Anna Bajek

Publications of Anna Bajek

• Does the Mesenchymal Stem Cells aging limit their potential application in clinical practice?

  Authors: Anna Bajek, Mateusz Czerwinski, Joanna Olkowska, Natalia Gurtowska, Tomasz Kloskowski, Tomasz Drewa

  Aging clinical and experimental research. 05/2012;

  Mesenchymal stem cells (MSCs) are in the center of attention of many investigators due to easy isolation from many tissues. MSCs capability to differentiate into many cell types makes them a startingMesenchymal stem cells (MSCs) are in the center of attention of many investigators due to easy isolation from many tissues. MSCs capability to differentiate into many cell types makes them a starting point of many new therapies, especially in tissue engineering. However, understanding the process of MSCs aging is crucial for selecting donors for cellular therapies, which is necessary for successful treatment. Cell changes can be divided into three major groups. Changes which affect their proliferate rate, differentiation capability and genome stability are leading decrease their usefulness in new therapies. There are many tools that can be used to describe and measure some feature of aging in MSCs but essence of this process still eludes. The aim of this review is to take a deep look into influence of donor age and in vitro aging on MSCs properties.

• [Mesenchymal stem cells as a therapeutic tool in tissue and organ regeneration].

  Authors: Anna Bajek, Joanna Olkowska, Tomasz Drewa

  Postȩpy higieny i medycyny doświadczalnej (Online). 01/2011; 65:124-32.

  Tissue engineering is an interdisciplinary field that offers new opportunities for regeneration of diseased and damaged tissue with the use of many different cell types,including adult stem cells. InTissue engineering is an interdisciplinary field that offers new opportunities for regeneration of diseased and damaged tissue with the use of many different cell types,including adult stem cells. In tissue engineering and regenerative medicine the most popular are mesenchymal stem cells (MSCs) isolated from bone marrow. Bone marrow mesenchymal stem cells are a potential source of progenitor cells for osteoblasts, chondroblasts, adipocytes, skeletal muscles and cardiomyocytes. It has also been shown that these cells can differentiate into ecto- and endodermal cells, e.g. neuronal cells, glial cells, keratinocytes and hepatocytes. The availability of autologous MSCs, their proliferative potential and multilineage differentiation capacity make them an excellent tool for tissue engineering and regenerative medicine. The aim of this publication is to present characteristic and biological properties of mesenchymal stem cells isolated from bone marrow.

• The influence of ciprofloxacin on viability of A549, HepG2, A375.S2, B16 and C6 cell lines in vitro.

  Authors: Tomasz Kloskowski, Natalia Gurtowska, Monika Nowak, Romana Joachimiak, Anna Bajek, Joanna Olkowska, Tomasz Drewa

  Acta poloniae pharmaceutica. 68(6):859-65.

  Ciprofloxacin is a chemotherapeutic agent mainly used in the treatment of the pulmonary and urinary tract infections but is also known for its anticancer properties. The aim of these study was toCiprofloxacin is a chemotherapeutic agent mainly used in the treatment of the pulmonary and urinary tract infections but is also known for its anticancer properties. The aim of these study was to check the anticancer effect of ciprofloxacin on selected five cell lines. Human non-small cell lung cancer line A549, human hepatocellular carcinoma line HepG2, human and mouse melanoma lines (A375.S2 and B16) and rat glioblastoma line C6 were used for evaluation of cytotoxic properties of ciprofloxacin (in concentration range: 10-1000 microg/mL). Viability was established using trypan blue assay and MTT. Ciprofloxacin induced morphological changes and decreased viability of A549 cells in a concentration and time dependent manner. In case of A375.S2 and B16 cell lines, cytotoxicyty of ciprofloxacin was observed but we were not able to eradicate all cells from A375.S2 and B16 cultures. HepG2 line was sensitive to ciprofloxacin, but this effect was independent from concentration and incubation time. The C6 cells were insensitive to ciprofloxacin. Our results showed that ciprofloxacin can be potentially used for the experimental adjunctive therapy of lung cancer.