An-An Liu

Publications (2)13.85 Total impact

• Article: Surface labeling of enveloped viruses assisted by host cells.

  Bi-Hai Huang, Yi Lin, Zhi-Ling Zhang, Fangfang Zhuan, An-An Liu, Min Xie, Zhi-Quan Tian, Zhenfeng Zhang, Hanzhong Wang, Dai-Wen Pang
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  ABSTRACT: Labeling of virus opens new pathways for the understanding of viruses themselves and facilitates the utilization of viruses in modern biology, medicine, and materials. Based on the characteristic that viruses hijack their host cellular machineries to survive and reproduce themselves, a host-cell-assisted strategy is proposed to label enveloped viruses. By simply feeding Vero cells with commercial 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt) (Biotin-Cap-PE), we obtained biotinylated Vero cells whose membrane systems were modified with biotin. Subsequently, pseudorabies viruses (PrV) were cultivated in the biotinylated Vero cells, and the PrV progenies were spontaneously labeled with Biotin-Cap-PE during viral natural assembly process. Since the viral natural assembly process was employed for the labeling, potential threats of genetic engineering and difficulties in keeping viral natural bioactivity were avoided. Importantly, this labeling strategy for enveloped virus greatly reduces the technical complexity and allows researchers from different backgrounds to apply it for their specified demands.
  ACS Chemical Biology 02/2012; 7(4):683-8. · 6.45 Impact Factor
• Article: A multicomponent recognition and separation system established via fluorescent, magnetic, dualencoded multifunctional bioprobes.

  Jun Hu, Min Xie, Cong-Ying Wen, Zhi-Ling Zhang, Hai-Yan Xie, An-An Liu, Yong-Yong Chen, Shi-Ming Zhou, Dai-Wen Pang
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  ABSTRACT: Accurate and rapid recognition and separation of multiple types of biological targets such as molecules, cells, bacteria or viruses from complex sample mixtures is of great importance for a wide range of diagnostic and therapeutic strategies. To achieve this goal, a set of fluorescent, magnetic, dual-encoded multifunctional bioprobes has been constructed by co-embedding different-sized quantum dots and varying amounts of γ-Fe(2)O(3) magnetic nanoparticles into swollen poly(styrene/acrylamide) copolymer nanospheres. The dual-encoded bioprobes, which possessed different photoluminescent property and magnetic susceptibility, were proven to be capable of simultaneously recognizing and separating multiple components from a complex sample when three kinds of lectins were used as the targets. The lectins were separated with high efficiency and kept their bioactivity during the process. Compared to the conventional batchwise separation, this method does not require a large number of sequential reaction steps, which is economical of time and can be very reagent-saving. By combining the multiplexing capability of quantum dots with the superparamagnetic properties of iron oxide nanoparticles, this dual-encoded technique is expected to open new opportunities in high-throughput and multiplex bioassays, such as cell sorting, proteomical and genomical applications, drug screening etc.
  Biomaterials 11/2010; 32(4):1177-84. · 7.40 Impact Factor