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ABSTRACT: The effect of glycerol, glucose, and starch as carbon sources on the antioxidant defense system such as superoxide dismutase (SOD) and catalase (CAT) activities, pyruvate levels, and membrane lipid peroxidation (LPO) levels of Streptomyces sp. M4018, after isolation from the rhizosphere samples of Colutea arborescens and identification as a strain of S. hiroshimensis based on phenotypic and genotypic characteristics, were investigated. As an antioxidant defense enzyme, SOD activities increased up to 20 g/L of glycerol and 15 g/L of starch, while they showed negative correlation with glucose concentration. CAT activity variations of glycerol- and glucose-supplemented mediums showed significant positive correlations with the trend of SOD activities. However, CAT activity, in contrast to SOD, in Streptomyces sp. M4018 tended to decrease as the starch concentration increased. The production of pyruvate increased with respect to glycerol and starch up to 15 g/L, while it was positively correlated with glucose concentration. The highest pyruvate production was seen at 20 g/L glucose. Membrane LPO levels were negatively correlated with the activities of SOD and CAT enzymes, and the minimum LPO level was determined at 5 g/L of glucose, where SOD and CAT activities reached their maximum levels. Nevertheless, the higher SOD and CAT activities in a wider range of incubation period compared to the beginning by resulting in insignificant increases in membrane LPO levels showed the unusual antioxidant response capacities of the in Streptomyces sp. M4018 against the potentially deleterious effects of reactive oxygen species (ROS) for glycerol, glucose, and starch as carbon sources.
Preparative Biochemistry & Biotechnology 07/2012; 42(4):322-34. · 0.47 Impact Factor
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ABSTRACT: The alterations of organic acids citrate, α-ketoglutarate, succinate, fumarate, malate production together with isocitrate
lyase activity as a glyoxalate shunt enzyme, and antibiotic production of Streptomyces sp M4018 were investigated in relation to changes in the glucose, glycerol and starch concentrations (5–20g/L) after identification
as a strain of Streptomyces hiroshimensis based on phenotypic and genotypic characteristics. The highest intracellular citrate and α-ketoglutarate levels in 20g/l
of glucose, glycerol, and starch mediums were 399.47 ± 4.78, 426.93 ± 6.40, 355.84 ± 5.38ppm and 444.81 ± 5.12, 192.96 ± 2.26,
115.20 ± 2.87ppm, respectively. The highest succinate, malate, and fumarate levels were also determined in 20g/l of glucose
medium as 548.9 ± 11.21, 596.15 ± 8.26, and 406.42 ± 6.59ppm and the levels were significantly higher than the levels in
glycerol and starch. Extracellular organic acid levels measured also showed significant correlation with carbon source concentrations
by showing negative correlation with pH levels of the growth medium. The antibiotic production of Streptomyces sp. M4018 was also higher in glucose medium as was the case also for organic acids when compared with glycerol. On the other
hand, there is no production in starch.
Keywords
Streptomyces sp.–TCA cycle–Glyoxalate cycle–Antibiotic production
Applied Biochemistry and Biotechnology 04/2012; 164(3):318-337. · 1.94 Impact Factor
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ABSTRACT: To determine the variations of growth, some key enzyme activities such as glucose kinase (GK), glucose-6-phosphate dehydrogenase
(G6PDH), α-ketoglutarate dehydrogenase (KGDH), and isocitrate lyase (ICL) besides metabolite levels of pyruvate and antibiotic
production of newly isolated Streptomyces sp. M3004 were grown in culture media which contain 10–20g/l concentration with either glucose or glycerol as carbon source.
Biomass and intracellular glucose and glycerol levels of Streptomyces sp. M3004 showed positive correlation with the concentration of these carbon sources, and these levels were higher in glucose
compared with the glycerol-supplemented mediums. GK, G6PDH, and KGDH activities showed marked correlation with the concentration
of both glucose and glycerol, and the activity levels were 4.14-, 1.47-, and 1.27-fold higher in glucose than glycerol. A
key enzyme of the glyoxalate cycle, ICL activities decreased with increasing glucose concentrations from 10 to 20g/l, but
increased up to 15g/l of glycerol. The positive correlations were also determined between intracellular glucose and glycerol
levels besides pyruvate and protein variations with respect to concentrations of the carbon sources. Antibacterial activities
of Streptomyces sp. M3004 reached maximum on the stationary phase, while it did not change significantly with respect to glucose and glycerol.
Keywords
Streptomyces sp.–Carbon sources–Carbohydrate metabolism–Protein–Antibiotic
Applied Biochemistry and Biotechnology 04/2012; 165(1):369-381. · 1.94 Impact Factor
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ABSTRACT: To determine the variations of growth, some key enzyme activities such as glucose kinase (GK), glucose-6-phosphate dehydrogenase (G6PDH), α-ketoglutarate dehydrogenase (KGDH), and isocitrate lyase (ICL) besides metabolite levels of pyruvate and antibiotic production of newly isolated Streptomyces sp. M3004 were grown in culture media which contain 10-20 g/l concentration with either glucose or glycerol as carbon source. Biomass and intracellular glucose and glycerol levels of Streptomyces sp. M3004 showed positive correlation with the concentration of these carbon sources, and these levels were higher in glucose compared with the glycerol-supplemented mediums. GK, G6PDH, and KGDH activities showed marked correlation with the concentration of both glucose and glycerol, and the activity levels were 4.14-, 1.47-, and 1.27-fold higher in glucose than glycerol. A key enzyme of the glyoxalate cycle, ICL activities decreased with increasing glucose concentrations from 10 to 20 g/l, but increased up to 15 g/l of glycerol. The positive correlations were also determined between intracellular glucose and glycerol levels besides pyruvate and protein variations with respect to concentrations of the carbon sources. Antibacterial activities of Streptomyces sp. M3004 reached maximum on the stationary phase, while it did not change significantly with respect to glucose and glycerol.
Applied biochemistry and biotechnology 04/2011; 165(1):369-81. · 1.94 Impact Factor
