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ABSTRACT: Coupling hyperspectral image data having different spatial resolutions using Multiple Co-inertia Analysis, Chemometrics and Intelligent Laboratory Systems (2012), doi: 10.1016/j.chemolab.2012.04.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Chemometrics and Intelligent Laboratory Systems 04/2012; · 1.92 Impact Factor
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ABSTRACT: In addition to the starchy endosperm, a specialized tissue accumulating storage material, the endosperm of wheat grain, comprises the aleurone layer and the transfer cells next to the crease. The transfer cells, located at the ventral region of the grain, are involved in nutrient transfer from the maternal tissues to the developing endosperm. Immunolabeling techniques, Raman spectroscopy, and synchrotron infrared micro-spectroscopy were used to study the chemistry of the transfer cell walls during wheat grain development. The kinetic depositions of the main cell wall polysaccharides of wheat grain endosperm, arabinoxylan, and (1-3)(1-4)-β-glucan in transfer cell walls were different from kinetics previously observed in the aleurone cell walls. While (1-3)(1-4)-β-glucan appeared first in the aleurone cell walls at 90°D, arabinoxylan predominated in the transfer cell walls from 90 to 445°D. Both aleurone and transfer cell walls were enriched in (1-3)(1-4)-β-glucan at the mature stage of wheat grain development. Arabinoxylan was more substituted in the transfer cell walls than in the aleurone walls. However, arabinoxylan was more feruloylated in the aleurone than in the transfer cell walls, whatever the stage of grain development. In the transfer cells, the ferulic acid was less abundant in the outer periclinal walls while para-coumarate was absent. Possible implications of such differences are discussed.
Planta 11/2010; · 3.00 Impact Factor
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J. Cereal Sci,. 01/2009; 49:354-362.
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ABSTRACT: Endosperm cell walls of cultivars of wheat (Triticum aestivum L.) selected for their endosperm texture (two soft and two hard) were analysed in situ by Fourier transform infrared (FTIR) microspectroscopy. FTIR imaging coupled with statistical analysis was used to map the compositional and structural heterogeneity within transverse sections from which cell contents had been removed by sonication. In the majority of grains analysed, two distinct populations of endosperm cells could be identified by spectral features that were related to cell morphology and age, regardless of cultivar. The main cell-wall component responsible for these differences was the polysaccharide arabinoxylan. In a few samples, this heterogeneity was absent, for reasons that are not understood, but this was not correlated to endosperm texture or growth conditions. Within the same population of endosperm cells, cell walls of hard endosperm could be distinguished from those of soft endosperm by their spectral features. Compared to hard cultivars, the peripheral endosperm of soft cultivars was characterised by a higher amount of polymer, whose spectral feature was similar to water-extractable arabinoxylan. In contrast, no specific compound has been identified in the central endosperm: structural differences within the polysaccharides probably contribute to the distinction between hard and soft cultivars. In developing grain, a clear difference in the composition of the endosperm cell walls of hard and soft wheat cultivars was observed as early as 15 days after anthesis.
Planta 04/2005; 220(5):667-77. · 3.00 Impact Factor
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http://dx.doi.org/10.1051/IUFoST:20061361.
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ABSTRACT: A new methodology based on pulsed lasers has been developed in order to estimate wheat outer layer mechanical properties without sample preparation. Laser experiments were carried out with an Argon Fluoride (λ = 193 nm) excimer laser source delivering pulses of 15 ns duration. Wheat grains from two cultivars were irradiated by single laser pulses with a quasi-uniform irradiation and two fluences (2.5 and 5 J cm−2). The ablation flux was characterized by environmental scanning electron microscopy before measuring the removed material on cross-sections observed by confocal scanning laser microscopy. Specific image treatment was carried out to obtain the ablation flux (amount of removed matter per pulse). Pericarp, seed coat and aleurone layer were gradually ablated under the laser conditions used in this work. Their ablation thresholds were different and could be related to tissue cohesion. Specific behaviour of seed coat layer (8 μm) could be emphasised with this technique. Pulsed laser ablation could be a potential methodology to reveal indirectly wheat grain layer cohesion.
Journal of Cereal Science.
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ABSTRACT: Measurement of biochemical markers allows the quantification of wheat (Triticum spp.) grain tissue proportions in milling fractions. In order to evaluate the ability of extending this methodology to an unknown wheat grain batch, the variability of the markers in the different tissues was assessed on various wheat cultivars. Ferulic acid trimer amounts in the outer pericarp ranged from 0.97 to 1.67 μg mg−1 (dm) with an average value equal to 1.31 μg mg−1 (dm). Alkylresorcinols amounts in a composite layer, including the testa, the inner pericarp and the nucellar epidermis, ranged from 10.5 to 16.7 mg g−1 (dm), with an average value equal to 14.0 mg g−1 (dm). In the aleurone layer, phytic acid amounts ranged from 94.9 to 187.2 mg g−1 (dm) with an average value equal to 152 mg g−1 (dm) whereas, para-coumaric acid ranged from 0.08 to 0.29 μg mg−1 with an average level of 0.18 μg mg−1. In the embryonic axis, wheat germ agglutinin ranged from 879 μg g−1 to 2086 μg g−1 with an average value of 1487 μg g−1. The impact of this variability on tissue proportion determination was evaluated and a strategy to decrease the prediction error was suggested. Percentages of the outer pericarp, intermediate layer (including the testa), aleurone layer and embryonic axis within grains were calculated and their variability discussed.
Journal of Cereal Science 53(3):306-311. · 2.07 Impact Factor
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ABSTRACT: NIR spectroscopy presents a huge interest in exploring chemical changes during dough mixing. The aim of the present study is to investigate the potential of 2D correlation spectroscopy (2D COS) and moving-window 2D (MW2D) correlation spectroscopy to explore the time dependence of NIR spectral responses during wheat flour dough mixing. NIR spectra were continuously recorded (between 1400 and 2325 nm) during mixing of bread type-dough (based on flour, water and yeast), using an FT-NIR spectrometer with a deported probe. The probe was positioned inside the mixer in contact with the dough. The 2D spectra calculated using raw and second derivative NIR spectra were interpreted in terms of physico-chemical events. Nine different industrial flours were used as raw material to validate the analysis. The results obtained using the 2D COS and the MW2D methods give the possibility to ascribe chemical vibrations (starch, water and gluten) to NIR absorbance changes occurring during dough mixing. The analysis of the NIR spectra identified wavelength shift associated to both dough “free water” and protein secondary structure modifications. During this study, only the MW2D method allowed to identify clearly the time dependence of physico-chemical mechanisms from NIR variation bands.
Journal of Cereal Science.
