important characteristic of tissue. In the present work, in order to discuss specificity of acupuncture on the response of qi and blood in human body, we compared the diffuse reflectance of Taichong (LV3), Taibai (SP3), and Chongyang (ST42) before, during and after menstruation. All the acupoints had the same shape of the spectrum and troughs were all at 423.16, 544.06 and 577.47 nm. The values of reflectance during menstruation were greater than before and after menstruation, especially for SP3 and ST42 (P < 0.05). SP3 and ST42 were more sensitive to the changes of qi and blood than LV3, and it was asymmetric and we found no evidence for the imbalance of the left side and right. These results indicated that acupoint diffuse reflectance changed with qi and blood, and that SP3 and ST42 had close relationship with menstruation. Diffuse reflectance may be possibly used in the quantitative analysis of qi and blood.
Guang pu xue yu guang pu fen xi = Guang pu 12/2010; 30(12):3338-42. · 0.84 Impact Factor
