C Bizet

Publications of C Bizet

• High Inter-Laboratory Reproducibility of MALDI-TOF Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria.

  Authors: A Mellmann, F Bimet, C Bizet, A. D. Borovskaya, R. R. Drake, U Eigner, A M Fahr, Y. He, E. N. Ilina, M Kostrzewa, T Maier, L Mancinelli, W. Moussaoui, G Prévost, L Putignani, C. L. Seachord, Y. W. Tang, D Harmsen

  Journal of clinical microbiology. 09/2009;

  MALDI-TOF MS has emerged as rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (eightMALDI-TOF MS has emerged as rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (eight laboratories) achieved 98.75% inter-laboratory reproducibility. Only six of 480 samples were misidentified due to interchanges (4 samples) or contamination (1), or not identified because of insufficient signal intensity (1).

• Microbacterium binotii sp. nov., isolated from human blood.

  Authors: D Clermont, S Diard, C Bouchier, C Vivier, F Bimet, L Motreff, M Welker, W Kallow, C Bizet

  International journal of systematic and evolutionary microbiology. 06/2009; 59(Pt 5):1016-22.

  Two strains of non-spore-forming, rod-shaped, Gram-positive bacteria, CIP 101303(T) and CIP 102116, were isolated from human blood in 1976 and 1977, respectively. These strains had chemotaxonomicTwo strains of non-spore-forming, rod-shaped, Gram-positive bacteria, CIP 101303(T) and CIP 102116, were isolated from human blood in 1976 and 1977, respectively. These strains had chemotaxonomic markers that were consistent with classification in the genus Microbacterium, i.e. MK-10, MK-11 and MK-12 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, galactose, mannose and rhamnose as the cell-wall sugars and ornithine as the diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 70-72 mol%. Comparative 16S rRNA gene sequence studies revealed that strains CIP 101303(T) and CIP 102116 belonged to the genus Microbacterium and that they were related closely to Microbacterium halotolerans. The level of DNA-DNA relatedness showed that the two isolates represented a separate genomic species. Based on phenotypic and genotypic results, it is proposed that strains CIP 101303(T) and CIP 102116 be assigned to a novel species, Microbacterium binotii sp. nov. The type strain is CIP 101303(T) (=DSM 19164(T)).

• Bacillus aeolius DSM 15084T (=CIP 107628T) is a strain of Bacillus licheniformis.

  Authors: R Pukall, P Schumann, D Clermont, C Bizet

  International journal of systematic and evolutionary microbiology. 06/2008; 58(Pt 5):1268-70.

  Based on phenotypic properties, 16S rRNA gene sequence and MALDI-TOF analysis, strains DSM 15084T and CIP 107628T, deposited as the type strain of Bacillus aeolius, do not represent the original typeBased on phenotypic properties, 16S rRNA gene sequence and MALDI-TOF analysis, strains DSM 15084T and CIP 107628T, deposited as the type strain of Bacillus aeolius, do not represent the original type strain, strain 4-1T. It is therefore proposed that the Judicial Commission of the International Committee on Systematics of Prokaryotes places the name Bacillus aeolius on the list of rejected specific and subspecific epithets in names of species and subspecies of bacteria if a suitable replacement for the type strain or a neotype cannot be found within 2 years of publication of this Request.

• A sensitive and rapid method to determine the viability of freeze-dried bacterial cells.

  Authors: N Parthuisot, P Catala, P Lebaron, D Clermont, C Bizet

  Letters in applied microbiology. 02/2003; 36(6):412-7.

  AIMS: To determine the potential use of flow cytometry for viability asssessment of freeze-dried bacterial cells. METHODS AND RESULTS: Escherichia coli CIP 54.8T and Vibrio metschnikovii CIP 104262AIMS: To determine the potential use of flow cytometry for viability asssessment of freeze-dried bacterial cells. METHODS AND RESULTS: Escherichia coli CIP 54.8T and Vibrio metschnikovii CIP 104262 were analysed. The viability of freeze-dried cells resuspended in a nutrient broth was evaluated by culture whereas activity was determined by flow cytometry analysis of both esterase activity and cell death. Activity assessment by flow cytometry was found to be a rapid and good indicator of cell viability and was very efficient for quality control. For V. metschnikovii the fraction of active cells varies greatly depending on the freeze-drying procedure and within a given procedure. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Bacterial activity assessment by flow cytometry is very efficient for the control of freeze-dried cells.

• Identification of strains of Alcaligenes and Agrobacterium by a polyphasic approach.

  Authors: D Clermont, C Harmant, C Bizet

  Journal of clinical microbiology. 10/2001; 39(9):3104-9.

  The number of stable discriminant biochemical characters is limited in the genera Alcaligenes and Agrobacterium, whose species are consequently difficult to distinguish from one another byThe number of stable discriminant biochemical characters is limited in the genera Alcaligenes and Agrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. Moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, Alcaligenes xylosoxidans was transferred to the genus Achromobacter in 1998. Twenty-five strains of Achromobacter xylosoxidans, three strains of an Agrobacterium sp., five strains of an Alcaligenes sp., and four unnamed strains belonging to the Centers for Disease Control and Prevention group IVc-2 were examined. These strains were characterized by conventional tests, including biochemical tests. The assimilation of 99 carbohydrates, organic acids, and amino acids was studied by using Biotype-100 strips, and rRNA gene restriction patterns were obtained with the automated Riboprinter microbial characterization system after cleavage of total DNA with EcoRI or PstI restriction endonuclease. This polyphasic approach allowed the two subspecies of A. xylosoxidans to be clearly separated. Relationships between five strains and the Ralstonia paucula type strain were demonstrated. Likewise, three strains were found to be related to the Ochrobactrum anthropi type strain. We showed that substrate assimilation tests and automated ribotyping provide a simple, rapid, and reliable means of identifying A. xylosoxidans subspecies and that these two methods can be used as alternative methods to characterize unidentified strains rapidly when discriminant biochemical characters are missing.

• Molecular characterization of chromosomal class C beta-lactamase and its regulatory gene in Ochrobactrum anthropi.

  Authors: D Nadjar, R Labia, C Cerceau, C Bizet, A Philippon, G Arlet

  Antimicrobial agents and chemotherapy. 09/2001; 45(8):2324-30.

  Ochrobactrum anthropi, formerly known as CDC group Vd, is an oxidase-producing, gram-negative, obligately aerobic, non-lactose-fermenting bacillus of low virulence that occasionally causes humanOchrobactrum anthropi, formerly known as CDC group Vd, is an oxidase-producing, gram-negative, obligately aerobic, non-lactose-fermenting bacillus of low virulence that occasionally causes human infections. It is highly resistant to all beta-lactams except imipenem. A clinical isolate, SLO74, and six reference strains were tested. MICs of penicillins, aztreonam, and most cephalosporins tested, including cefotaxime and ceftazidime, were >128 microg/ml and of cefepime were 64 to >128 microg/ml. Clavulanic acid was ineffective and tazobactam had a weak effect in association with piperacillin. Two genes, ampR and ampC, were cloned by inserting restriction fragments of genomic DNA from the clinical strain O. anthropi SLO74 into pBK-CMV to give the recombinant plasmid pBK-OA1. The pattern of resistance to beta-lactams of this clone was similar to that of the parental strain, except for its resistance to cefepime (MIC, 0.5 ,micro/ml). The deduced amino acid sequence of the AmpC beta-lactamase (pI, 8.9) was only 41 to 52% identical to the sequence of other chromosomally encoded and plasmid-encoded class C beta-lactamases. The kinetic properties of this beta-lactamase were typical for this class of beta-lactamases. Upstream from the ampC gene, the ampR gene encodes a protein with a sequence that is 46 to 62% identical to those of other AmpR proteins and with an amino-terminal DNA-binding domain typical of transcriptional activators of the Lys-R family. The deduced amino acid sequences of the ampC genes of the six reference strains were 96 to 99% identical to the sequence of the clinical strain. The beta-lactamase characterized from strain SLO74 was named OCH-1 (gene, bla(OCH-I)).

• Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells.

  Authors: I Casadémont, C Bizet, D Chevrier, J L Guesdon

  Molecular and cellular probes. 09/2000; 14(4):233-40.

  Campylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack ofCampylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We cloned and sequenced a 1581-bp DNA fragment, IG02, isolated from a C. fetus genomic library. This fragment was used as a probe on DNAs extracted from C. fetus strains and other Campylobacter species: IG02 hybridized only with DNAs from C. fetus strains. A PCR-based test was developed for the detection of C. fetus. A pair of oligonucleotide primers was designed to amplify a 141-bp fragment of IG02. The amplified product was analysed by a non-radioactive sandwich hybridization in microtiter plate using a capture oligonucleotide and a biotin-labelled oligonucleotide for the detection. The combination of PCR and non-radioactive microplate hybridization is a convenient method for the rapid detection of C. fetus.

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• Lactobacillus species identification, H2O2 production, and antibiotic resistance and correlation with human clinical status.

  Authors: A Felten, C Barreau, C Bizet, P H Lagrange, A Philippon

  Journal of clinical microbiology. 04/1999; 37(3):729-33.

  Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation andLactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility to 19 antibiotics was tested by a diffusion method, and the MICs of benzylpenicillin, amoxicillin, imipenem, erythromycin, vancomycin, gentamicin, and levofloxacin were determined. A small number of species produced H2O2, and antibiotic susceptibilities were species related. Eighteen (90%) of the isolates were L. rhamnosus, one was L. paracasei subsp. paracasei, and one was L. crispatus. L. rhamnosus, L. paracasei subsp. paracasei isolates, and the type strains were neither H2O2 producers nor vancomycin susceptible (MICs, >/=256 microgram/ml). L. crispatus, as well as most of the type strains of lactobacilli which belong to the L. acidophilus group, was an H2O2 producer and vancomycin susceptible (MICs, <4 microgram/ml).

• Identification of Rhodococcus, Gordona and Dietzia species using carbon source utilization tests ("Biotype-100" strips).

  Authors: C Bizet, C Barreau, C Harmant, M Nowakowski, A Pietfroid

  Research in microbiology. 01/1998; 148(9):799-809.

  The "Biotype-100" identification system (BioMérieux, La Balme-Ies-Grottes, France) based on carbon source utilization was evaluated for its ability to discriminate among 10 species of Rhodococcus, 7The "Biotype-100" identification system (BioMérieux, La Balme-Ies-Grottes, France) based on carbon source utilization was evaluated for its ability to discriminate among 10 species of Rhodococcus, 7 species of Gordona and one species of Dietzia. The type strains of three species of Tsukamurella and 8 species of Nocardia were also included in the study. Results were compared with chemotaxonomic and conventional data. Carbon source utilization was shown to be reliable, rapid and easy to use when compared with standard identification methods. The 29 species tested were unambiguously separated by carbon source utilization tests. Rhodococcus equi was found to be heterogenous.

• Strains of Alcaligenes faecalis from clinical material.

  Authors: J Bizet, C Bizet

  The Journal of infection. 10/1997; 35(2):167-9.

  Six strains of Alcaligenes faecalis, unusually isolated from clinical material, are described. Alcaligenes faecalis is a Gram-negative catalase- and oxidase-positive, motile rod. It is commonly foundSix strains of Alcaligenes faecalis, unusually isolated from clinical material, are described. Alcaligenes faecalis is a Gram-negative catalase- and oxidase-positive, motile rod. It is commonly found in a watery environment and is rarely isolated from humans. The clinical and laboratory characteristics of the clinical A. faecalis isolates are presented.

• Characterization of the type strain of Campylobacter coli, CIP 70.80, by plasmid typing.

  Authors: V Stonnet, C Harmant, C Bizet, J L Guesdon

  International journal of systematic bacteriology. 08/1995; 45(3):592-4.

  A 1.9-kb plasmid DNA fragment from the type strain of Campylobacter coli, CIP 70.80, was used as a probe to characterize this type strain, other C. coli type strains obtained from several cultureA 1.9-kb plasmid DNA fragment from the type strain of Campylobacter coli, CIP 70.80, was used as a probe to characterize this type strain, other C. coli type strains obtained from several culture collections, and other C. coli strains. A specific hybridization pattern was obtained, and this pattern can be used to identify, characterize, and follow up C. coli type strains in culture collections.

• [Comparative susceptibility of Ochrobactrum anthropi, Agrobacterium tumefaciens, Alcaligenes faecalis, Alcaligenes denitrificans subsp. denitrificans, Alcaligenes denitrificans subsp. xylosidans and Bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams]

  Authors: C Bizet, J Bizet

  Pathologie-biologie. 05/1995; 43(4):258-63.

  Ochrobactrum anthropi, formerly known as "Achromobacter sp." or CDC group Vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). O. anthropi is a GramOchrobactrum anthropi, formerly known as "Achromobacter sp." or CDC group Vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). O. anthropi is a Gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. The susceptibility of 13 strains of O. anthropi was determined by agar diffusion method and compared to those of type strains of Agrobacterium tumefaciens, Alcaligenes faecalis, Alcaligenes denitrificans subsp. denitrificans, Alcaligenes denitrificans subsp. xylosoxydans and Bordetella bronchiseptica. The MICs of 20 antimicrobial agents confirmed the distinct phenotype susceptibility of O. anthropi. All the strains of O. anthropi are sensitive to imipenem, amikacin, gentamicin, netilmicin, nalidixic acid, pefloxacin, ciprofloxacin, tetracyclin, colistin, sulphonamides and rifampicin and resistant to ampicillin, amoxycillin + clavulanic acid, ticarcillin, mezlocillin, cefuroxime, cefamandol, cefoxitin, cefotaxime, cefoperazon, ceftazidime, cefsulodin, aztreonam, streptomycin, kanamycin, pipemidic acid, chloramphenicol, erythromicin, pristinamycin, trimethoprim and fosfomycin. O. anthropi is implicated in nosocomial infections. O. anthropi was the species with the greatest resistance to beta-lactamins.

• In-vitro susceptibility of Alcaligenes faecalis compared with those of other Alcaligenes spp. to antimicrobial agents including seven beta-lactams.

  Authors: C Bizet, F Tekaia, A Philippon

  The Journal of antimicrobial chemotherapy. 01/1994; 32(6):907-10.

• Comparative chemotaxonomic studies of mycolic acid-free coryneform bacteria of human origin.

  Authors: C Barreau, F Bimet, M Kiredjian, N Rouillon, C Bizet

  Journal of clinical microbiology. 09/1993; 31(8):2085-90.

  Forty-two clinical isolates were classified as Corynebacterium minutissimum, Corynebacterium striatum, and Corynebacterium CDC group I by the API Coryne system. The chemotaxonomic characteristics ofForty-two clinical isolates were classified as Corynebacterium minutissimum, Corynebacterium striatum, and Corynebacterium CDC group I by the API Coryne system. The chemotaxonomic characteristics of the isolates were determined by thin-layer chromatographic analysis. Twenty-six isolates were found to have a type IV cell wall (meso-di-aminopimelic acid arabinose, galactose) but did not contain mycolic acids. These 26 isolates shared chemotaxonomic characteristics with those of mycolic acid-free reference strains (including the Corynebacterium amycolatum NCFB 2768 type strain, "Corynebacterium asperum," and coryneform CDC groups I2 and F2). The total protein profiles of the isolates determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were similar to each other and to that of the C. amycolatum type strain. The profiles of the reference strains "Corynebacterium asperum" (CIP 100836, CIP 80.54, CIP 79.37, CIP 52.13), coryneform bacteria CDC groups I2 and F2 (CDC F5771, F5890, G723, G1970), and C. amycolatum were closely related. Thus, the mycolic acid-negative strains with a chemotype IV wall may belong to a single taxon. DNA hybridization studies could confirm this hypothesis. The present study shows the importance of chemotaxonomic analysis for verifying strain identifications and completing results from biochemical tests, particularly for coryneform bacteria.

• [Characterization of Alcaligenes species using analysis of esterase electrophoretic polymorphism and analysis of antibiotic resistance profiles]

  Authors: C Bizet, B Picard, A Philippon, P Goullet

  Pathologie-biologie. 06/1992; 40(5):591-8.

  The species of an Alcaligenes bacterial strain may be difficult to determine on the basis of conventional phenotype features. Esterase pattern analysis using acrylamide-agar gel electrophoresis andThe species of an Alcaligenes bacterial strain may be difficult to determine on the basis of conventional phenotype features. Esterase pattern analysis using acrylamide-agar gel electrophoresis and determination of the antimicrobial resistance profile (agar diffusion method) were performed for A. faecalis (34 strains). A. denitrificans subsp xylosoxydans (16 strains) and A. piechaudi (5 strains). The Cistat program (D2 Software) was used for statistical representation of results. The homogeneous, species-specific esterase patterns ensured correct assignment of each strain to one of the three species. Antimicrobial susceptibility was greatest for A. faecalis which was susceptible to both cephalosporins of all generations and aminoglycosides. A. xylosoxydans was the species with the greatest resistance to antimicrobials. A. piechaudii exhibited intermediate susceptibility.

• In vivo selection of a cephamycin-resistant, porin-deficient mutant of Klebsiella pneumoniae producing a TEM-3 beta-lactamase.

  Authors: B Pangon, C Bizet, A Buré, F Pichon, A Philippon, B Regnier, L Gutmann

  The Journal of infectious diseases. 06/1989; 159(5):1005-6.

• [Comparative study of the penetration of penicillin V, amoxicillin, cefaclor and josamycin in the tonsils]

  Authors: C Bizet, B Pangon, N Moisy, J Bizet, A Bure, P Gehanno

  Pathologie-biologie. 05/1989; 37(4):273-5.

  Fourty patients undergoing tonsillectomy for recurrent tonsillitis were administered penicillin V, amoxicillin, cefaclor or josamycin. Antibiotic concentrations in serum and tonsillar tissues wereFourty patients undergoing tonsillectomy for recurrent tonsillitis were administered penicillin V, amoxicillin, cefaclor or josamycin. Antibiotic concentrations in serum and tonsillar tissues were determined by microbiological assay. Cefaclor demonstrated a superior diffusion than penicillin V and amoxicillin, but nevertheless inferior to that of josamycin.

• [Retrospective bacteriological study of mycobacterial infections in patients with acquired immunodeficiency syndrome]

  Authors: B Pangon, C Michon, C Bizet, C Perronne, C Katlama, C Marche, V Lévy-Frébault, A Buré

  Presse médicale (Paris, France : 1983). 06/1988; 17(19):945-8.

  The main species of mycobacteria isolated in 62 of the 316 acquired immunodeficiency syndrome patients admitted to the Claude Bernard Hospital, Paris, between January, 1983 and October, 1986 wereThe main species of mycobacteria isolated in 62 of the 316 acquired immunodeficiency syndrome patients admitted to the Claude Bernard Hospital, Paris, between January, 1983 and October, 1986 were studied retrospectively according to their site of isolation and their pathogenic role. Mycobacterium tuberculosis was isolated in 19 cases (from pulmonary specimens in 17 cases); this species was present in 59 percent of our African patients as against 20 percent of our European patients. M. avium intracellulare was isolated in 33 cases (17 from blood, 12 from the lung and 11 from the gastrointestinal tract) and was found in 55 p. 100 of our European patients. Other species that were isolated less frequently were M. xenopi (5 cases), M. kansasii (3 cases), M. aurum, M. chelonae, M. fortuitum, M. gordonae, M. simiae and M. terrae (1 case each). Post mortem specimens obtained from 110 acquired immunodeficiency syndrome patients were cultivated during the same period. In 20 patients, at least one specimen was positive for a mycobacterium: M. tuberculosis in 2 cases, M. avium intracellulare in 18 cases. Twenty-nine of the 33 patients in whom M. avium intracellulare was isolated were considered a posteriori as being infected by this organism. The therapeutic approach varies according to the species involved. No treatment seems to be truly effective against M. avium intracellulare. Pending the results of cultures, no direct bacteriological examination can provide information on the mycobacterial species concerned; however, a conventional antituberculosis treatment may be instituted, particularly in patients from Africa or Haiti.

• Recovery of Moraxella urethralis from clinical material.

  Authors: C Bizet, A Buré

  European journal of clinical microbiology. 01/1988; 6(6):692-3.

• Distinctive electrophoretic pattern of esterases produced by Alcaligenes species.

  Authors: C Bizet, B Picard, M Kiredjian, P Goullet

  Research in microbiology. 144(3):221-7.

  The esterases produced by 34 strains of Alcaligenes faecalis, 16 strains of A. denitrificans subsp. xylosoxydans, 5 strains of A. piechaudii and 10 strains of A. denitrificans subsp. denitrificansThe esterases produced by 34 strains of Alcaligenes faecalis, 16 strains of A. denitrificans subsp. xylosoxydans, 5 strains of A. piechaudii and 10 strains of A. denitrificans subsp. denitrificans were analysed by horizontal polyacrylamide-agarose gel electrophoresis. These enzymes were distinguished by their spectra of hydrolytic activity towards 5 synthetic substrates (hydrolytic type) and their electrophoretic mobilities (electrophoretic type). Four hydrolytic types of esterases were produced by A. faecalis, three hydrolytic types by A. denitrificans subsp. xylosoxydans, three hydrolytic types by A. piechaudii and 14 hydrolytic types by A. denitrificans subsp. denitrificans. Both (hydrolytic and electrophoretic) properties and the pattern of esterases produced by each strain were used to define 8 zymotypes in A. faecalis, 6 zymotypes in A. denitrificans subsp. xylosoxydans, 3 zymotypes in Alcaligenes piechaudii and 10 zymotypes in Alcaligenes denitrificans subsp. denitrificans. These results permit precise identification of strains within the four species of Alcaligenes and provide useful epidemiological markers.