Baoru Yin

Publications (2)3.07 Total impact

• Article: Stable nano-sized emulsions produced from soy protein and soy polysaccharide complexes.

  Baoru Yin, Wei Deng, Keke Xu, Luwen Huang, Ping Yao
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  ABSTRACT: In this research, a simple, green and effective strategy was developed to produce long-term stable oil in water emulsion from soy protein and soy polysaccharide. Soy protein and soy polysaccharide formed dispersible complexes at pH around 3.25 aqueous solution through electrostatic and hydrophobic interactions. A high pressure homogenization produced the protein/polysaccharide complex emulsion having a droplet size about 250 nm. A heat treatment of the emulsion resulted in the protein denaturation, forming irreversible oil-water interfacial films composed of soy protein/soy polysaccharide complexes. The droplets of the emulsion were characterized by dynamic light scattering, ζ-potential, transmission electron microscopy, polysaccharide digestion via pectinase, and confocal laser scanning microscopy observation via dual fluorescence probes. As a result of the polysaccharide being fixed on the droplet surface, the emulsions exhibited long-term stability in the media containing pH values of 2-8 and 0.2 mol/L NaCl. The stable soy protein/soy polysaccharide complex emulsion is a suitable food-grade delivery system in which lipophilic bioactive compounds can be encapsulated.
  Journal of Colloid and Interface Science 05/2012; 380(1):51-9. · 3.07 Impact Factor
• Article: [Preparation and high performance liquid chromatographic separation of casein peptides].

  Huanxin Cai, Baoru Yin, Ping Yao
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  ABSTRACT: A preparative reversed-phase high performance liquid chromatography-electrospray ionization mass spectrometry (RP-HPLC/ESI-MS) method to obtain low cost casein peptides was developed. Casein was hydrolyzed by trypsin and the hydrolysate was analyzed by RP-HPLC/ESI-MS firstly. The gradient elution program in an analytical column was optimized to achieve a better separation. The effect of pH of the mobile phase on the separation was also investigated. Then, the optimal analytical conditions were applied in a preparative column directly. In each loading of the hydrolysate, several pure peptide fractions were collected automatically by the inductions of ultraviolet absorbance and mass spectrometry signals together. The process is simple and effective. The influence of the loading amount in a preparative column was investigated intensively. For the collection consisting of hydrophilic peptides that were poorly separated in the primary process, secondary separation was performed. By changing the gradient elution, several different pure peptides were obtained.
  Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 07/2010; 28(7):637-43.